Consulta: creatorFacets:"Andrade,João Ramos Costa"
Registros recuperados: 2
Data/hora: 12/06/2024 15:36:26

Molecular investigation of tRNA genes integrity and its relation to pathogenicity islands in Shiga toxin-producing Escherichia coli (STEC) strains
Provedor de dados: Genetics and Molecular Biology
Autores: Novais,Rogério Carlos; Chaves,Marcela Cassin; Gonzalez,Alice Gonçalves Martins; Andrade,João Ramos Costa.
tRNA genes are known target sites for the integration of pathogenicity islands (PAI) and other genetic elements, such as bacteriophages, into bacterial genome. In most STEC (Shiga toxin-producing Escherichia coli), the PAI called LEE (locus of enterocyte effacement) is related to bacterial virulence and is mostly associated to the tRNA genes selC and pheU. In this work, we first investigated the relationship of LEE with tRNA genes selC and pheU in 43 STEC strains. We found that 28 strains (65%) had a disrupted selC and/or pheU. Three of these strains (637/1, 650/5 and 654/3) were chosen to be submitted to a RAPD-PCR technique modified by the introduction of specific primers (corresponding to the 5'end of genes selC and pheU) into the reaction, which we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenicity islands; TRNA; STEC; RAPD-PCR; E.coli.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400020

Genetic analysis of Escherichia coli strains carrying enteropathogenic Escherichia coli (EPEC) markers, isolated from children in Rio de Janeiro city, Brazil
Provedor de dados: Brazilian Journal of Microbiology
Autores: Regua-Mangia,Adriana Hamond; Gomes,Tânia Aparecida Tardelli; Andrade,João Ramos Costa; Vieira,Mônica Aparecida Midolli; Gonzalez,Alice Gonçalves Martins; Zahner,Viviane; Irino,Kinue; Teixeira,Lúcia Martins.
In the present study, 47 enteropathogenic Escherichia coli strains identified according to serotyping, presence of eae, bfp and EAF sequences, adherence phenotype and ability to induce attaching-effacing lesions were analyzed by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MLEE), and the presence of LEE genes (eae, espA, espB, tir) as well as the respective alleles. Amplification of LEE genes subtypes revealed 18 different pathotypes. Typing of the eae gene showed that most strains contained nontypable intimin (42%) followed by beta (35%), gamma and alpha genes (12% each). PFGE analysis revealed a variable degree of polymorphism among isolates and, in general, no clear correlation was observed among PFGE profiles and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteropathogenic Escherichia coli; Genetic diversity; Virulence markers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500013