Consulta: creatorFacets:"Abadie, Eric"
Registros recuperados: 38
Data/hora: 03/06/2024 18:51:18

Two decades of oligotrophication: Evidence for a phytoplankton community shift in the coastal lagoon of Thau (Mediterranean Sea, France)
Provedor de dados: 5
Autores: Derolez, Valerie; Soudant, Dominique; Malet, Nathalie; Chiantella, Claude; Richard, Marion; Abadie, Eric; Aliaume, Catherine; Bec, Beatrice.
Mediterranean costal lagoons have been exposed to anthropic eutrophication for decades. Thau lagoon is one of the largest among them and provides many ecosystem services including shellfish farming. Considerable efforts were made between the 1970s and the late 2000s to improve the wastewater treatment systems in the lagoon watershed. A decrease in nutrient inputs to Thau lagoon was subsequently observed, evidence for the start of a recovery process in Thau lagoon, following oligotrophication. This decrease has significant consequences for ecosystem communities, above all primary producers. In our study, we characterised and quantified long-term changes by analysing long monitoring time-series (1998-2016) of phytoplankton biomass, abundance and composition...
Tipo: Text
Palavras-chave: Eutrophication; Oligotrophication; Nutrients; Recovery; Phytoplankton community shift; Mediterranean coastal lagoon.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00629/74063/73514.pdf

Unsuspected intraspecific variability in the toxin production, growth and morphology of the dinoflagellate Alexandrium pacificum R.W. Litaker (Group IV) blooming in a South Western Mediterranean marine ecosystem, Annaba Bay (Algeria)
Provedor de dados: 5
Autores: Hadjadji, Imen; Laabir, Mohamed; Frihi, Hocine; Collos, Yves; Shao, Zhao Jun; Berrebi, Patrick; Abadie, Eric; Amzil, Zouher; Chomérat, Nicolas; Rolland, Jean-luc; Rieuvilleneuve, Fabien; Masseret, Estelle.
Physiological plasticity gives HABs species the ability to respond to variations in the surrounding environment. The aim of this study was to examine morphological and physiological variability in Alexandrium pacificum R.W. Litaker (Group IV) (former Alexandrium catenella) blooming in Annaba bay, Algeria. Monoclonal cultures of up to 30 strains of this neurotoxic dinoflagellate were established by the germination of single resting cysts from the surface sediment of this southern Mediterranean marine ecosystem. Ribotyping confirmed formally for the first time that A. pacificum is developing in Eastern Algerian waters. Toxin analyses of A. pacificum strains revealed substantial intraspecific variability in both the profile and toxin amount. However, the...
Tipo: Text
Palavras-chave: Alexandrium pacificum; Mediterranean; Toxin profile; Growth; Intraspecific variability.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00624/73620/73598.pdf

Extended evaluation of polymeric and lipophilic sorbents for passive sampling of marine toxins
Provedor de dados: 5
Autores: Zendong, Zita; Herrenknecht, Christine; Abadie, Eric; Brissard, Charline; Tixier, Celine; Mondeguer, Florence; Sechet, Veronique; Amzil, Zouher; Hess, Philipp.
Marine biotoxins are algal metabolites that can accumulate in fish or shellfish and render these foodstuffs unfit for human consumption. These toxins, released into seawater during algal occurrences, can be monitored through passive sampling. Acetone, methanol and isopropanol were evaluated for their efficiency in extracting toxins from algal biomass. Isopropanol was chosen for further experiments thanks to a slightly higher recovery and no artifact formation. Comparison of Oasis HLB, Strata-X, BondElut C18 and HP-20 sorbent materials in SPE-mode led to the choice of Oasis HLB, HP-20 and Strata-X. These three sorbents were separately exposed as passive samplers for 24 h to seawater spiked with algal extracts containing known amounts of okadaic acid (OA),...
Tipo: Text
Palavras-chave: Passive sampling; Oasis HLB; Strata-X; LOPE; PDMS; SPATT.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00186/29756/28187.pdf

Pinnatoxins’ Deleterious Effects on Cholinergic Networks: From Experimental Models to Human Health
Provedor de dados: 5
Autores: Delcourt, Nicolas; Lagrange, Emmeline; Abadie, Eric; Fessard, Valérie; Frémy, Jean-marc; Vernoux, Jean-paul; Peyrat, Marie-bénédicte; Maignien, Thomas; Arnich, Nathalie; Molgó, Jordi; Mattei, César.
Pinnatoxins (PnTXs) are emerging neurotoxins that were discovered about 30 years ago. They are solely produced by the marine dinoflagellate Vulcanodinium rugosum, and may be transferred into the food chain, as they have been found in various marine invertebrates, including bivalves. No human intoxication has been reported to date although acute toxicity was induced by PnTxs in rodents. LD50 values have been estimated for the different PnTXs through the oral route. At sublethal doses, all symptoms are reversible, and no neurological sequelae are visible. These symptoms are consistent with impairment of central and peripheral cholinergic network functions. In fact, PnTXs are high-affinity competitive antagonists of nicotinic acetylcholine receptors (nAChRs)....
Tipo: Text
Palavras-chave: Pinnatoxins; Cyclic imines; Vulcanodinium rugosum; Nicotinic acetylcholine receptors; Acute neurotoxicity; Human intoxication; Myasthenia gravis.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00507/61883/65958.pdf

