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Provedor de dados:  Rev. Bras. Bot.
País:  Brazil
Título:  Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis
Autores:  FILIPPI,SILVIA BALBÃO
APPEZZATO-DA-GLÓRIA,BEATRIZ
RODRIGUEZ,ADRIANA PINHEIRO MARTINELLI
Data:  2001-12-01
Ano:  2001
Palavras-chave:  Histology
Somatic embryos
Dicamba
Picloram
Banana
Resumo:  The present work analyzes the behavior of banana explants, cv. Nanicão (Musa spp. Group AAA) regarding somatic embryogenesis induction treatments with several auxins. Longitudinal segments of shoot meristematic apices of micropropagated banana plantlets cultivated and rooted in vitro were introduced in culture medium containing dicamba, picloram, 2,4-D or NAA in different concentrations. Explant samples were collected at 0, 7 and 10 days and prepared for light microscopy. Histological sections were used for comparison of the histological changes occurring after induction treatment with different auxins. Embryogenic response was observed only in treatments with picloram or dicamba, with distinct embryogenic regions observed at 14 and 21 days in culture, respectively. Histological sections of embryogenic regions of the explant at 26 days in culture revealed the formation of meristematic regions, structures with multiple root meristems, and somatic embryos at early globular stages. Embryo-like structures morphologically similar to Musa balbisiana zygotic embryos were sectioned and showed a lack of apical meristems and absence of procambial differentiation. These results indicate the induction of non-functional somatic embryos and the need for more studies on developmental aspects and maturation treatments for optimization of the process.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500015
Editor:  Sociedade Botânica de São Paulo
Relação:  10.1590/S0100-84042001000500015
Formato:  text/html
Fonte:  Brazilian Journal of Botany v.24 n.4 suppl.0 2001
Direitos:  info:eu-repo/semantics/openAccess
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