Consulta: creatorFacets:"Araújo Júnior,João Pessoa"
Registros recuperados: 8
Data/hora: 11/06/2024 05:24:13

Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection
Provedor de dados: 80
Autores: Gatto,Mariana; Oliveira,Larissa Ragozo Cardoso; Dias,Fernanda De Nuzzi; Araújo Júnior,João Pessoa; Lima,Carlos Roberto Gonçalves; Lordelo,Eliana Peresi; Santos,Rodrigo Mattos dos; Kurokawa,Cilmery Suemi.
Abstract  Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y.  Methods The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student's paired t-test and independent groups were compared using Student's unpaired t-test.  Results Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas disease; Trypanosoma cruzi; Immune response; Cytokines; Q-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100320

Zika detection: comparison of methodologies
Provedor de dados: 58
Autores: Colombo,Tatiana Elias; Terzian,Ana Carolina Bernardes; Araújo Júnior,João Pessoa; Parreira,Ricardo; Cabrera,Eliana Márcia Sotello; Santos,Izalco Nuremberg Penha dos; Reis,Andréia Francesli Negri; Costa,Fabiana Rodrigues; Cruz,Lilian Elisa Arão Antônio; Rombola,Patrícia Lopes; Nogueira,Maurício Lacerda.
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zika virus; Detection; Sensitivity; Specificity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144

Detection of enterotoxins genes in coagulase-negative staphylococci isolated from foods
Provedor de dados: 58
Autores: Cunha,Maria de Lourdes Ribeiro de Souza da; Peresi,Eliana; Calsolari,Regina Adriana Oliveira; Araújo Júnior,João Pessoa.
Staphylococcal food poisoning is caused by ingestion of enterotoxins preformed in the food contaminated essentially through human manipulation or raw material obtained from animals. Although coagulase-positive Staphylococcus aureus is the main agent responsible for food intoxication, some researches emphasise that coagulase-negative staphylococci (CNS) are able to produce staphylococcal enterotoxins and may be a potential cause of food poisoning. In the present study CNS were isolated from foods and the toxigenic capacity of the strains determined. A total of 88 food samples were analysed and 22.7% were positive for CNS strains. Staphylococcal counts ranged from 3.0 x 10² to 1.4 x 10(6) CFU/g or mL of food examined. S. epidermidis predominated among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; PCR; Enterotoxins.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100013

Colapso induzido pelo exercício em um Labrador Retriever
Provedor de dados: 65
Autores: Basso,Roberta Martins; Oliveira-Filho,José Paes de; Palumbo,Mariana Isa Poci; Zakia,Luiza Stachewski; Araújo Júnior,João Pessoa; Borges,Alexandre Secorun.
O colapso induzido pelo exercício (EIC) é uma enfermidade hereditária caracterizada por fraqueza muscular, dificuldade de locomoção e colapso após atividade física intensa. Esta enfermidade autossômica recessiva afeta principalmente cães jovens da raça Labrador Retriever e decorre da mutação c.767G&gt;T no gene codificador da proteína dinamina 1 (DNM1). O objetivo deste trabalho é relatar o primeiro caso de EIC em Labrador Retriever no Brasil. O teste molecular para detectar a mutação responsável pela EIC confirmou o diagnóstico clínico em um Labrador Retriever com histórico de fraqueza muscular e colapso após exercício. Uma vez diagnosticada no Brasil, ressalta-se a importância de inserir a EIC entre os diagnósticos diferenciais das enfermidades...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Exercício; Mutação; Cães; Sequenciamento; DNM1.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901629

