Consulta: creatorFacets:"Almeida,AM"
Registros recuperados: 4
Data/hora: 09/06/2024 04:26:30

Duplex Real-Time PCR Using Sybr Green I for Quantification and Differential Diagnosis between Salmonella Enteritidis and Salmonella Typhimurium
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Rubio,MS; Penha Fº,RAC; Almeida,AM; Barbosa,FO; Berchieri Jr,A.
ABSTRACT The incidence of foodborne diseases caused by the genus Salmonella spp. in industrialized countries is often high in epidemiological surveys. Obtaining a rapid diagnostic test for identification of bacteria is crucial in order to rapidly implement control measures to contain bacterial spread, to reduce losses in animal production and to avoid risks from food-borne infections to human health. The aim of this study was to standardize duplex real-time PCR using SYBr Green I for differential and quantitative diagnosis of S. Typhimurium and S. Enteritidis. According to the experiment, the melting temperature of 85°C was observed for a 206bp amplified product when S. Enteritidis DNA was added to the reaction. S. Typhimurium DNA showed that the melting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Identification; Melting curve; Non-typhoidal salmonellosis; Standard curve.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100317

Antimicrobial Resistance in Salmonella Serovars Isolated From an Egg-Producing Region in Brazil
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Benevides,VP; Rubio,MS; Alves,LBR; Barbosa,FO; Souza,AIS; Almeida,AM; Casas,MRT; Guastalli,EAL; Soares,NM; Berchieri Jr,A.
ABSTRACT Fowl paratyphoid infections are caused by different Salmonella serovars that can affect a wide range of hosts. Due to its complex epidemiology, Salmonella serovar identification is crucial for the development and implementation of monitoring and control programs in poultry farms. Moreover, the characterization of the antimicrobial resistance profiles of Salmonella strains isolated from livestock is relevant to public health because they are a common causative agent of foodborne diseases. The objective of this study was to investigate the presence of Salmonella spp. and to identify the antimicrobial resistance profiles of strains isolated in the midwestern region of São Paulo state, which accounts for the highest production of table eggs in Brazil....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial susceptibility; Foodborne disease; Salmonellosis; Multi-drug resistance; Public health.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200319

Evaluation of Disinfectants Used in Pre-Chilling water Tanks of Poultry Processing Plants
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Guastalli,BHL; Batista,DFA; Souza,AIS; Guastalli,EAL; Lopes,PD; Almeida,AM; Prette,N; Barbosa,FO; Stipp,DT; Freitas Neto,OC.
ABSTRACT In poultry processing plants, disinfectants are often added to pre-chilling water tanks to reduce microbial contamination. The present study aimed at evaluating the effect of five disinfectants (acidified sodium chlorite, alkyl dimethyl benzyl ammonium chloride, chlorine dioxide, peracetic acid, and sodium hypochlorite) on the populations of food quality indicator microorganisms and on Salmonella Enteritidis (SE) in the presence and absence of organic matter. The results showed that chlorine dioxide and sodium hypochlorite did not reduce microbial carcass counts. On the other hand, acidified sodium chlorite, alkyl dimethyl benzyl ammonium chloride and peracetic acid reduced total and fecal coliform counts. Peracetic acid reduced the number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler carcasses; Disinfectant; Indicator microorganisms; Pre-chilling tank; Salmonella Enteritidis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200217

Experimental infection of one-day-old chicks with Salmonella Serotypes Previously isolated from poultry facilities, wild birds, and swine
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Sousa,E de; Werther,K; Berchieri Junior,A; Almeida,AM; Ardisson,FA; Silva,AC; Candioto,CG; Fernandes,SA.
In order to maintain the high production and export rates achieved by the Brazilian poultry industry, it is necessary to prevent and control certain disease agents, such as Salmonella spp. Using bacterial cultures, the aim of the present study was to investigate the prevalence of Salmonella spp. in specimens collected from broiler facilities. Local wild birds were also sampled, as well as the feces of swine housed on the poultry farm. After sample collection, the isolated serotypes were subsequently inoculated into broiler chicks to determine their effects. Positive samples were collected from the following locations in the poultry facilities: poultry litter (S. serotype 4,5,12:R:-; S. Heidelberg; S. Infantis), broiler feces (S. Heidelberg; S. serotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriology; Disease of poultry; Diseases of wild birds.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400003