Consulta: creatorFacets:"Andreote,Fernando Dini"
Registros recuperados: 16
Data/hora: 16/06/2024 06:28:27

Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
Provedor de dados: 74
Autores: Rudi Emerson de Lima,Procópio; Araújo,Welington Luiz; Andreote,Fernando Dini; Azevedo,João Lúcio.
A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green fluorescent protein; Bacteria; Host plant; Eucalyptus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100018

Differential expression of the pr1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions and during pathogenesis
Provedor de dados: 74
Autores: Leão,Mariele Porto Carneiro; Tiago,Patricia Vieira; Andreote,Fernando Dini; de Araújo,Welington Luiz; de Oliveira,Neiva Tinti.
The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. In addition, the pr1A gene has been used for the genetic improvement of strains used in pest control. In this study we used quantitative RT-PCR to assess the relative expression levels of the pr1A gene in M. anisopliae and M. acridum during growth in different culture conditions and during infection of the sugar cane borer,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Diatraea saccharalis; Quantitative RT-PCR; Expression pattern.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100086

Analysis of 16S rRNA and mxaF genes reveling insights into Methylobacterium niche-specific plant association
Provedor de dados: 74
Autores: Dourado,Manuella Nóbrega; Andreote,Fernando Dini; Dini-Andreote,Francisco; Conti,Raphael; Araújo,Janete Magali; Araújo,Welington Luiz.
The genus Methylobacterium comprises pink-pigmented facultative methylotrophic (PPFM) bacteria, known to be an important plant-associated bacterial group. Species of this group, described as plant-nodulating, have the dual capacity of producing cytokinin and enzymes, such as pectinase and cellulase, involved in systemic resistance induction and nitrogen fixation under specific plant environmental conditions. The aim hereby was to evaluate the phylogenetic distribution of Methylobacterium spp. isolates from different host plants. Thus, a comparative analysis between sequences from structural (16S rRNA) and functional mxaF (which codifies for a subunit of the enzyme methanol dehydrogenase) ubiquitous genes, was undertaken. Notably, some Methylobacterium spp....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phylogenetic diversity; Methylotrofics; PPFM; Plant-bacteria interaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100020

Resistência a benzimidazóis por Guignardia citricarpa
Provedor de dados: 86
Autores: Rodrigues,Maria Beatriz Calderan; Andreote,Fernando Dini; Spósito,Marcel Bellato; Aguillar-Vildoso,Carlos Ivan; Araújo,Welington Luiz; Pizzirani-Kleiner,Aline Aparecida.
O objetivo deste trabalho foi avaliar a resistência de Guignardia citricarpa aos fungicidas carbendazim e piraclostrobina, por meio de avaliação do crescimento em meio de cultura, decomposição de tecido foliar e produção de corpos de frutificação. Para isso, o fungo G. citricarpa foi isolado de lesões de frutos de laranja (Citrus sinensis), produzidos em área com intensa aplicação de fungicida. Os isolados obtidos foram avaliados quanto à sensibilidade aos fungicidas piraclostrobina e carbendazim, nas dosagens de 0,5, 1 e 2 µg mL-1 de i.a., para se verificar o efeito da pressão de seleção causada pelo uso destes compostos em áreas citrícolas. Embora tenha sido observada redução efetiva no número de estruturas reprodutivas e na decomposição de folhas e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mancha-preta dos citros; Pinta-preta; Tolerância a fungicidas; Citricultura.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300004

Caracterização da comunidade bacteriana endofítica de citros por isolamento, PCR específico e DGGE
Provedor de dados: 86
Autores: Lacava,Paulo Teixeira; Andreote,Fernando Dini; Araújo,Welington Luiz; Azevedo,João Lúcio.
O objetivo deste trabalho foi caracterizar a comunidade bacteriana endofítica de plantas assintomáticas (escapes) e afetadas pela clorose variegada dos citros (CVC) por meio de isolamento em meio de cultura, técnica de gradiente desnaturante em gel de eletroforese (DGGE) e detecção de Methylobacterium mesophilicum e Xyllela fastidiosa por meio de PCR específico, para estudar esta comunidade e sua relação com a ocorrência da CVC. A análise da comunidade bacteriana via DGGE permitiu a detecção de X. fastidiosa, bem como Klebsiella sp. e Acinetobacter sp. como endófitos de citros. Foram observados também Curtobacterium sp., Pseudomonas sp., Enterobacter sp. e Bacillus spp. Utilizando primers específicos, Methylobacterium mesophilicum e X. fastidiosa também...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus sinensis; Xylella fastidiosa; Methylobacterium; Bactérias endofíticas.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000400013

