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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Xylella fastidiosa gene expression analysis by DNA microarrays
Autores:  Travensolo,Regiane F.
Carareto-Alves,Lucia M.
Costa,Maria V.C.G.
Lopes,Tiago J.S.
Carrilho,Emanuel
Lemos,Eliana G.M.
Data:  2009-01-01
Ano:  2009
Palavras-chave:  Xylella fastidiosa
DNA microarray
Gene expression
Resumo:  Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM2 and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa genome-wide transcriptome analysis, the present work observed the differential expression of several classes of genes (energy, protein, amino acid and nucleotide metabolism, transport, degradation of substances, toxins and hypothetical proteins, among others). The understanding of expressed genes in these two different media will be useful in comprehending the metabolic characteristics of X. fastidiosa, and in evaluating how important certain genes are for the functioning and survival of these bacteria in plants.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200022
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572009005000038
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.32 n.2 2009
Direitos:  info:eu-repo/semantics/openAccess
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