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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
Autores:  Lopes,Denilce Meneses
Campos,Lúcio Antônio de Oliveira
Salomão,Tânia Maria Fernandes
Tavares,Mara Garcia
Data:  2010-01-01
Ano:  2010
Palavras-chave:  Melipona
Stingless bees
Resumo:  Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572010005000017
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.33 n.2 2010
Direitos:  info:eu-repo/semantics/openAccess

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