Consulta: creatorFacets:"Auffret, Mathieu"
Registros recuperados: 4
Data/hora: 08/06/2024 02:08:18

Fine cytochemical localization of lysosomal organelles in phagocytic cells of bivalve molluscs.
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Auffret, Mathieu; Diouris, M; Herry, A.
Bivalve molluscs possess several types of hemolymph cells (hemocytes), involved in internal defense and other physiological functions. Studies in pathological and experimental conditions have shown their micro- and macrophagic ability. However, the mechanisms of intracellular digestion are incompletely known in these organisms. The lysosomal apparatus of hemocytes was therefore investigated in several species by hydrolase localization with the electron microscope. The cytoplasmic granules of granulocytes displayed a strong acid beta -glycerophosphatase activity. Previous observations had shown that these granules may contain phagocytized material. This indicates that intracellular digestion is achieved in these organelles. Acid beta -glycerophosphatase...
Tipo: Text
Palavras-chave: Bivalvia; Phagocytosis; Cells; Ultrastructure; Haemolymph.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1631.pdf

Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Gagnaire, Beatrice; Duchemin, M; Auffret, Mathieu; Thomas-guyon, Helene; Renault, Tristan.
Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Phagocytosis; Hemocytes; Flow cytometry.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3898.pdf

Etudes pathologiques et epidemiologiques des lesions parasitaires chez Ostrea edulis : Premiers resultats d'un recherche prospective comparative chez les principales especes de mollusques des zones ostreicoles de Bretagne nord
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Poder, M; Auffret, Mathieu; Balouet, G.
This paper is a contribution to the study of the Bonamia ostreae epizooty in french european oyster Ostrea edulis : parasite process (hemocytic parasitosis); experimental contaminations on parks and in tanks. First results of an epidemiological watch conducted in oyster culture sites show in molluks mytilidae, pectinidae, cardiidae, veneridae: the absence of Bonamia ostreae forms; the existence of different types of parasitism or commensalisus of inequal pathological importance and especially a parasite of the Marteilia genus in Tapes sp . digestive epithelium. Additionally, a new example of hemocytosercoma is observed in Cerastoderma edule .
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Tapes rhomboides; Tapes pullastra; Mytilus edulis; Cerastoderma edule; Ostrea edulis; Bivalvia; Marteilia; Bonamia ostreae; Comparative studies; Parasitic diseases; Commensalism; Parasitology; Marine mollusks; Epidemiology; Pathology; Pathologie; Mollusques; Bonamia ostreae; Epizootie; Ostrea edulis.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1182.pdf

Fixation d''un anticorps anti-lysozyme dans des cellules de mollusques bivalves
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Auffret, Mathieu; Diouris, M; Herry, A.
A polyclonal rabbit anti-human lysozyme (Dako) was applied on histological sections from bivalve mollusc and revealed with a mouse anti-rabbit IgG fluorescein conjugated antibody to localise cellular lysozyme in tissues where its activity had previously detected by enzymatic techniques. Labelling occurred in the cytoplasm of epithelial cells covering the body. This is in agreement with the intracellular distribution of this enzyme. In the Lucinid Lucinella divaricata gills, the cytoplasm of bacteriocytes was also labelled. This suggests that antibacterial activity occurred in these cells. Control sections indicate that fluorescence was a consequence of true primary antibody fixation. However, its specificity should be verified by electrophoretical...
Tipo: Text
Palavras-chave: Bivalvia; Immunology; Antibodies; Electrophoresis; Histology.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1723.pdf