Consulta: creatorFacets:"宮原, 和郎"
Registros recuperados: 26
Data/hora: 06/06/2024 02:26:07

Investigation of the Short-Term Effect of Chemonucleolysis with Chondroitinase ABC
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: YAMADA, Kazutaka; TANABE, Shinobu; UENO, Hiroshi; OINUMA, Akemi; TAKAHASHI, Toyomi; MIYAUCHI, Satoshi; SHIGENO, Satoshi; HIROSE, Tsuneo; MIYAHARA, Kazuro; SATO, Motoyoshi; 山田, 一孝; 宮原, 和郎.
Chondroitinase ABC (C-ABC) is expected to be a novel agent for chemonucleolysis. The effect of C-ABC was investigated by magnetic resonance (MR) and radiograph. C-ABC was administered into the lumbar intervertebral disks on the clinically normal beagles (n=5), in a dose of 50 μl (12.5 units as C-ABC). MR scans were performed pre-dose, and 1, 3, 7, 14 and 28 days after administration of C-ABC, and the signal intensity (SI) of the nucleus pulposus was measured. Radiographs were taken pre-dose, and 1, 2, 3, 4, 5, 6, 7, 14 and 28 days post-dose, to evaluate narrowing of the disk space in terms of height index (HI). In addition, the quantity of the chondroitin sulfate (CS) and the hyaluronic acid (HA) in the nucleus pulposus were measured by high performance...
Palavras-chave: Canine; Chemonucleolysis; Chondroitinase ABC (C-ABC); Height index (HI); Magnetic resonance (MR).
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4181

工業用超音波探傷装置の獣医臨床への応用-牛及び馬の蹄測定値について-
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎; 吉田, 千央; 佐藤, 基佳; 広瀬, 恒夫; MIYAHARA, Kazuro.
Ano: 1990
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4078

帯広畜産大学動物医療センター
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎.
獣医さんのアルバムから(94)
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2933

馬の頚椎狭窄性脊髄症の病態生理と診断に関する文献展望
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎; 宮林, 孝仁; 佐藤, 基佳; 中野, 達雄; 広瀬, 恒夫.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4079

第6回日独原虫病シンポジウム参加とミュンヘン大学における大動物診療
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎.
Ano: 1988
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/466

ホルスタイン種牛における肝臓ならびに後大静脈の画像診断所見と静脈圧との関連について
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎; 吉林台.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2211

Lineage Classification of Canine Inheritable Disorders Using Mitochondrial DNA Haplotypes
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: TAKAHASI, Shoko; MIYAHARA, Kazuro; ISHIKAWA, Hirosi; ISHIGURO, Naotaka; SUZUKI, Mitsuyoshi; 宮原, 和郎; 鈴木, 三義.
To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of...
Palavras-chave: D-loop; Haplotype lineage analysis; Mitochondrial DNA.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4066

20-Methylcholanthrene誘発腫瘍担癌マウスに対するトキソプラズマ溶解抗原の治療的投与効果
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎; 本多, 字里子; 戸瀬, 信一; 櫻井, 治久; 五十嵐, 郁男; 齋藤, 篤志; 広瀬, 恒夫; 鈴木, 直義; MIYAHARA, Kazuro.
Therapeutic effects of Toxoplasma lysate antigen (TLA) were studied in mice bearing the tumor in the second passage of 20-methylcholanthrene (MC)-induced tumor cells. Intramuscular administration of TLA 7 days after the tumor-cell inoculation caused apparent inhibition of the tumor growth on day 14. The second treatment facilitated the therapeutic effects. Intravenous transfer of spleen cells prepared from TLA-sensitized mice into tumor-bearing mice also represented the growth inhibitory effects. Prominent effects were seen when the transferred cells were prepared 5 days after sensitization of donor animals. The inhibitory effects were absent in the groups transferred only the adherent cells or the non-adherent cells prepared from sensitized mice. The...
Palavras-chave: Methylcholanthrene-induced tumor; Mouse spleen cell therapeutic effect Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4062

