Consulta: creatorFacets:"Beirão,P.S.L."
Registros recuperados: 3
Data/hora: 08/06/2024 14:35:55

CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mudado,M.A.; Rodrigues,A.L.; Prado,V.F.; Beirão,P.S.L.; Cruz,J.S.
T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium channel; T-type; Electrophysiology; RT-PCR; GH3 cells.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020

Is there a role for voltage-gated Na+ channels in the aggressiveness of breast cancer?
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Rhana,P.; Trivelato Junior,R.R.; Beirão,P.S.L.; Cruz,J.S.; Rodrigues,A.L.P.
Breast cancer is the most common cancer among women and its metastatic potential is responsible for numerous deaths. Thus, the need to find new targets for improving treatment, and even finding the cure, becomes increasingly greater. Ion channels are known to participate in several physiological functions, such as muscle contraction, cell volume regulation, immune response and cell proliferation. In breast cancer, different types of ion channels have been associated with tumorigenesis. Recently, voltage-gated Na+ channels (VGSC) have been implicated in the processes that lead to increased tumor aggressiveness. To explain this relationship, different theories, associated with pH changes, gene expression and intracellular Ca2+, have been proposed in an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Ion channels; Metastasis; Nav1.5 channel.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700301

Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Mafra,R.A.; Leão,R.M.; Beirão,P.S.L.; Cruz,J.S.
A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebellar granule neurons; Excitatory amino-acid transporter type 2; Patch-clamp; Glutamate; Whole-cell voltage-clamp.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018