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Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates
Provedor de dados: 58
Autores: Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes.
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004