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COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES
Provedor de dados: Revista Brasileira de Fruticultura
Autores: LUZ,ANNY CAROLYNE DA; PRETTI,IRANY RODRIGUES; BATITUCCI,MARIA DO CARMO PIMENTEL.
ABSTRACT  Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to analyse three RNA extraction methods, Concert™ Plant RNA Reagent (Invitrogen, Carlsbad, CA, USA), TRIzol® Reagent (Invitrogen) and TRIzol® Reagent (Invitrogen)/ice -commercial products specifically designed to extract RNA, and to determine which method is the most effective for extracting RNA from the leaves of passion fruit plants. In contrast to the RNA extracted using the other 2 methods, the RNA extracted using TRIzol® Reagent (Invitrogen) did not have acceptable A260/A280 and A260/A230...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis; RNA extraction; Concert™; TRIzol®; Low temperature.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000100226