Composição diferencial das comunidades bacterianas na rizosfera de variedas de cana-de-açúcar
Provedor de dados: 90
Autores: Costa,Diogo Paes da; Dias,Armando Cavalcante Franco; Durrer,Ademir; Andrade,Pedro Avelino Maia de; Gumiere,Thiago; Andreote,Fernando Dini.
A cultura da cana-de-açúcar é de extrema importância no cenário agrícola nacional. No entanto, pouco se sabe sobre a estruturação das comunidades microbianas associadas aos solos e às rizosferas de tais plantas. O objetivo deste trabalho foi avaliar a estrutura e diversidade das comunidades de bactérias associadas ao solo e à rizosfera de seis variedades de cana-de-açúcar cultivadas no Estado de São Paulo (Brasil). As análises foram realizadas com base em métodos independentes de cultivo, em que a técnica de PCR-DGGE revelou alterações na rizosfera para os grupos de bactérias totais e também para os grupos de Alphaproteobacteria e Betaproteobacteria. Após essa análise, quatro amostras (três de rizosfera e uma de solo) foram usadas para o sequenciamento da...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Métodos independentes de cultivo; Genes ribossomais; Diversidade bacteriana; Sequenciamento de DNA.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832014000600004

Exploring interactions of plant microbiomes
Provedor de dados: 63
Autores: Andreote,Fernando Dini; Gumiere,Thiago; Durrer,Ademir.
A plethora of microbial cells is present in every gram of soil, and microbes are found extensively in plant and animal tissues. The mechanisms governed by microorganisms in the regulation of physiological processes of their hosts have been extensively studied in the light of recent findings on microbiomes. In plants, the components of these microbiomes may form distinct communities, such as those inhabiting the plant rhizosphere, the endosphere and the phyllosphere. In each of these niches, the "microbial tissue" is established by, and responds to, specific selective pressures. Although there is no clear picture of the overall role of the plant microbiome, there is substantial evidence that these communities are involved in disease control, enhance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial communities; Rhizosphere; Endophytes; Phyllosphere.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000600013

Genetic variability and vegetative compatibility of Erythricium salmonicolor isolates
Provedor de dados: 63
Autores: Sebastianes,Fernanda Luiza de Souza; Maki,Cristina Sayuri; Andreote,Fernando Dini; Araújo,Welington Luiz; Pizzirani-Kleiner,Aline Aparecida.
The Pink Disease is caused by Erythricium salmonicolor, which attacks broad hosts, such as citrus, coffee, rubber, Eucalyptus spp. and Acacia spp., infecting mainly branches. This disease became a serious problem in Brazil, reducing the citrus production up to 10%. However the genetic diversity and compatibility of the fungus E. salmonicolor from Brazilian citrus plants is not yet evaluated. Therefore, the aims of this study were to evaluate: i) the genetic variability of E. salmonicolor in the São Paulo and Minas Gerais States by the RAPD technique, and ii) the vegetative compatibility between these isolates. After RAPD analysis, six distinct groups were observed without correlation between the isolation site or host species. In the vegetative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corticium salmonicolor; RAPD; Pink Disease; Hyphal fusion; Heterokariosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000200009

Deslocamento miscível de um efluente de indústria de explosivo em colunas de solo
Provedor de dados: 53
Autores: Garcia,William Vilar; Alcântara,Marco Aurélio Kondracki de; Camargo,Otávio Antonio de; Izário Filho,Hélcio José; Andreote,Fernando Dini.
Este estudo teve como objetivo avaliar alguns impactos decorrentes do deslocamento miscível de efluente de nitração de uma indústria de explosivos aplicado em colunas de um Latossolo Amarelo, horizonte B (LA-B), submetido aos tratamentos: adição de carbonatos (BASE), ácidos (ACID), fosfato (FOSF), carbonatos e fosfato (BASE-FOSF) e ácidos e fosfatos (ACID-FOSF). A recuperação de nitrogênio em relação ao total aplicado varia entre 10,1 (ACID) e 65,5% (BASE). Há correlação significativa entre as curvas de transposição de N obtidas experimentalmente e as simuladas pelo aplicativo STANMOD para a maioria das colunas (p&lt;0,001). A exceção ocorreu para ACID-FOSF (p=0,202). Não há correlação entre carga eletrostática líquida (CEL) e as variáveis de ajuste do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deslocamento miscível; Nitrogênio; Curvas de transposição; PCR-DGGE.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052012000100015

Endo-and exoglucanase activities in bacteria from mangrove sediment
Provedor de dados: 58
Autores: Soares Júnior,Fábio Lino; Dias,Armando Cavalcante Franco; Fasanella,Cristiane Cipola; Taketani,Rodrigo Gouvêa; Lima,André Oliveira de Souza; Melo,Itamar Soares; Andreote,Fernando Dini.
The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulose endo-1; 4-β-D-glucanase exo-1; 4-β-D-glucanase salinity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048