A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella
Provedor de dados: 5
Autores: Rolland, Jean-luc; Medhioub, Walid; Vergnes, Agnes; Abi-khalil, Celina; Savar, Veronique; Abadie, Eric; Masseret, Estelle; Amzil, Zouher; Laabir, Mohamed.
To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in...
Tipo: Text
Palavras-chave: Shellfish; Toxin; Biomarker; Expression; Phytoplankton.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00225/33606/32003.pdf

A comparative analysis of Alexandrium catenella/tamarense blooms in Annaba Bay (Algeria) and Thau lagoon (France); phosphorus limitation as a trigger
Provedor de dados: 5
Autores: Hadjadji, Imene; Frehi, Hocine; Ayada, Lembarek; Abadie, Eric; Collos, Yves.
Environmental conditions ultimately leading to blooms of the toxic dinoflagellate Alexandrium catenella/tamarense were investigated at two Mediterranean sites (Annaba Bay, Algeria and Thau lagoon, France). Three years were examined in details: 1992 (a pre-Alexandrium period), 2002 (a year with the first bloom in Annaba) and 2010 (a year with a major bloom in Annaba). Most conditions were similar, but ammonium concentrations were much higher in Annaba (up to 100 μM) than in Thau (up to 10 μM). First records of A. catenella/tamarense were in 1995 for Thau and 2002 for Annaba, and coincided with soluble reactive phosphorus (SRP) decreasing below a concentration of about 1 μM. No other environmental variable could be related to those blooms. Thus, it is likely...
Tipo: Text
Palavras-chave: Alexandrium catenella/tamarense; Blooms; Algeria; France; Mediterranean.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00174/28574/26995.pdf

REPHYTOX Leucate. Mise en forme des résultats sur la période 2014-2020
Provedor de dados: 5
Autores: Serais, Ophelie; Abadie, Eric; Hubert, Clarisse; Bellamy, Elise; Fiandrino, Annie; Lemoine, Maud; Neaud-masson, Nadine.
Ce rapport a été réalisé à la demande de la Direction Départementale de la Protection des Populations de l’Hérault (DDPP34). Les résultats en toxines lipophiles réglementées (Acide Okadaïque, Dinophysis toxines et Pectenotoxines uniquement) dans les moules et les huîtres prélevées au point « Parc Leucate 2 » dans le cadre du REPHYTOX, ainsi que les dénombrements du phytoplancton toxique du genre Dinophysis réalisés dans le cadre du REPHY dans la lagune de Leucate, sont présentés sur des graphiques permettant une visualisation temporelle des épisodes de contamination entre janvier 2014 et décembre 2020.
Tipo: Text
Palavras-chave: REPHYTOX; REPHY; Leucate; Point Parc Leucate 2; Moules; Huîtres; Toxins lipophiles; Dinophysis; Graphiques; Période 2014-2020.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00662/77444/79099.pdf

Report on the first detection of pectenotoxin-2, spirolide-A and their derivatives in French shellfish
Provedor de dados: 5
Autores: Amzil, Zouher; Sibat, Manoella; Royer, Florence; Masson, Nadine; Abadie, Eric.
In the context of the French Phytoplankton and Phycotoxins Monitoring Network (REPHY) programme, shellfish samples were harvested from different locations where harmful algae blooms were known to have occurred. For all shellfish samples found positive by the mouse bioassay for diarrhetic shellfish poisoning (DSP) toxins, liquid chromatography (LC) coupled with mass spectrometry (MS) was used to search for the following lipophilic toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines (GYMs). In order to investigate the presence of acyl-OAs and/or acyl-DTX-1,-2 (DTX-3), alkaline hydrolysis was performed on all samples, and LC/ MS analyses were carried out on the...
Tipo: Text
Palavras-chave: LC/MS analysis; Pectenotoxins; Spirolides; DSP toxins.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-6206.pdf

Contribution of several nitrogen sources to growth of Alexandrium catenella during blooms in Thau lagoon, southern France
Provedor de dados: 5
Autores: Collos, Y; Vaquer, A; Laabir, M; Abadie, Eric; Laugier, Thierry; Pastoureaud, Annie; Souchu, Philippe.
A monitoring program with a weekly sampling frequency over a 15-month period indicates that urea concentrations above a certain threshold level may trigger the blooms of Alexandrium catenella in Thau lagoon. However, urea concentrations were also sometimes related to ammonium and dissolved organic nitrogen concentrations, indicating that the role of urea may not be a direct one. An original approach is used to assess the relative contribution of several nitrogen sources (nitrate, nitrite, ammonium, urea) to growth of A. catenella by comparing nitrogen uptake rates to nitrogen-based growth rates estimated from dilution experiments during four blooms over a 4-year period (2001-2004) in Thau lagoon. Nitrate and nitrite contributed 0.1-14% and 0.1-5%...
Tipo: Text
Palavras-chave: Uptake kinetics; Ammonium; Urea; Growth; Nitrogen; HAB; Alexandrium catenella.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-6454.pdf

