Consulta: creatorFacets:"Beck,Erwin"
Registros recuperados: 1
Data/hora: 10/06/2024 08:32:10

Transformation of an Argentine spring wheat genotype: optimization of the protocols for particle bombardment of excised immature embryos and rapid isolation of transgenic plants
Provedor de dados: 46
Autores: Souza Canada,Eduardo Daniel; Fettig,Sebastian; Ziegler,Paul; Beck,Erwin.
Klein Brujo was found to be a promising Argentine spring wheat genotype for transformation studies. In the present work we optimized the biolistic transformation of embryogenic scutellar calli from this genotype. We first identified scutellar callus induction media (SCIM) most promising for in vitro embryogenic plant regeneration with Klein Brujo. We then co-bombarded embryos of Klein Brujo and, for comparison, of Bobwhite, a highly transformable wheat line, on these media with 1 &amp;#956;m gold particles coated with two plasmids. One of these contained the marker gene gfp (linked to the CaMV35S-promoter) and the selection gene bar (resistance to phosphinothricin: PPT, linked to the maize Ubi1-promoter), whereas the other contained ipt (encoding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Particle bombardment parameters; Co-transformation; Green fluorescent protein; Phosphinothricin resistance; Transgene segregation.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000100003