Consulta: creatorFacets:"Barbisan,Luis Fernando"
Registros recuperados: 3
Data/hora: 10/06/2024 13:41:30

Sewage sludge does not induce genotoxicity and carcinogenesis
Provedor de dados: Genetics and Molecular Biology
Autores: Silva,Paula Regina Pereira; Barbisan,Luis Fernando; Dagli,Maria Lúcia Zaidan; Saldiva,Paulo Hilário Nascimento.
Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Sewage genotoxicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400017

Evaluation of cell proliferation and apoptosis in placentas of rats with severe diabetes
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Rudge,Marilza Vieira Cunha; Costa,Elaine; Barbisan,Luis Fernando; Damasceno,Débora Cristina; Bueno,Aline; Saito,Felipe Hiroshi; Calderon,Iracema Mattos Paranhos; Rodrigues,Marcela Marcondes Pinto.
The aim of this work was to analyze the cell proliferation and apoptosis indexes on the 18th and 21st days of pregnancy of diabetic rats and to correlate with maternal glycemia and perinatal outcomes. Placentas from 20 Wistar rats were collected and divided into four experimental groups: control and diabetic of 18 and 21 days of pregnancy. The cell proliferation was analyzed using the PCNA expression and apoptosis by the TUNEL method. It was observed that PCNA and TUNEL indexes decreased from day 18 to 21 of pregnancy in the placentas of diabetic rats and these values were lower than control groups. Diabetic dams presented higher percentage of small for pregnancy age (SPA) fetuses. However, there was no difference between the PCNA and TUNEL indexes in SPA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Cell proliferation; Apoptosis; Placenta; Rat; IUGR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200009

Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis
Provedor de dados: Ciência e Agrotecnologia
Autores: Venâncio,Vinícius Paula; Ferreira,Eric Batista; Brigagão,Maísa Ribeiro Pereira Lima; Paula,Fernanda Borges de Araújo; Barbisan,Luis Fernando; Azevedo,Luciana.
A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aberrant crypt foci; Brazilian savannah; Dimethylhydrazine; Immunohistochemistry; Oxidative stress.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000400008