Consulta: creatorFacets:"Bahia,M.O."
Registros recuperados: 4
Data/hora: 04/06/2024 10:26:08

Cytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Silva-Pereira,L.C.; Cardoso,P.C.S.; Leite,D.S.; Bahia,M.O.; Bastos,W.R.; Smith,M.A.C.; Burbano,R.R.
Mercury is a xenobiotic metal that is a highly deleterious environmental pollutant. The biotransformation of mercury chloride (HgCl2) into methylmercury chloride (CH3HgCl) in aquatic environments is well-known and humans are exposed by consumption of contaminated fish, shellfish and algae. The objective of the present study was to determine the changes induced in vitro by two mercury compounds (HgCl2 and CH3HgCl) in cultured human lymphocytes. Short-term human leukocyte cultures from 10 healthy donors (5 females and 5 males) were set-up by adding drops of whole blood in complete medium. Cultures were separately and simultaneously treated with low doses (0.1 to 1000 µg/l) of HgCl2 and CH3HgCl and incubated at 37ºC for 48 h. Genotoxicity was assessed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylmercury; Cytotoxicity; Genotoxicity; Mitotic index; Human lymphocytes; Chromosome aberrations.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600012

Investigation of single-strand conformational polymorphism of the TP53 gene in women with a family history of breast cancer
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Burbano,R.R.; Medeiros,A.C.; Mello,A.A.; Lemos,J.A.; Bahia,M.O.; Casartelli,C.
Breast cancer in families with germ line mutations in the TP53 gene has been described in the medical literature. Mutation screening for susceptibility genes should allow effective prophylactic and preventive measures. Using single-strand conformational polymorphism, we screened for mutations in exons 5, 6, 7 and 8 of gene TP53 in the peripheral blood of 8 young non-affected members (17 to 36 years old) of families with a history of breast cancer. Studies of this type on young patients (mean age, 25 years) are very rare in the literature. The identification of these mutations would contribute to genetic counseling of members of families with predisposition to breast cancer. The results obtained did not show any polymorphism indicating mutation. In our...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TP53; Family history of breast cancer; Mutation screening; Early breast cancer.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100010

Cytogenetic description of breast fibroadenomas: alterations related solely to proliferation?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Burbano,R.R.; Lima,E.M.; Khayat,A.S.; Barbieri Neto,J.; Cabral,I.R.; Bastos Jr.,L.; Bahia,M.O.; Casartelli,C.
Twelve breast fibroadenomas were analyzed cytogenetically and only four were found to have clonal alterations. The presence of chromosomal alterations in fibroadenomas must be the consequence of the proliferating process and must not be related to the etiology of this type of lesion. In contrast, the few fibroadenomas that exhibit chromosomal alterations are likely to be those presenting a risk of neoplastic transformation. Clonal numerical alterations involved chromosomes 8, 18, 19, and 21. Of the chromosomal alterations found in the present study, only monosomy of chromosomes 19 and 21 has been reported in breast fibroadenomas. The loss of chromosome 21 was the most frequent alteration found in our sample. The study of benign proliferations and their...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fibroadenomas; Chromosome alterations; Breast cancer.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800005

Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ribeiro,H.F.; Alcântara,D.F.A.; Matos,L.A.; Sousa,J.M.C.; Leal,M.F.; Smith,M.A.C.; Burbano,R.R.; Bahia,M.O.
Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Carcinogenesis; Chromosomal abnormalities; Gastric cancer; Oncogenes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800004