Health risk assessment related to pinnatoxins in French shellfish
Provedor de dados: 5
Autores: Arnich, Nathalie; Abadie, Eric; Delcourt, Nicolas; Fessard, Valérie; Fremy, Jean-marc; Hort, Vincent; Lagrange, Emmeline; Maignien, Thomas; Molgó, Jordi; Peyrat, Marie-bénédicte; Vernoux, Jean-paul; Mattei, César.
Pinnatoxins (PnTXs) are a group of emerging marine biotoxins produced by the benthic dinoflagellate Vulcanodinium rugosum, currently not regulated in Europe or in any other country in the world. In France, PnTXs were detected for the first time in 2011, in mussels from the Ingril lagoon (South of France, Mediterranean coast). Since then, analyses carried out in mussels from this lagoon have shown high concentrations of PnTXs for several months each year. PnTXs have also been detected, to a lesser extent, in mussels from other Mediterranean lagoons and on the Atlantic and Corsican coasts. In the French data, the main analog is PnTX G (low levels of PnTX A are also present in some samples). No cases of PnTXs poisoning in humans have been reported so far in...
Tipo: Text
Palavras-chave: Pinnatoxins; Shellfish; Emerging marine biotoxins; Risk assessment.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00619/73105/72236.pdf

Etude de Vulcanodinium rugosum (Dinoflagellé producteur de pinnatoxines) se développant dans la lagune méditerranéenne de l’Ingril
Provedor de dados: 5
Autores: Abadie, Eric.
Harmful and / or toxic phytoplankton blooms impact for a long time marine ecosystems worldwide. These massive developments have an adverse effect on ecosystems and their exploitation. The lagoons of the region Languedoc-Roussillon are affected for decades. In addition to recurring health risks from Dinophysis (DSP toxins) and Alexandrium (PSP toxins), Vulcanodinium rugosum was identified in 2011 in the lagoon Ingril. This new species product pinnatoxins (neurotoxins). Through this thesis work, the biology of this new species (life cycle, growth condition, toxin production), its shellfish contamination capacity and geographic distribution in neighboring lagoons were studied on cultures in the laboratory and via environmental monitoring. The results of this...
Tipo: Text
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00473/58425/61002.pdf

Evaluation de la qualité des zones de production conchylicole.Départements des Pyrénées orientales, de l’Aude, de l’Hérault et du Gard. Edition 2018
Provedor de dados: 5
Autores: Crottier, Anais; Abadie, Eric; Brun, Melanie; Piquet, Jean-come; Fiandrino, Annie.
Après un rappel des objectifs, du fonctionnement et de la méthode d’interprétation des résultats des réseaux de contrôle microbiologique (REMI) et de surveillance chimique (ROCCH), ce rapport inclut un bilan national et décrit le programme annuel des départements de l’Hérault, du Gard et de l’Aude. Il présente l’ensemble des résultats obtenus, en particulier l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées pour la période 2015-2017. Sur les 29 zones de production conchylicole classées et suivies en Occitanie, la qualité a pu être estimée pour 23 d’entre elles. Selon les critères réglementaires pris en compte (règlement CE n°854/2004, modifié par le règlement n°2285/2015), dans les départements de...
Tipo: Text
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Classement sanitaire des zones de production; Département de l’Hérault; Départemen tdu Gard; Département de l’Aude.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00445/55635/57280.pdf

Etude sanitaire de la zone n° 34.39 "Lotissements conchylicoles de la lagune de Thau" Groupe III. Département de l'Hérault
Provedor de dados: 5
Autores: Marzin, Anahita; Crottier, Anais; Piquet, Jean-come; Abadie, Eric; Roque D'Orbcastel, Emmanuelle; Fiandrino, Annie.
L’étude de zone des lotissements conchylicoles de la lagune de Thau (34.39) a été menée, à la demande du Comité Régional de la Conchyliculture en Méditerranée et du préfet de l’Hérault, pour étudier la possibilité de sectoriser la zone de production en sous-zones, au regard du risque microbiologiques (REMI). Une étude de dossier a été réalisée afin de recenser les principales sources de contamination. Celles-ci sont essentiellement d’origine anthropique et impactent différemment les secteurs envisagés. Des prélèvements de coquillages du groupe 3 ont été réalisés de mai 2017 à mai 2018 avec une fréquence bimensuelle. Au regard des critères du règlement (CE) n° 854/2004, les résultats des analyses microbiologiques (indicateur Escherichia coli) ont permis...
Tipo: Text
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Étude de zone; Lagune de Thau; Département de l’Hérault.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00459/57056/58965.pdf

What are the main environmental factors driving the development of the neurotoxic dinoflagellate Vulcanodinium rugosum in a Mediterranean ecosystem (Ingril lagoon, France)?
Provedor de dados: 5
Autores: Abadie, Eric; Chiantella, Claude; Crottier, Anais; Rhodes, Lesley; Masseret, Estelle; Berteaux, Tom; Laabir, Mohamed.
Vulcanodinium rugosum, a dinoflagellate developing in Ingril Lagoon (Mediterranean, France) is responsible for shellfish intoxications due to the neurotoxin pinnatoxin G. A one year survey (March 2012–April 2013) was conducted in this oligotrophic shallow lagoon and key environmental parameters were recorded (temperature, salinity and nutrients). The spatio-temporal distribution of V. rugosum in water column and on macrophytes was also determined. Planktonic cells of V. rugosum were observed at all sampling stations, but in relatively low concentrations (maximum of 1000 cell/L). The highest abundances were observed from June to September 2012. There was a positive correlation between cell densities and both temperature and salinity. Non-motile cells were...
Tipo: Text
Palavras-chave: Vulcanodinium rugosum; Neurotoxins; Ingril lagoon; Environmental factors; Development dynamic.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00437/54862/56478.pdf