慢性化膿性気管支肺炎に継発した心膜炎のホルスタイン乳牛の1症例
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 松山, 雄喜; 小山, 憲司; 坂田, 貴洋; 古林, 与志安; 松本, 高太郎; 宮原, 和郎; 猪熊, 壽.
3歳7カ月齢のホルスタイン種乳牛が分娩後３日目に元気食欲不振を呈した。頻脈、削痩、脱水および眼球陥凹が著しく、頚静脈怒張、発咳、肺音粗励および膿性鼻汁排出の症状も認められた。臨床症状、白血球増多症、慢性炎症所見および心エコー検査により心膜炎を疑ったが、胸部X 線検査は創傷性心膜炎は否定的であった。病理解剖により慢性化膿性気管支肺炎を生じた肺と心膜が線維性に癒着しており、また病原学的にも肺と心膜の両方からA.pyogenes が分離されており、慢性化膿性気管支肺炎が心膜へ波及して線維素性心膜炎を継発したまれな症例と考えられた。
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3548

トキソブラズマ溶解抗原(TLA)によって誘導される細胞障害性における脾臓の付着性細胞の役割
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: MIYAHARA, Kazuro; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男.
In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with...
Palavras-chave: Adherent cell; Cytotoxic activity; Macrophage; Spleen cell; Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4065

トキソプラズマ溶解抗原(TLA)で誘導されるマウス脾臓内の細胞障害性細胞の性質
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: MIYAHARA, Kazuro; MATSUMOTO, Toru; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男.
Spleen cells from Toxoplasma lysate antigen (TLA)-sensitized BALB/c mice showed the strong cytotoxic activity against both natural killer (NK)-sensitive cells (YAC-1 and RL♂-1) and NK-insensitive cells (P-815), when incubated with TLA or recombinant human IL-2 (rhIL-2). The increment of TLA concentration in culture medium increased the cytotoxic activity. Treatment of effector cells; spleen cells from TLA-sensitized mice incubated with TLA, with anti-asialo GM1 or anti-Thy-1 plus complement inhibited the cytotoxic activity of effector cells, whereas treatment with anti-mouse Lyt-2.2 serum plus complement had no effect on the cytotoxic activity. Treatment of spleen cells from TLA-sensitized mice with anti-asialo GM1 and/or anti-Thy-1 plus complement...
Palavras-chave: Cytotoxic activity; Mouse; Phenotype; Spleen cell; Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4063

Cryopreservation of Canine Ovaries by Vitrification
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安.
The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23...
Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023

ウシの循環器障害に対する臨床診断に関する研究 : ウシの心奇形
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎.
Ano: 1984
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/416

産業動物総合画像診断車の開発とその概要
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎; 佐藤, 基佳; 広瀬, 恒夫; MIYAHARA, Kazuro.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4080

バルーン拡張カテーテルにより緩和的尿路確保を行った前立腺癌の犬の1症例
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 田川, 道人; 倉島, 千尋; 山本, 悠平; 新坊, 弦也; 元尾, 空志; 宮原, 和郎.
11歳7カ月齢のミニチュア・ダックスフンド、去勢オスが血尿を主訴に近医を受診した。対症療法が実施されたものの、排便痛と前立腺腫大が認められ、帯広畜産大学動物医療センターを紹介受診した。X線検査および超音波検査にて前立腺および内腸骨リンパ節を含む複数のリンパ節の腫大が確認され、前立腺尿道部の細胞診にて臨床的に前立腺癌と診断された。ミトキサントロン、ピロキシカム、リン酸トセラニブ等による内科治療を行ったが反応に乏しく、腫瘍浸潤による尿路閉塞が認められた。食道用バルーン拡張カテーテルを用いて緩和的バルーン拡張術を実施し、26日間自力排尿が可能となった。その後急速に転移が進行し、本学受診後第174病日に自宅にて斃死した。
Palavras-chave: 犬; 前立腺癌; バルーン拡張.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4519

