Consulta: creatorFacets:"Artigaud, Sebastien"
Registros recuperados: 13
Data/hora: 29/05/2024 08:33:27

Deep sequencing of the mantle transcriptome of the great scallop Pecten maximus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Artigaud, Sebastien; Thorne, Michael A. S.; Richard, Joelle; Lavaud, Romain; Jean, Fred; Flye-sainte-marie, Jonathan; Peck, Lloyd S.; Pichereau, Vianney; Clark, Melody S.
RNA-Seq transcriptome data were generated from mantle tissue of the great scallop, Pecten maximus. The consensus data were produced from a time course series of animals subjected to a 56-day thermal challenge at 3 different temperatures. A total of 26,064 contigs were assembled de novo, providing a useful resource for both the aquaculture community and researchers with an interest in mollusc shell production.
Tipo: Text
Palavras-chave: Pecten maximus; RNAseq; Temperature.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00202/31362/82662.pdf

New insights into the seasonal feeding ecology of Pecten maximus using pigments, fatty acids and sterols analyses
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Lavaud, Romain; Artigaud, Sebastien; Le Grand, Fabienne; Donval, Anne; Soudant, Philippe; Flye-sainte-marie, Jonathan; Strohmeier, Tore; Strand, Oivind; Leynaert, Aude; Beker, Beatriz; Chatterjee, Arnab; Jean, Fred.
We combined the use of pigments, fatty acids and sterols as biomarkers of the seasonal variation in food sources of the great scallop Pecten maximus. From March to October 2011, on a biweekly to twice-weekly basis, scallops and seawater from the water column and the water-sediment interface were collected in the Bay of Brest (Brittany, France). Pigment compositions in the seawater and in the stomach and rectum content of the scallops were analyzed by HPLC. Fatty acids and sterols from digestive gland (DG) tissue were analyzed by gas chromatography. Potential relationships between the temporal proportion of each marker in the environment and in the digestive tract were tested using multivariate analysis. Proportions of diatoms and Dinophyceae biomarkers...
Tipo: Text
Palavras-chave: Trophic ecology; Food sources; Pigments; Fatty acids; Trophic marker; Phytoplankton; Pecten maximus.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00431/54244/55772.pdf

Significance of metallothioneins in differential cadmium accumulation kinetics between two marine fish species
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Croizier, Gael; Lacroix, Camille; Artigaud, Sebastien; Le Floch, Stephane; Raffray, Jean; Penicaud, Virginie; Coquille, Valerie; Autier, Julien; Rouget, Marie-laure; Le Bayon, Nicolas; Lae, Raymond; De Morais, Luis Tito.
Impacted marine environments lead to metal accumulation in edible marine fish, ultimately impairing human health. Nevertheless, metal accumulation is highly variable among marine fish species. In addition to ecological features, differences in bioaccumulation can be attributed to species-related physiological processes, which were investigated in two marine fish present in the Canary Current Large Marine Ecosystem (CCLME), where natural and anthropogenic metal exposure occurs. The European sea bass Dicentrarchus labrax and Senegalese sole Solea senegalensis were exposed for two months to two environmentally realistic dietary cadmium (Cd) doses before a depuration period. Organotropism (i.e., Cd repartition between organs) was studied in two storage...
Tipo: Text
Palavras-chave: Metal; Trace element; Dicentrarchus labrax; Solea senegalensis; Chronic dietary exposure.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00437/54810/56480.pdf

In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Foulon, Valentin; Boudry, Pierre; Artigaud, Sebastien; Guérard, Fabienne; Hellio, Claire.
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the...
Tipo: Text
Palavras-chave: Crassostrea gigas; Pacific oyster; Pediveliger larvae; Bioadhesive; Transcriptome.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00475/58677/61172.pdf

Dataset of differentially accumulated proteins in Mucor strains representative of four species grown on synthetic potato dextrose agar medium and a cheese mimicking medium
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Morin-sardin, Stephanie; Jany, Jean-luc; Artigaud, Sebastien; Pichereau, Vianney; Bernay, Benoit; Coton, Emmanuel; Madec, Stephanie.
The data presented are associated with the "Proteomic analysis of the adaptative response of Mucor spp. to cheese environment" (Morin-Sardin et al., 2016) article [11. Mucor metabolism is poorly documented in the literature and while morphology and growth behavior suggest potential adaptation to cheese for some strains, no adaptation markers to cheese environment have been identified for this genus. To establish the possible existence of metabolic functions related to cheese adaptation, we used a gel based 2-DE proteomic approach coupled to LC-MS/MS to analyze three strains from species known or proposed to have a positive or negative role in cheese production as well as a strain from a non-related cheese species. (C) 2017 The Authors. Published by...
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00602/71412/69873.pdf

Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Harney, Ewan; Artigaud, Sebastien; Le Souchu, Pierrick; Miner, Philippe; Corporeau, Charlotte; Essid, Hafida; Pichereau, Vianney; Nunes, Flavia.
Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40 h under either control (pH 8.1, 20 °C), acidified (pH 7.9, 20 °C), warm (pH 8.1, 22 °C) or warm acidified (pH 7.9, 20 °C) conditions. Larvae in acidified conditions were significantly smaller than in the control, but warm acidified conditions mitigated negative effects on size, and increased calcification. A proteomic approach employing two-dimensional electrophoresis (2-DE) was used to quantify proteins and relate their abundance to phenotypic traits. In...
Tipo: Text
Palavras-chave: Crassostrea gigas; Ocean acidification; Larval development; ATP synthase; GAPDH; Superoxide dismutase.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00299/40985/40076.pdf

