Consulta: creatorFacets:"Alves,J. B."
Registros recuperados: 3
Data/hora: 09/06/2024 13:42:39

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.
ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyperimmunization; Irradiation; Crotalus durissus terrificus; ELISA; Serum neutralization; Detoxification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300008

Laboratory evaluation of young ovines inoculated with natural or 60co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Ferreira Junior,R. S.; Nascimento,N.; Couto,R.; Alves,J. B.; Meira,D. A.; Barraviera,B.
Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; Hyperimmunization; Ovines; Antivenom; Irradiation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400009

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Ferreira Junior,R. S.; Nascimento,N.; Martinez,J. C.; Alves,J. B.; Meira,D. A.; Barraviera,B.
ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venom; Irradiation; Bothrops; ELISA; Serum production; Detoxification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400006