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Data/hora: 10/06/2024 14:54:39

Structure analysis of the endoxylanase A gene from Penicillium purpurogenum
Provedor de dados: Biological Research
Autores: CHÁVEZ,RENATO; ALMARZA,CAROLINA; SCHACHTER,KAREN; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME.
Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protein was determined and this allowed the design of oligonucleotides for use in polymerase chain reactions. Different polymerase chain reaction strategies were used to amplify and sequence the entire cDNA and the gene. The gene has an open reading frame of 1450 base pairs, including 8 introns with an average length of 56 base pairs each. Only one copy of this gene is present in the P. purpurogenum genome as shown by Southern blot. The gene encodes a protein of 329...
Tipo: Journal article
Palavras-chave: Family 10 endoxylanases; Gene structure; Penicillium purpurogenum.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009

The acetyl xylan esterase II gene from Penicillium purpurogenum is differentially expressed in several carbon sources, and tightly regulated by pH
Provedor de dados: Biological Research
Autores: CHÁVEZ,RENATO; SCHACHTER,KAREN; NAVARRO,CLAUDIO; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME.
The expression of the acetyl xylan esterase II (axeII) gene from Penicillium purpurogenum is repressed by glucose and induced by xylan, as well as to a small degree by xylose and xylitol. This gene is expressed at neutral pH, but not under alkaline or acidic conditions, in agreement with previous findings for other xylanolytic genes of this organism. This is the first report showing pH regulation of an axe gene.
Tipo: Journal article
Palavras-chave: Acetyl xylan esterase; Gene expression; Penicillium purpurogenum.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100011