Consulta: creatorFacets:"Beçak,W."
Registros recuperados: 2
Data/hora: 04/06/2024 01:02:20

Production of L1 protein from different types of HPV in Pichia pastoris using an integrative vector
Provedor de dados: 56
Autores: Coimbra,E.C.; Gomes,F.B.; Campos,J.F.; D’arc,M.; Carvalho,J.C.; Mariz,F.C.; Jesus,A.L.S.; Stocco,R.C.; Beçak,W.; Freitas,A.C.
Human papillomavirus (HPV) infection is the most common sexually transmitted disease in the world and is related to the etiology of cervical cancer. The most common high-risk HPV types are 16 and 18; however, the second most prevalent type in the Midwestern region of Brazil is HPV-33. New vaccine strategies against HPV have shown that virus-like particles (VLP) of the major capsid protein (L1) induce efficient production of antibodies, which confer protection against the same viral type. The methylotrophic yeast Pichia pastoris is an efficient and inexpensive expression system for the production of high levels of heterologous proteins stably using a wild-type gene in combination with an integrative vector. It was recently demonstrated that P. pastoris can...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human papillomavirus; Cervical cancer; Pichia pastoris; L1.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200003

Induction of apoptosis in cancer cells by tumor necrosis factor and butyrolactone, an inhibitor of cyclin-dependent kinases
Provedor de dados: 56
Autores: Belizário,J.E.; Sherwood,S.; Beçak,W.
Induction of apoptosis by tumor necrosis factor (TNF) is modulated by changes in the expression and activity of several cell cycle regulatory proteins. We examined the effects of TNF (1-100 ng/ml) and butyrolactone I (100 µM), a specific inhibitor of cyclin-dependent kinases (CDK) with high selectivity for CDK-1 and CDK-2, on three different cancer cell lines: WEHI, L929 and HeLa S3. Both compounds blocked cell growth, but only TNF induced the common events of apoptosis, i.e., chromatin condensation and ladder pattern of DNA fragmentation in these cell lines. The TNF-induced apoptosis events were increased in the presence of butyrolactone. In vitro phosphorylation assays for exogenous histone H1 and endogenous retinoblastoma protein (pRb) in the total cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell cycle; Cyclin-dependent kinases; Cyclin-dependent kinase chemical inhibitors; Tumor necrosis factor.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400016