A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Smits, M.; Artigaud, Sebastien; Bernay, B.; Pichereau, Vianney; Bargelloni, L.; Paillard, Christine.
Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by...
Tipo: Text
Palavras-chave: Bivalve; Diseases; Resistance; Manila clam; Vibrio tapetis; Proteomics; Immune response.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00615/72670/71758.pdf

The oyster Crassostrea gigas, a new model against cancer
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Corporeau, Charlotte; Huvet, Arnaud; Pichereau, Vianney; Delisle, Lizenn; Quéré, Claudie; Dubreuil, Christine; Artigaud, Sebastien; Brenner, Catherine; Meyenberg Cunha-de Padua, Monique; Mazure, Nathalie.
The Warburg effect is one of the hallmarks of cancer cells in humans. It is a true metabolic reprogramming to aerobic glycolysis, allowing cancer cells to meet their particular energy needs for growth, proliferation, and resistance to apoptosis, depending on the microenvironment they encounter within the tumor. We have recently discovered that the Crassostrea gigas oyster can naturally reprogram its metabolism to the Warburg effect. Thus, the oyster becomes a new invertebrate model useful for cancer research. Due to its lifestyle, the oyster C. gigas has special abilities to adapt its metabolism to the extreme changes in the environment in which it is located. The oyster C. gigas is therefore a model of interest to study how the environment can control the...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00499/61064/64516.pdf

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Prieto, Daniel; Markaide, Pablo; Urrutxurtu, Iñaki; Navarro, Enrique; Artigaud, Sebastien; Fleury, Elodie; Ibarrola, Irrintzi; Urrutia, Miren Bego.
The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups...
Tipo: Text
Palavras-chave: Fast-growing; Mussel; Gill; Transcriptome.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00503/61490/65293.pdf

Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Borcier, Elodie; Artigaud, Sebastien; Gaillard, Jean-charles; Armengaud, Jean; Charrier, Gregory; Couteau, Jérôme; Receveur, Justine; Ouddane, Baghdad; Diop, Mamadou; Amara, Rachid; Laroche, Jean; Pichereau, Vianney.
Estuaries are important areas highly vulnerable to anthropogenic pollutions. Therefore, the assessment of estuarine water quality is a major ecological issue. In this study, we sampled juveniles of the European flounder in the “pristine” Canche estuary, and caged them in Canche and in two polluted sites of the Seine estuary, Rouen and Fosse Nord. After one month, the metal and organic pollutants in these sites were assessed, and we evaluated several phenotypic indicators (condition index, RNA/DNA ratios and genotoxicity), and extracted the proteins in fish livers for analysis using a shotgun proteomics approach. The results showed strong modifications in the fish caged in both sites of the Seine estuary, as compared to those caged in Canche. In particular,...
Tipo: Text
Palavras-chave: Estuary water quality; Ecotoxicology; Caging; Suess; Fish physiology; Shotgun proteomics.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00509/62067/66247.pdf

Metal subcellular partitioning determines excretion pathways and sensitivity to cadmium toxicity in two marine fish species.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Le Croizier, Gael; Lacroix, Camille; Artigaud, Sebastien; Le Floch, Stephane; Munaron, Jean-marie; Raffray, Jean; Penicaud, Virginie; Rouget, Marie-laure; Lae, Raymond; Tito De Morais, Luis.
Subcellular cadmium (Cd) partitioning was investigated in the liver of two marine fish species, the European sea bass Dicentrarchus labrax and the Senegalese sole Solea senegalensis, dietary exposed to an environmentally realistic Cd dose for two months followed by a two-month depuration. Cd exposure did not modify Cd cellular partitioning for either species, refuting the spillover hypothesis. Both species contained most of the Cd in the detoxifying fraction but displayed different handling strategies. Cd was largely bound to heat stable proteins (HSP) including metallothioneins (MT) in sea bass while Cd was more linked to metal rich granules (MRG) in sole. Whole liver concentrations and subcellular partitioning were also determined for essential elements....
Tipo: Text
Palavras-chave: Sub-cellular fractionation; Dicentrarchus labrax; Solea senegalensis; Essential element distribution; Elimination; Depuration.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00464/57606/59792.pdf

Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Foulon, Valentin; Artigaud, Sebastien; Buscaglia, Manon; Bernay, Benoit; Fabioux, Caroline; Petton, Bruno; Elies, Philippe; Boukerma, Kada; Hellio, Claire; Guerard, Fabienne; Boudry, Pierre.
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00462/57379/59452.pdf

Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Artigaud, Sebastien; Richard, Joelle; Thorne, Michael A. S.; Lavaud, Romain; Flye-sainte-marie, Jonathan; Jean, Fred; Peck, Lloyd S.; Clark, Melody S.; Pichereau, Vianney.
Background: The capacity of marine species to survive chronic heat stress underpins their ability to survive warming oceans as a result of climate change. In this study RNA-Seq and 2-DE proteomics were employed to decipher the molecular response of the sub-tidal bivalve Pecten maximus, to elevated temperatures. Results: Individuals were maintained at three different temperatures (15, 21 and 25 degrees C) for 56 days, representing control conditions, maximum environmental temperature and extreme warming, with individuals sampled at seven time points. The scallops thrived at 21 degrees C, but suffered a reduction in condition at 25 degrees C. RNA-Seq analyses produced 26,064 assembled contigs, of which 531 were differentially expressed, with putative...
Tipo: Text
Palavras-chave: Marine biology; Metabolism; DNA repair; Transcription regulation; Apoptosis; Energy reserves.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00301/41173/40360.pdf