Ultrasound Imaging of Mammary Glands in Dairy Heifers at Different Stages of Growth
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: NISHIMURA, Maki; YOSHIDA, Tetsuya; EL-KHODERY, Sabry; MIYOSHI, Masafumi; FURUOKA, Hidefumi; YASUDA, Jun; MIYAHARA, Kazuro; 古岡, 秀文; 宮原, 和郎.
To obtain B-mode ultrasound images of mammary glands in dairy heifers at different stages of growth, 25 clinically normal Holstein heifers were used. The heifers were divided into 5 groups (n=5/group) by stage of their growth: 2-month-old (group 1), 5-month-old (group 2), postpuberty (group 3), mid (group 4), and late (group 5) pregnancy. Furthermore, the sections of mammary glands were observed grossly at postmortem examination in one heifer in each group. Ultrasound images varied with the development of mammary glands. In group 1, the mammary glands had distinctive ultrasonographic findings: an oval to fusiform homogeneous hypoechoic structure. In all groups except group 1, mammary tissue consists of two major areas: a homogeneous, medium echogenic area...
Palavras-chave: Dairy heifer; Mammary gland; Ultrasound.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4068

ホルスタイン種牛における肝臓ならびに後大静脈の画像診断所見と静脈圧との関連について
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 吉林台; 宮原, 和郎; Miyahara, Kazuro.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2210

A comparison of the immunological effects of propofol and isoflurane for maintenance of anesthesia in healthy dogs
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: TOMIHARI, Mizuki; NISHIHARA, Akira; SHIMADA, Terumasa; YANAGAWA, Masashi; MIYOSHI, Masafumi; MIYAHARA, Kazurou; OISHI, Akihiro; 富張, 瑞樹; 宮原, 和郎; 大西, 明弘.
Most anesthetics have an immuno-suppressive effect on cellular and neurohumoral immunity, and research shows that total intravenous anesthesia (TIVA) with propofol has a greater immuno-protective effect than inhalational anesthesia in human medicine. However, in veterinary clinics, these effects remain ambiguous. To clarify the details, we focused on propofol and isoflurane, investigating clinical blood hematology and immunological profiles drawn from healthy dogs under and after two anesthesia techniques. Twelve healthy adult beagles were included in this study, randomly assigned to the propofol anesthesia group (group P: n=6) or the isoflurane anesthesia group (group I: n=6). In both groups, the number of lymphocytes in peripheral blood decreased after 2...
Palavras-chave: Anesthesia; Canine; Immuno-suppression; Isoflurane; Propofol.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4209

三次元超音波検査法による犬胎子の顔の描出
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 三好, 雅史; 西村, 麻紀; 浅井, 寛之; 宮原, 和郎; MIYOSHI, Masafumi; NISHIMURA, Maki; ASAI, Hiroshi; MIYAHARA, Kazuro.
三次元超音波検査法の小動物臨床への応用の可能性を探求するために，妊娠後期の6 歳 のビーグル犬に対して，腹部用三次元プローブを用いた三次元超音波検査法を出産まで数 日毎に行い，8 頭の胎子の顔を描出しようと試みた。胎子の顔の三次元超音波画像が全ての 検査日において少なくとも1 頭の胎子で得られたが，胎子が成長するにつれて良好な画像 を得るのは難しくなった。交配後43 日目ならびに45 日目では胎子の顔の鮮明な三次元画 像が容易に得られた。技術的に解決しなければならない問題がいくつか残されているもの の，適切に用いさえすれば，三次元超音波検査法は従来の二次元超音波検査法を補足する 方法として小動物臨床に応用可能であると考えられる。 This study was delineated to explore the possibility of using three-dimensional ultrasonography in small animal practice. In a 6-year-old pregnant beagle bitch with eight feti, we tried to visualize the feti faces with three-dimensional ultrasonography by scanning with a transabdominal three-dimensional probe every two to three days until parturition. At each date of examination, Three-dimensional sonographic images of the feti faces were...
Palavras-chave: 犬; 顔; 胎子; 三次元超音波検査法; Dog; Face; Fetus; Three-dimensional ultrasonography.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2834

乳牛の乳房炎に対する超音波診断に関する基礎的検討
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎; 西村, 麻紀.
平成22年度 Ⅰ.基盤研究部門
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3376