La crise anoxique du bassin de Thau de l'été 1997. Bilan du phénomène et perspectives
Provedor de dados: 5
Autores: Souchu, Philippe; Abadie, Eric; Vercelli, Catherine; Buestel, Dominique; Sauvagnargues, Jean-claude.
La crise anoxique ou «malaïgue» qui a sévi dans le bassin de Thau durant l'été 1997 a détruit presque le tiers de la production annuelle d'huîtres. Son déclenchement a suivi une période durant laquelle les vents étaient exceptionnellement faibles. Après un rappel scientifique des processus mis en jeu, la crise anoxique est décrite au travers des observations de mortalités et des concentrations en oxygène dissous. Les eaux anoxiques se sont développées dans les zones conchylicoles vers l'Est et l'Ouest à partir d'un foyer situé à proximité du principal port conchylicole (Mourre-Blanc à Mèze). La comparaison de la crise de 1997 avec celles qui l'ont précédée fait apparaître un recul de l'eutrophisation depuis 25 ans. En revanche, des secteurs très localisés...
Tipo: Text
Palavras-chave: Lagunes; Thau; Conchyliculture; Anoxies; Eutrophisation; Crassostrea gigas.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00039/15010/12336.pdf

Influence of Environmental Factors on the Paralytic Shellfish Toxin Content and Profile of Alexandrium catenella (Dinophyceae) Isolated from the Mediterranean Sea
Provedor de dados: 5
Autores: Laabir, Mohamed; Collos, Yves; Masseret, Estelle; Grzebyk, Daniel; Abadie, Eric; Savar, Veronique; Sibat, Manoella; Amzil, Zouher.
Laboratory experiments were designed to study the toxin content and profile of the Alexandrium catenella strain ACT03 (isolated from Thau Lagoon, French Mediterranean) in response to abiotic environmental factors under nutrient-replete conditions. This dinoflagellate can produce various paralytic shellfish toxins with concentrations ranging from 2.9 to 50.3 fmol/cell. The toxin profile was characterized by carbamate toxins (GTX3, GTX4 and GTX5) and N-sulfocarbamoyl toxins (C1, C2, C3 and C4). C2 dominated at 12-18 degrees C, but only for salinities ranging from 10 to 25 psu, whereas GTX5 became dominant at temperatures ranging from 21 to 30 degrees C at almost all salinities. There was no significant variation in the cellular toxin amount from 18 degrees C...
Tipo: Text
Palavras-chave: Alexandrium catenella; PSP toxins; Temperature; Salinity; Light.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00148/25969/24056.pdf

Contamination de l'etang de Thau par Alexandrium tamarense. Episode de novembre a decembre 1998
Provedor de dados: 5
Autores: Abadie, Eric; Zouher, Amzil; Belin, Catherine; Comps, Marie-annick; Elziere-papayanni, Panayota; Lassus, Patrick; Le Bec, Claude; Marcaillou-le Baut, Claire; Nezan, Elizabeth; Poggi, Robert.
A bloom of the toxic dinoflagellate Alexandrium tamarense was observed for the first time in Thau iagoon between November and December 1998. Maximum cell concentrations detected in shellfish farming areas (85 000 Cells/1) were high enough to make mussels and clams unsuitable for human consumption (max. 850 ug. STX eq.100 g'1) while toxic levels in oysters did not exceed, or even reach, the international public health threshold established for PSP toxins.  This event, new to French Mediterranean waters, was investigated by both local and national veterinary services and by the Ifremer teams involved in the National Monitoring Network for Phytoplankton and Phycotoxines (Réphy). The chronological and operational description of the event was compared with data...
Tipo: Text
Palavras-chave: Alexandrium; Monitoring; Toxic dinoflagellates; Thau lagoon; Alexandrium; Surveillance; Dinoflagellés toxiques; Etang de Thau.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/rapport-884.pdf

Dietary BMAA Exposure in an Amyotrophic Lateral Sclerosis Cluster from Southern France
Provedor de dados: 5
Autores: Masseret, Estelle; Banack, Sandra; Boumediene, Farid; Abadie, Eric; Brient, Luc; Pernet, Fabrice; Juntas-morales, Raoul; Pageot, Nicolas; Metcalf, James; Cox, Paul; Camu, William.
Background  Dietary exposure to the cyanotoxin BMAA is suspected to be the cause of amyotrophic lateral sclerosis in the Western Pacific Islands. In Europe and North America, this toxin has been identified in the marine environment of amyotrophic lateral sclerosis clusters but, to date, only few dietary exposures have been described.  Objectives  We aimed at identifying cluster(s) of amyotrophic lateral sclerosis in the Hérault district, a coastal district from Southern France, and to search, in the identified area(s), for the existence of a potential dietary source of BMAA.  Methods  A spatio-temporal cluster analysis was performed in the district, considering all incident amyotrophic lateral sclerosis cases identified from 1994 to 2009 by our expert...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00168/27941/26234.pdf

Oyster Farming, Temperature, and Plankton Influence the Dynamics of Pathogenic Vibrios in the Thau Lagoon
Provedor de dados: 5
Autores: Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine.
Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French...
Tipo: Text
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf

