| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines
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| Autores: |
Petrillo,Caroline Pereira
Carneiro,Newton Portilho
Purcino,Antônio Álvaro Corsetti
Carvalho,Carlos Henrique Siqueira
Alves,José Donizeti
Carneiro,Andréa Almeida
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| Data: |
2008-03-01
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| Ano: |
2008
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| Palavras-chave: |
Zea mays
Ammonium glufosinate
Beta-glucuronidase
Immature embryos
Phosphinotricin acetyl transferase
Plant transformation
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| Resumo: |
The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000300012
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/S0100-204X2008000300012
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.43 n.3 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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