Consulta: creatorFacets:"Almeida,Daniela Volcan"
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Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy
Provedor de dados: 74
Autores: Figueiredo,Márcio de Azevedo; Lanes,Carlos Frederico Ceccon; Almeida,Daniela Volcan; Marins,Luis Fernando.
In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy where a reporter transgene is co-injected with a transgene of interest so that potential germline founders can be easily identified. Transgenic zebrafish (Danio rerio) were produced using two transgenes, both comprised of the carp beta-actin promoter driving the expression of either the green fluorescent protein (GFP) reporter gene or the growth hormone cDNA from the marine silverside fish Odonthestes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transgenesis; Genetically modified fish; Microinjection; Growth hormone cDNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100008