Searching for a link between the L-BMAA neurotoxin and amyotrophic lateral sclerosis: a study protocol of the French BMAALS programme
Provedor de dados: 5
Autores: Delzor, Aurelie; Couratier, Philippe; Boumediene, Farid; Nicol, Marie; Druet-cabanac, Michel; Paraf, Francois; Mejean, Annick; Ploux, Olivier; Leleu, Jean-philippe; Brient, Luc; Lengronne, Marion; Pichon, Valerie; Combes, Audrey; El Abdellaoui, Saida; Bonneterre, Vincent; Lagrange, Emmeline; Besson, Gerard; Bicout, Dominique J.; Boutonnat, Jean; Camu, William; Pageot, Nicolas; Juntas-morales, Raul; Rigau, Valerie; Masseret, Estelle; Abadie, Eric; Preux, Pierre-marie; Marin, Benoit.
Introduction: Amyotrophic lateral sclerosis (ALS) is the most common motor neurone disease. It occurs in two forms: (1) familial cases, for which several genes have been identified and (2) sporadic cases, for which various hypotheses have been formulated. Notably, the beta-N-methylamino-L-alanine (L-BMAA) toxin has been postulated to be involved in the occurrence of sporadic ALS. The objective of the French BMAALS programme is to study the putative link between L-BMAA and ALS. Methods and analysis: The programme covers the period from 1 January 2003 to 31 December 2011. Using multiple sources of ascertainment, all the incident ALS cases diagnosed during this period in the area under study (10 counties spread over three French regions) were collected....
Tipo: Text
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00443/55431/56952.pdf

Exposure to the Paralytic Shellfish Toxin Producer Alexandrium catenella Increases the Susceptibility of the Oyster Crassostrea gigas to Pathogenic Vibrios
Provedor de dados: 5
Autores: Abi-khalil, Celina; Lopez-joven, Carmen; Abadie, Eric; Savar, Veronique; Amzil, Zouher; Laabir, Mohamed; Rolland, Jean-luc.
The multifactorial etiology of massive Crassostrea gigas summer mortalities results from complex interactions between oysters, opportunistic pathogens and environmental factors. In a field survey conducted in 2014 in the Mediterranean Thau Lagoon (France), we evidenced that the development of the toxic dinoflagellate Alexandrium catenella, which produces paralytic shellfish toxins (PSTs), was concomitant with the accumulation of PSTs in oyster flesh and the occurrence of C. gigas mortalities. In order to investigate the possible role of toxic algae in this complex disease, we experimentally infected C. gigas oyster juveniles with Vibrio tasmaniensis strain LGP32, a strain associated with oyster summer mortalities, after oysters were exposed to Alexandrium...
Tipo: Text
Palavras-chave: Harmful algae; Environment; Interaction; Pathogens; Defense; Paralytic shellfish toxin.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00309/42016/41308.pdf

Contamination virale de l'environnement littoral
Provedor de dados: 5
Autores: Miossec, Laurence; Besseau, Laetitia; Haugarreau, Larissa; Le Guyader, Francoise; Menard, Dominique; Pommepuy, Monique; Abadie, Eric; Comps, Marie-annick; Guillou, Jean-louis; Le Bec, Claude; Sauvagnargues, Jean-claude; Bon, Fabienne; Jamot, B.; Kohli E, Evelyne; Potier, P.; Daurat, G.; Guyonnet, J.p.
Les microorganismes, bactéries et virus d'origine entérique, sont abondants dans les rejets urbains. Si la charge bactériologique contenue dans les eaux usées est notablement réduite lors des traitements appliqués dans les stations d'épurations, de nombreux microorganismes persistent et sont rejetés avec les eaux épurées dans le milieu récepteur, en rivière ou directement en mer. Le développement de méthodes analytiques basées sur la biologie moléculaire a permis récemment la détection de virus entériques non cultivables dans les échantillons de l'environnement, tels que les entérovirus, le virus de l'hépatite A, les calicivirus, les astrovirus et les rotavirus. En France, les données publiées sur la présence de ces virus entériques, détectés par RTPCR,...
Tipo: Text
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00044/15552/12941.pdf

β-N-methylamino-l-alanine (BMAA) and isomers: Distribution in different food web compartments of Thau lagoon, French Mediterranean Sea
Provedor de dados: 5
Autores: Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Masseret, Estelle; Hess, Philipp; Amzil, Zouher.
The neurotoxin BMAA (β-N-methylamino-l-alanine) and its isomer DAB (2,4-diaminobutyric acid) have been detected in seafood worldwide, including in Thau lagoon (French Mediterranean Sea). A cluster of amyotrophic lateral sclerosis (ALS), a neurodegenerative disease associated with BMAA, has also been observed in this region. Mussels, periphyton (i.e. biofilms attached to mussels) and plankton were sampled between July 2013 and October 2014, and analyzed using HILIC-MS/MS. BMAA, DAB and AEG (N-(2-aminoethyl)glycine) were found in almost all the samples of the lagoon. BMAA and DAB were present at 0.58 and 0.83, 2.6 and 3.3, 4.0 and 7.2 μg g−1 dry weight in plankton collected with nets, periphyton and mussels, respectively. Synechococcus sp., Ostreococcus...
Tipo: Text
Palavras-chave: BMAA; Diatoms; Chaetoceros sp.; Seston; Mytilus galloprovincialis; HILIC-MS/MS.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00275/38607/37192.pdf

