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Dipyridamole increases the cytotoxicity of cisplatin in human larynx cancer cells in vitro
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rodrigues,M.; Barbosa Junior,F.; Perussi,J.R.
This paper describes the effect of dipyridamole (DIP) on the cytotoxicity of cisplatin in HEp-2 human larynx cancer cells in vitro and the nature of the interaction between cisplatin and dipyridamole. Cytotoxic assays were performed to obtain the IC50 for cisplatin. The cells were treated with 0, 20, 40, 80, 120 or 200 µM cisplatin, with or without a single concentration of DIP and incubated for 60 min at 37ºC and 5% CO2 for 3 days and then counted with a hemocytometer. The accumulation of cisplatin in the cells was measured by atomic absorption and fluorescence was used to determine the membrane binding constant of DIP. In the presence of 10, 20 and 30 µM DIP, the IC50 of cisplatin was reduced by 25, 60 and 82% in HEp-2 cells. Combination index analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cisplatin; Dipyridamole; Synergism; Tumor cells; Cytotoxicity; IC50.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400017