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Provedor de dados:  95
País:  Brazil
Título:  Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds
Autores:  Barrocas,Ellen Noly
Machado,José da Cruz
Almeida,Mirella Figueiró de
Botelho,Luana Silva
Pinho,Édila Vilela de Resende Von
Data:  2012-06-01
Ano:  2012
Palavras-chave:  Fungus
Molecular detection
Stalk and ear rot
Macrospore leaf spot
Resumo:  Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. The use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. The seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.The primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000200005
Editor:  Associação Brasileira de Tecnologia de Sementes
Relação:  10.1590/S0101-31222012000200005
Formato:  text/html
Fonte:  Revista Brasileira de Sementes v.34 n.2 2012
Direitos:  info:eu-repo/semantics/openAccess
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