Effect of Nitrate, Ammonium and Urea on Growth and Pinnatoxin G Production of Vulcanodinium rugosum
Provedor de dados: 5
Autores: Abadie, Eric; Kaci, Lamia; Berteaux, Tom; Hess, Philipp; Sechet, Veronique; Masseret, Estelle; Rolland, Jean-luc; Laabir, Mohamed.
ulcanodinium rugosum, a recently described dinoflagellate species producing a potent neurotoxin (pinnatoxin G), has been identified in French Mediterranean lagoons and was responsible for recurrent episodes of shellfish toxicity detected by mouse bioassay. Until now, the biology and physiology of V. rugosum have not been fully investigated. We studied the growth characteristics and toxicity of a V. rugosum strain (IFR-VRU-01), isolated in the Ingril lagoon in June 2009 (North-Western French Mediterranean Sea). It was cultivated in Enriched Natural Sea Water (ENSW) with organic (urea) and inorganic (ammonium and nitrate) nitrogen, at a temperature of 25 °C and irradiance of 100 μmol/m2·s−1. Results showed that ammonium was assimilated by cells more rapidly...
Tipo: Text
Palavras-chave: Vulcanodinium rugosum; Pinnatoxin G; Ingril lagoon; Growth; Nitrogen source.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00276/38748/37290.pdf

Pinnatoxines en lien avec l’espèce Vulcanodinium rugosum (II)
Provedor de dados: 5
Autores: Mondeguer, Florence; Abadie, Eric; Herve, Fabienne; Bardouil, Michele; Sechet, Veronique; Raimbault, Virginie; Berteaux, Tom; Zendong, Suzie Zita; Palvadeau, Hubert; Amzil, Zouher; Hess, Philipp; Fessard, Valérie; Huguet, Antoine; Sosa, Silvio; Tubaro, Aurelia; Aráoz, Rómulo; Molgó, Jordi.
Pour tenter d’apporter des réponses aux questions soulevées par une précédente étude commanditée pour approfondir les connaissances sur Vulcanodinium rugosum, l’organisme producteur de la Pinnatoxine G (PnTX G) (Hess et al., 2012), les objectifs de cette nouvelle étude sont les suivants : - Elargissement de la zone initiale de prélèvement d’Ingril à toute la lagune méditerranéenne et mise à profit de l’augmentation du nombre d’échantillons pour vérifier l’impact de l’effet matrice dans l’analyse LC-SM/MS. - Mise en culture en masse de Vulcanodinium rugosum et - Conduite d’une expérience de contamination in vivo sur des moules pour vérifier l’accumulation de la PnTX-G et comprendre les mécanismes de la métabolisation des PnTXs et de ses composés associés.....
Tipo: Text
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00285/39635/38126.pdf

Stratégie d’échantillonnage des lieux du REPHY Sanitaire et REPHYTOX pour la région Occitanie
Provedor de dados: 5
Autores: Serais, Ophelie; Caillard, Elise; Abadie, Eric; Fiandrino, Annie.
Cette procédure a pour objet de décrire la stratégie d’échantillonnage des lieux de surveillance du REPHY Sanitaire et REPHYTOX dans la région Occitanie. Les logigrammes, présentés par secteur géographique, indiquent la logique opérationnelle à suivre entre les lieux de surveillance du REPHY et du REPHYTOX dans le cadre de la mise en oeuvre des stratégies de ces réseaux. Ces stratégies sont détaillées dans le document de procédure nationale REPHYTOX (version en vigueur) et plus particulièrement dans le paragraphe « STRATEGIE D'ECHANTILLONNAGE ».
Tipo: Text
Palavras-chave: REPHY; REPHYTOX; Réseaux de surveillance; Toxines; Logigrammes; Occitanie.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00676/78856/81979.pdf

Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae)
Provedor de dados: 5
Autores: Laporte, Martin; Shao, Zhaojun; Berrebi, Patrick; Laabir, Mohamed; Abadie, Eric; Faivre, Nicolas; Rieuvilleneuve, Fabien; Masseret, Estelle.
Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic...
Tipo: Text
Palavras-chave: Invasive species; Phytoplankton; Alexandrium; Dinophyceae; Microsatellite markers; Biogeography.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00189/30026/29398.pdf

Pinnatoxines en lien avec l’espèce Vulcanodinium rugosum
Provedor de dados: 5
Autores: Hess, Philipp; Herve, Fabienne; Abadie, Eric; Sechet, Veronique; Molgo, Jordi; Amzil, Zouher; Fessard, Valerie.
Suite à la découverte de la Pinnatoxine G comme agent responsable des toxicités atypiques à Ingril fin 2010, une étude a été commanditée afin d’approfondir les connaissances sur l’organisme producteur de cette toxine, Vulcanodinium rugosum, les toxines associées et leur toxicité.   L’organisme, étant considéré « cryptique » dû à sa faible présence dans le milieu, a été suivi de manière plus proche à Ingril. Il est apparu dans la colonne d’eau en juillet et août 2012, et quelques échantillons ont pu être prélevés pour la mise en culture de souches supplémentaires. Une corrélation directe entre la croissance de l’organisme et la température est soupçonnée mais n’a pas encore pu être corroborée sur la période d’étude, faute d’un nombre suffisant...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00094/20518/18190.pdf

The spatio-temporal evolution of the genus Nitzschia Longissima at the level of the lagoon in Nador, Morocco
Provedor de dados: 5
Autores: Riouchi, Ouassila; El Madani, Faid; Abadie, Eric; Skalli, Ali; Baghour, Mourad.
This work aims to study the spatio-temporal evolution of the genus Nitzschia longissima, one of the most important genera of marine plankton diatoms, from 3 sampling stations in the Nador lagoon and during 2 seasons (spring and summer 2018), Using Nitzschia longissima, as a study system, one of the most diverse and abundant genera among marine planktonic diatoms. This species counts, in addition to the form Nitzschia longissima forma parva Grunow, three varieties namely Nitzschia longissima var. closterium (W. Smith) Van Heurck, Nitzschia longissima var. longissima (Breb.) Ralfs and Nitzschia longissima var. reversa Grunow. Nitzschia Longissima genus density was high during the warm season (Summer 2018) with a value of 8000 cells/liter, and low during the...
Tipo: Text
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00681/79354/81860.pdf