Vírus da leucemia felina: análise da classificação da infecção, das técnicas de diagnóstico e da eficácia da vacinação com o emprego de técnicas sensíveis de detecção viral
Provedor de dados: 65
Autores: Figueiredo,Andreza Soriano; Araújo Júnior,João Pessoa.
O Vírus da leucemia felina (FeLV) pertence à família Retroviridae, gênero Gammaretrovirus. Diferentemente de outras retroviroses, uma parcela dos gatos jovens e adultos exposta ao FeLV não apresenta antigenemia/viremia, de acordo com as técnicas convencionais de detecção viral, como isolamento em cultivo celular, imunofluorescência direta e ELISA. O emprego de técnicas de maior sensibilidade para detecção e quantificação viral, como o PCR quantitativo, permitiu a identificação de animais positivos para a presença de DNA proviral e RNA na ausência de antigenemia/viremia e, com isso, um refinamento da análise das diferentes evoluções da infecção. Assim, reclassificou-se a patogenia do FeLV em 4 categorias: infecção abortiva, regressiva, latente e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antigenemia; Viremia; Patogenia; PCR quantitativo; Vacinação; FeLV.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001100017

Occurrence of feline immunodeficiency virus infection in cats
Provedor de dados: 65
Autores: Lara,Valéria Maria; Taniwaki,Sueli Akemi; Araújo Júnior,João Pessoa.
The occurrence of feline immunodeficiency virus (FIV) in Brazil has been previously described. This study aimed to investigate the frequency of FIV infection in 454 blood samples from healthy and sick domestic cats from 13 cities of São Paulo State, Brazil as well as to evaluate the risk factors associated with the infection. The results showed that 14.7% (67/454) of the cats were infected with FIV. The clinical evaluation showed that 29.2% of the FIV-positive animals were sick, while 7.3% did not show any type of clinical manifestation. In addition, the vast majority (23.1%) of positive cases corresponded to free-roaming owned cats. The incidence of FIV infection was higher in males (20.3%) than in females (9.7%). The results suggest that certain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feline immunodeficiency virus; Epidemiology; Cats; FIV; Risk factors.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000800024

Transcriptomic analysis of differential gene expression reveals an increase in COX2 levels during in vitro canine herpesvirus infection
Provedor de dados: 65
Autores: Kurissio,Jacqueline Kazue; Araújo Júnior,João Pessoa.
ABSTRACT: Canine herpesvirus (CaHV-1) affects canids worldwide, causing death in neonates and immunosuppressed hosts. Acute infection by CaHV-1 can cause reproductive, respiratory, and neurological problems in adult animals. Viral pathogenesis and host genes expressions during of CaHV-1infection are not clearly understood. In the present study, the transcriptome of canine kidney cell Mardin-Darby (MDCK) infected in vitro with canine herpesvirus was explored. For this, RNA sequencing (RNA-seq) of the samples in different moments during infection was carried out. Subsequently, the transcriptomic analysis genes related to cell activities and process involved to viral cycle infection were evaluated until 32h post-inoculation (pi). Among evaluated genes, was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transcriptome; Herpesvirus; CaHV-1; PTGS2; COX2; Canine pathogen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000451

Felis catus gammaherpesvirus 1 (FcaGHV1) and coinfections with feline viral pathogens in domestic cats in Brazil
Provedor de dados: 65
Autores: Kurissio,Jacqueline Kazue; Rodrigues,Marianna Vaz; Taniwaki,Sueli Akemi; Zanutto,Marcelo de Souza; Filoni,Claudia; Galdino,Maicon Vinícius; Araújo Júnior,João Pessoa.
ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1) may causes an asymptomatic infection that result in an efficient transmission and subsequently dissemination of the virus in feline population. This study used molecular detection by qPCR (quantitative PCR) based on DNA polymerase gene fragment amplification to evaluate the occurrence of FcaGHV1 and its correlation with other feline viral pathogens, such as Carnivore protoparvovirus 1 (CPPV-1), Felid alphaherpesvirus 1 (FeHV-1), and feline retroviruses such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Of the 182 blood samples evaluated 23.6% (43/182) were positives for FcaGHV1. Approximately 37.9% (33/87) of the samples that tested positive for retrovirus were also were positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gammaherpesvirus; Herpesvirus; Feline pathogen; Infectious diseases; Diagnosis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300451