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Provedor de dados:  Rev. Bras. Ciênc. Avic.
País:  Brazil
Título:  Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil
Autores:  Guerreiro,AN
Moraes,CCG
Marinho,ANR
Barros,BCV
Bezerra,DAM
Bandeira,RS
Silva,RR
Rocha,DCC
Meneses,AMC
Luz,MA
Paz,GS
Mascarenhas,JDP
Data:  2018-03-01
Ano:  2018
Palavras-chave:  Animals
Amazônia
Picobirnavirus
Reovirus
Rotavirus
RT-PCR.
Resumo:  ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100161
Editor:  Fundação APINCO de Ciência e Tecnologia Avícolas
Relação:  10.1590/1806-9061-2017-0589
Formato:  text/html
Fonte:  Brazilian Journal of Poultry Science v.20 n.1 2018
Direitos:  info:eu-repo/semantics/openAccess
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