Pinnatoxin G is responsible for atypical toxicity in mussels (Mytilus galloprovincialis) and clams (Venerupis decussata) from Ingril, a French Mediterranean lagoon
Provedor de dados: 5
Autores: Hess, Philipp; Abadie, Eric; Herve, Fabienne; Berteaux, Tom; Sechet, Veronique; Araoz, Romulo; Molgo, Jordi; Zakarian, Armen; Sibat, Manoella; Rundberget, Thomas; Miles, Christopher O.; Amzil, Zouher.
Following a review of official control data on shellfish in France, Ingril Lagoon had been identified as a site where positive mouse bioassays for lipophilic toxins had been repeatedly observed. These unexplained mouse bioassays, also called atypical toxicity, coincided with an absence of regulated toxins and rapid death times in mice observed in the assay.  The present study describes pinnatoxin G as the main compound responsible for the toxicity observed using the mouse bioassay for lipophilic toxins. Using a well-characterised standard for pinnatoxin G, LC-MS/MS analysis of mussel samples collected from 2009 to 2012 revealed regular occurrences of pinnatoxin G at levels sufficient to account for the toxicity in the mouse bioassays. Baseline levels of...
Tipo: Text
Palavras-chave: Cyclic imines; Shellfish toxin; Accumulation; Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS); Unexplained mouse toxicity.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00158/26960/25255.pdf

Oligotrophication and emergence of picocyanobacteria and a toxic dinoflagellate in Thau lagoon, southern France
Provedor de dados: 5
Autores: Collos, Yves; Bec, Beatrice; Jauzein, Cecile; Abadie, Eric; Laugier, Thierry; Lautier, Jacques; Pastoureaud, Annie; Souchu, Philippe; Vaquer, Andre.
Time series data have been examined in Thau lagoon (Southern France) from 1972 to 2006 for water temperature, salinity, nutrients and from 1987 to 2006 for phytoplankton. A first main trend identified is an increase in mean annual water temperature (1.5 degrees C over 33 years or 0.045 degrees C/year) that was not evenly distributed among seasons. The highest rate of increase was in the spring (+ 3.0 degrees C over 33 years), followed by summer (+ 2.0 degrees C and fall (+ 1.7 degrees C. In winter, no significant increase over the 33 year period could be found. A second clear trend is a large decrease in soluble reactive phosphorus (SRP) concentration over the same 33 year period (summer values decreased from 10 mu M to 1 mu M. while winter values...
Tipo: Text
Palavras-chave: Skeletonema costatum; Alexandrium catenella; Picocyanobacteria; Oligotrophication; Long term variability; Thau lagoon.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6420.pdf

Toxin and Growth Responses of the Neurotoxic Dinoflagellate Vulcanodinium rugosum to Varying Temperature and Salinity
Provedor de dados: 5
Autores: Abadie, Eric; Muguet, Alexia; Berteaux, Tom; Chomerat, Nicolas; Hess, Philipp; Roque D'Orbcastel, Emmanuelle; Masseret, Estelle; Laabir, Mohamed.
Vulcanodinium rugosum, a recently described species, produces pinnatoxins. The IFR-VRU-01 strain, isolated from a French Mediterranean lagoon in 2010 and identified as the causative dinoflagellate contaminating mussels in the Ingril Lagoon (French Mediterranean) with pinnatoxin-G, was grown in an enriched natural seawater medium. We tested the effect of temperature and salinity on growth, pinnatoxin-G production and chlorophyll a levels of this dinoflagellate. These factors were tested in combinations of five temperatures (15, 20, 25, 30 and 35 °C) and five salinities (20, 25, 30, 35 and 40) at an irradiance of 100 µmol photon m−2 s−1. V. rugosum can grow at temperatures and salinities ranging from 20 °C to 30 °C and 20 to 40, respectively. The optimal...
Tipo: Text
Palavras-chave: Vulcanodinium rugosum; Mediterranean Ingril Lagoon; Toxin production; Growth conditions; Temperature; Salinity.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00333/44429/44095.pdf

Troubles neurologiques liés à la consommation de coquillages : des intoxications au diagnostic encore peu connu
Provedor de dados: 5
Autores: Sinno-tellier, Sandra; Delcourt, Nicolas; Abadie, Eric.
La consommation de coquillages bivalves (moules, huîtres...) peut être à l’origine de troubles neurologiques graves voire mortels, liés à l’accumulation dans la chair des mollusques de toxines naturelles dont les teneurs sont par ailleurs réglementées. En juin 2019, une intoxication spécifique liée à la consommation de moules contaminées par des toxines paralysantes a attiré l’attention de l’Anses et des Centres antipoison (CAP) sur une possible méconnaissance diagnostique des intoxications par des neurotoxines marines. Une étude rétrospective des cas d’intoxication par des coquillages enregistrés par les CAP de 2012 à 2019, et des données de surveillance réglementaire des zones de production conchylicoles, a permis d’identifier 15 cas probables...
Tipo: Text
Palavras-chave: Sécurité des aliments; Neurotoxines; Intoxication; Centres antipoison; Coquillages; Conchyliculture.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00691/80279/83364.pdf

