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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database
Autores:  Freitas,Patrícia Domingues de
Pinheiro,Allysson Pontes
Silva,Thiago Buosi
Galetti Junior,Pedro Manoel
Data:  2007-01-01
Ano:  2007
Palavras-chave:  Crustacea
Microsatellite structure
Penaeidae
SSR
Resumo:  We performed an in silico analysis of all microsatellites so far described for penaeid shrimp and for which the polymorphic behavior has previously been analyzed. The objective of the study was to evaluate the structural characteristics of these microsatellites and identifying patterns which allow the characterization of the nature of these sequences in the penaeid genome. All data were compiled in a free-access database specially constructed for this study. Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed and simple and compound microsatellites with di-, tri-, tetra-, penta- and hexanucleotide motifs were found. Dinucleotides and trinucleotides were the most frequent motifs among both the simple and the compound microsatellites. Although a certain bias related to different microsatellite isolation methodology could not be discarded, it is possible that part of this microsatellite abundance reflects some degree of conservation of microsatellite motifs among the different species. There was a pronounced motif variability within and between species, indicating high differentiation dynamism of these repetitions in this animal group. This study not only sheds light on the structure of the microsatellites present in the penaeid shrimp genome but also resulted in the free-access Penaeid Shrimp Microsatellite Database (available at http://www.shrimp.ufscar.br) which may be very useful for optimizing the use of these microsatellites.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600027
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572007000600027
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.30 n.4 2007
Direitos:  info:eu-repo/semantics/openAccess
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