Screening of endoglucanase-producing bacteria in the saline rhizosphere of Rhizophora mangle
Provedor de dados: 58
Autores: Sá,André Luís Braghini; Dias,Armando Cavalcante Franco; Quecine,Maria Carolina; Cotta,Simone Raposo; Fasanella,Cristiane Cipola; Andreote,Fernando Dini; Melo,Itamar Soares de.
In screening the culturable endoglucanase-producing bacteria in the rhizosphere of Rhizophora mangle, we found a prevalence of genera Bacillus and Paenibacillus. These bacteria revealed different activities in endoglucolysis and biofilm formation when exposed to specific NaCl concentrations, indicating modulated growth under natural variations in mangrove salinity.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacillus; Paenibacillus; Ecological behavior.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100025

Assessing the diversity of bacterial communities associated with plants
Provedor de dados: 58
Autores: Andreote,Fernando Dini; Azevedo,João Lúcio; Araújo,Welington Luiz.
Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant-bacteria interactions; Molecular techniques; Multivariate analysis; Endophytes; Rhizosphere.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300001

Methylobacterium-plant interaction genes regulated by plant exudate and quorum sensing molecules
Provedor de dados: 58
Autores: Dourado,Manuella Nóbrega; Bogas,Andrea Cristina; Pomini,Armando M.; Andreote,Fernando Dini; Quecine,Maria Carolina; Marsaioli,Anita J.; Araújo,Welington Luiz.
Bacteria from the genus Methylobacterium interact symbiotically (endophytically and epiphytically) with different plant species. These interactions can promote plant growth or induce systemic resistance, increasing plant fitness. The plant colonization is guided by molecular communication between bacteria-bacteria and bacteria-plants, where the bacteria recognize specific exuded compounds by other bacteria (e.g. homoserine molecules) and/or by the plant roots (e.g. flavonoids, ethanol and methanol), respectively. In this context, the aim of this study was to evaluate the effect of quorum sensing molecules (N-acyl-homoserine lactones) and plant exudates (including ethanol) in the expression of a series of bacterial genes involved in Methylobacterium-plant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophyte; Methylobacterium; Quantitative PCR (qPCR); Plant-bacteria Interaction; Homoserine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400044

Interspecific variation of the bacterial community structure in the phyllosphere of the three major plant components of mangrove forests
Provedor de dados: 58
Autores: Dias,Armando Cavalcante Franco; Taketani,Rodrigo Gouveia; Andreote,Fernando Dini; Luvizotto,Danice Mazzer; Silva,João Luis da; Nascimento,Rosely dos Santos; Melo,Itamar Soares de.
Mangrove forests encompass a group of trees species that inhabit the intertidal zones, where soil is characterized by the high salinity and low availability of oxygen. The phyllosphere of these trees represent the habitat provided on the aboveground parts of plants, supporting in a global scale, a large and complex microbial community. The structure of phyllosphere communities reflects immigration, survival and growth of microbial colonizers, which is influenced by numerous environmental factors in addition to leaf physical and chemical properties. Here, a combination of culture-base methods with PCR-DGGE was applied to test whether local or plant specific factors shape the bacterial community of the phyllosphere from three plant species (Avicenia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture-independent profiling; Plant genotype; Surface leaves.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200030

Temporal assessment of microbial communities in soils of two contrasting mangroves
Provedor de dados: 58
Autores: Rigonato,Janaina; Kent,Angela D.; Gumiere,Thiago; Branco,Luiz Henrique Zanini; Andreote,Fernando Dini; Fiore,Marli Fátima.
ABSTRACT Variations in microbial communities promoted by alterations in environmental conditions are reflected in similarities/differences both at taxonomic and functional levels. Here we used a natural gradient within mangroves from seashore to upland, to contrast the natural variability in bacteria, cyanobacteria and diazotroph assemblages in a pristine area compared to an oil polluted area along a timespan of three years, based on ARISA (bacteria and cyanobacteria) and nifH T-RFLP (diazotrophs) fingerprinting. The data presented herein indicated that changes in all the communities evaluated were mainly driven by the temporal effect in the contaminated area, while local effects were dominant on the pristine mangrove. A positive correlation of community...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangrove; Cyanobacteria; Diazotrophs; Oil contamination; NifH T-RFLP; ARISA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100087

Genetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques
Provedor de dados: 58
Autores: Dini-Andreote,Francisco; Pietrobon,Vivian Cristina; Andreote,Fernando Dini; Romão,Aline Silva; Spósito,Marcel Bellato; Araújo,Welington Luiz.
The Alternaria brown spot (ABS) is a disease caused in tangerine plants and its hybrids by the fungus Alternaria alternata f. sp. citri which has been found in Brazil since 2001. Due to the recent occurrence in Brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. Here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of A. alternata f. sp. citri from citrus plants and four endophytic isolates from mango (one Alternaria tenuissima and three Alternaria arborescens). The application of two molecular markers Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) had revealed the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternaria brown spot; Molecular markers; Citrus plants; PCA analysis; Genetic variability.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300032