Response of diatom and dinoflagellate lifeforms to reduced phosphorus loading: a case study in the Thau lagoon, France
Provedor de dados: 5
Autores: Gowen, R. J.; Collos, Y.; Tett, P.; Scherer, C.; Bec, B.; Abadie, Eric; Allen, M.; O'Brien, T.
The basin of Thau in southern France is a shallow, weakly flushed lagoon which is an important location for oyster cultivation. Phytoplankton analyses were carried out in 1975-1976 and then (almost) continuously since 1987. We report an investigation of 'the balance of organisms' in phytoplankton in relation to reductions in phosphorus loading, using two new tools based on phytoplankton lifeforms: the Plankton Index for Phytoplankton (PIp); Euclidean distance in state space. Our results show the utility of the tools for analysing changes in the ‘balance of organisms’ at the level of functional groups (in our study diatoms and dinoflagellates), but also illustrate the difficulties in demonstrating the reversal of human impacts resulting from eutrophication....
Tipo: Text
Palavras-chave: Eutrophication; Thau lagoon; Phosphorus; Lifeform; State-space; Euclidean distance.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00257/36817/35449.pdf

Toxicity and Growth Assessments of Three Thermophilic Benthic Dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) Developing in the Southern Mediterranean Basin
Provedor de dados: 5
Autores: Ben-gharbia, Hela; Yahia, Ons Kefi-daly; Amzil, Zouher; Chomerat, Nicolas; Abadie, Eric; Masseret, Estelle; Sibat, Manoella; Triki, Habiba Zmerli; Nouri, Habiba; Laabir, Mohamed.
armful benthic dinoflagellates, usually developing in tropical areas, are expanding to temperate ecosystems facing water warming. Reports on harmful benthic species are particularly scarce in the Southern Mediterranean Sea. For the first time, three thermophilic benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) were isolated from Bizerte Bay (Tunisia, Mediterranean) and monoclonal cultures established. The ribotyping confirmed the morphological identification of the three species. Maximum growth rates were 0.59 ± 0.08 d−1 for O. cf. ovata, 0.35 ± 0.01 d−1 for C. monotis and 0.33 ± 0.04 d−1 for P. lima. Toxin analyses revealed the presence of ovatoxin-a and ovatoxin-b in O. cf. ovata cells. Okadaic acid and...
Tipo: Text
Palavras-chave: Ostreopsis cf. ovata; Prorocentrum lima; Coolia monotis; Southern Mediterranean Sea; Growth; Toxicity.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00355/46585/46393.pdf

Beta-N-Methylamino-L-Alanine: LC-MS/MS Optimization, Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau, a French Mediterranean Lagoon
Provedor de dados: 5
Autores: Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Brient, Luc; Savar, Veronique; Bardouil, Michele; Hess, Philipp; Amzil, Zouher.
beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral...
Tipo: Text
Palavras-chave: Cyanotoxins; BMAA; DAB; AEG; HILIC-MS/MS; Cyanobacteria; Bivalve mollusks; French Mediterranean.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00245/35579/34112.pdf

Comparing diatom and Alexandrium catenella/tamarense blooms in Thau lagoon: Importance of dissolved organic nitrogen in seasonally N-limited systems
Provedor de dados: 5
Autores: Collos, Yves; Jauzein, Cecile; Ratmaya, Widya; Souchu, Philippe; Abadie, Eric; Vaquer, André.
Diatom blooms in Thau lagoon are always related to rain events leading to inputs of inorganic nutrients such as phosphate, ammonium and nitrate through the watershed with time lags of about 1 week. In contrast, blooms of Alexandrium catenella/tamarense can occur following periods of 3 weeks without precipitation and no significant input of conventional nutrients such as nitrate and phosphate. Field results also indicate a significant drop (from 22–25 to 15–16 μM over 3 days) in dissolved organic nitrogen (DON) at the bloom peak, as well as a significant inverse relationship between A. catenella/tamarense cell density and DON concentrations that is not apparent for diatom blooms. Such dinoflagellate blooms are also associated with elevated (6–9 μM) ammonium...
Tipo: Text
Palavras-chave: Alexandrium catenella/tamarense; Non classical blooms; Organic nitrogen; Ammonium; Growth rate.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00194/30488/29758.pdf

Modelling the accumulation of PSP toxins in Thau Lagoon oysters (Crassostrea gigas) from trials using mixed cultures of Alexandrium catenella and Thalassiosira weissflogii
Provedor de dados: 5
Autores: Lassus, Patrick; Amzil, Zouher; Baron, Regis; Sechet, Veronique; Barille, Laurent; Abadie, Eric; Bardouil, Michele; Sibat, Manoella; Truquet, Philippe; Berard, Jean-baptiste; Gueguen, Marielle.
In October and November 2003 a bloom of the toxic dinoflagellate Alexandrium catenella was observed in the North-east zone of Thau lagoon (French Mediterranean coast). Sea water samples were collected every hours to evaluate time-related variations of phytoplankton concentrations and to compare the relative ratio of A. catenella versus other phytoplankton species during the outbreak. From these observations, trials using recirculated sea water systems were performed to: i) evaluate the physiological effects on oyster of increasing proportions of A. catenella within a mixed microalgal diet where the non-toxic diatom Thalassiosira weissflogii was present at concentration: 1500 cells ml(-1), ii) compare the effect of two temperatures (12 degrees C and 18...
Tipo: Text
Palavras-chave: Mediterranean Sea; Alexandrium catenella; Toxin content; Model; Crassostrea gigas; Paralytic shellfish poisoning.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2624.pdf