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Provedor de dados:  PAB
País:  Brazil
Título:  Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans
Autores:  Pinheiro,Patrícia Valle
Faria,Josias Corrêa de
Nogueira,Elsa Oliveira Paranaguá e Lago
Aragão,Francisco José Lima
Data:  2009-09-01
Ano:  2009
Palavras-chave:  Phaseolus
BCMNV
Genetic engineering
Genetic variability
Transgene genotypic stability
Resumo:  The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specific oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close to 80% of the characteristics of the recurrent parent, as determined by the random amplified DNA markers used, besides maintaining important traits such as resistance to BCMNV. The presence of the transgene did not cause any detectable undesirable effect in the evaluated progenies.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000900015
Editor:  Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira
Relação:  10.1590/S0100-204X2009000900015
Formato:  text/html
Fonte:  Pesquisa Agropecuária Brasileira v.44 n.9 2009
Direitos:  info:eu-repo/semantics/openAccess
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