Methylcholanthrene自家誘発腫瘍担癌ラットに対するトキソプラズマ溶解抗原の抗腫瘍効果
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: MIYAHARA, Kazuro; YOKOO, Naoya; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAKATA, Yu; YOSHIDA, Yutaka; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男.
Growth of the tumor autoinduced by 20-methylcholanthrene (MC) in rats was inhibited after administration of Toxoplasma lysate antigen (TLA). The antitumor activity of TLA was most obvious in the early stage of tumoral growth. When TLA was administered to rats before the appearance of tumor, tumor formation was delayed slightly. Histopathological studies revealed dense growths of spindle tumor cells in untreated control rat, while enlarged central necrosis with the infiltration of lymphocytes and neutrophils was apparent in TLA-treated rats. According to the immunohistological examination of tumor tissue with anti-Thy-1 antibody, the rats treated with TLA showed large Thy-1 positive granular cells, whereas the untreated rats indicated only a few small Thy-1...
Palavras-chave: Antitumor activity; Methylcholanthrene-autoinduced tumor; Rat; Thy-1 positive granular cell; Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4064

乳牛の眼科疾患に対する超音波診断の基礎的検討
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎.
Ano: 1991
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/597

ウシの臨床におけるX線撮影検査の新展開
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4503

第15回国際獣医放射線学会への参加
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 宮原, 和郎.
Palavras-chave: 学会参加; IVRA; 国際獣医放射線学会; ブラジル.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2866

分娩後5カ月以上経過して症状を発現した心室中隔欠損(VSD) の乳午の2例
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 青木, 大介; 滄木, 孝弘; 下田, 崇; 富樫, 義彦; 風間, 武彦; 吉林台; 宮原, 和郎; 古林, 与志安; 古岡, 秀文; 松井, 高峯; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽.
分娩後5か月経過している2歳8カ月齢(症例1)および分娩後6か月経過している3歳0カ月齢(症例2) ホルスタイン種雌乳牛が、食欲不振、泌乳量減少、頻拍、心雑音、頚静脈怒張~拍動等の症状を呈し、循環器の疾患が疑われた。症例lでは心臓超音波検査によりまた症例2では病理検査にて心室中隔欠損が確認された。痩削、頚静脈拍動、頻脈などの循環器症状に加えて、心雑音の聴取をみた場合は、分娩後数カ月を経過した成牛であっても、心内膜炎のほかに心奇形も考慮し、診断には超音波検査を行うことが有効と考えられた。
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2770

ロムスチン抵抗性の犬皮膚型リンパ腫に対して放射線治療を実施した1 例
Provedor de dados: Obihiro University of Agriculture and Veterinary Medicine Academic Repository (OAK)
Autores: 佐藤, 巌紀; 西村, 麻紀; 三好, 雅史; 川本, 恵子; 古林, 与志安; 磯村, 洋; 宮原, 和郎; SATO, Genki; NISHIMURA, Maki; MIYOSHI, Masahumi; KAWAMOTO, Keiko; KOBAYASHI, Yoshiyasu; ISOMURA, Hiroshi; MIYAHARA, Kazurou.
下唇の腫脹と下顎リンパ節の腫脹を伴った1 2 歳のT 細胞型皮膚型リンパ腫のシーズー犬に対してロムスチン （CCNU）による治療を行ったが効果は認められなかった．そこで，1回4Gyを2日おきに計5回，総線量20Gyで放射 線治療を実施したところ，腫脹の軽減が認められた．その後，飼い主希望により，積極的な治療は行わなかったが，数 回の放射線治療のたびに，改善が認められた．最終的に下唇と下顎リンパ節の腫瘤が増大し，第234 病日に斃死した．We administered lomustine (CCNU) to a 12-year-old Shih Tzu with T-cell type cutaneous lymphoma with swelling of the lower lip and mandibular lymph node. The treatment was ineffective and there was no reduction in the swelling in either area. Next, we carried out five radiation treatments (4Gy once every two days) with a total dose of radioactivity of 20Gy. Following the wishes of the owner, we did not perform further treatment. However, after several radiation treatments, there was a noticeable reduction in the swelling of the lower lip and...
Palavras-chave: 皮膚型リンパ腫; ロムスチン（CCNU）; 放射線治療; Cutaneous lymphoma; Lomustine (CCNU); Radiationtherapy.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2940