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Provedor de dados:  Rev. Bras. Ciênc. Avic.
País:  Brazil
Título:  Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil
Autores:  Pinto,LB
Ometto,T
Araújo,J
Thomazelli,LM
Seixas,MM
Barbosa,CM
Ramos,DGS
Melo,ALT
Pinho,JB
Durigon,EL
Aguiar,DM
Data:  2016-06-01
Ano:  2016
Palavras-chave:  Pantanal birds
Infectious Diseases
RT-PCR
QRT-PCR
Virus
Resumo:  ABSTRACT The Pantanal is the world's largest wetland biome with a seasonal flood pulse that attracts a great diversity of birds, many of which are migratory. Birds can be natural reservoirs Influenza A, West Nile and Newcastle Disease viruses. However, the occurrence of carriers for these viruses in the Pantanal was not verified yet. The present study evaluated the occurrence of natural infection by Influenza A, WN and ND virus of birds in the municipality of Poconé, a subregion of the Pantanal in the state of Mato Grosso, Brazil. A total of 76 birds belonging to 11 orders and 20 families were captured using mist nets. The most representative order was Passeriformes, followed by the other nine orders, which included Columbiformes, Psittaciformes, Charadriiformes and Anseriformes. The most representative family was Thamnophilidae, with 16 individuals (21.0%), followed by the family Tyrannidae with 10 individuals (7.6%) and the family Furnariidae, with eight individuals (10.5%). The bird species were identified, and cloacal and tracheal swab samples were collected. The samples were subjected to RNA extraction and tested for the presence of the three agents by real-time polymerase chain reaction (qRT-PCR). All the sampled birds were considered healthy, had no clinical sign of infection, and were tested negative for the three viruses. Based on our findings, we can conclude that Influenza, West Nile and Newcastle Disease viruses were absent from the samples in this region of the Pantanal wetlands during the period of this study.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200291
Editor:  Fundação APINCO de Ciência e Tecnologia Avícolas
Relação:  10.1590/1806-9061-2015-0111
Formato:  text/html
Fonte:  Brazilian Journal of Poultry Science v.18 n.2 2016
Direitos:  info:eu-repo/semantics/openAccess
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