| Registro completo |
| Provedor de dados: |
Rev. Bras. Ciênc. Avic.
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| País: |
Brazil
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| Título: |
Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
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| Autores: |
Oliveira,GH
Berchieri Jr,A
Montassier,HJ
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| Data: |
2006-03-01
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| Ano: |
2006
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| Palavras-chave: |
Chicken
ELISA
Enzyme
Salmonella sp
Serology
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| Resumo: |
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008
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| Editor: |
Fundação APINCO de Ciência e Tecnologia Avícolas
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| Relação: |
10.1590/S1516-635X2006000100008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Poultry Science v.8 n.1 2006
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| Direitos: |
info:eu-repo/semantics/openAccess
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