| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
SSD - a free software for designing multimeric mono-, bi- and trivalent shRNAs
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| Autores: |
Carli,Gabriel José de
Rotela,Abdon Troche
Lubini,Greice
Contiliani,Danyel Fernandes
Candia,Nidia Benítez
Depintor,Thiago S.
Abreu,Fabiano Carlos Pinto de
Simões,Zilá Luz Paulino
Ríos,Danilo Fernández
Pereira,Tiago Campos
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| Data: |
2020-01-01
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| Ano: |
2020
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| Palavras-chave: |
Gene silencing
SiRNA
Multimeric
ShRNA
Free software
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| Resumo: |
Abstract RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development of therapeutic strategies and generation of biotechnological products. Here we present a free software tool for the rational design of RNAi effectors, named siRNA and shRNA designer (SSD). SSD incorporates our previously developed software Strand Analysis to construct template DNAs amenable for the large scale production of mono-, bi- and trivalent multimeric shRNAs, via in vitro rolling circle transcription. We tested SSD by creating a trivalent multimeric shRNA against the vitellogenin gene of Apis mellifera. RT-qPCR analysis revealed that our molecule promoted a decrease in more than 50% of the target mRNA, in a dose-dependent manner, when compared to the control group. Thus, SSD software allows the easy design of multimeric shRNAs, for single or multiple simultaneous knockdowns, which is especially interesting for studies involving large amounts of double-stranded molecules.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100802
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/1678-4685-gmb-2019-0300
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.43 n.1 2020
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| Direitos: |
info:eu-repo/semantics/openAccess
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