Consulta: creatorFacets:"Benevides,Norma Maria Barros"
Registros recuperados: 7
Data/hora: 01/06/2024 18:47:37

Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides
Provedor de dados: 52
Autores: Rodrigues,José Ariévilo Gurgel; Vanderlei,Edfranck de Sousa Oliveira; Bessa,Érika Façanha; Magalhães,Francisco de Araújo; Paula,Regina Célia Monteiro de; Lima,Vilma; Benevides,Norma Maria Barros.
The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine algae; Sulfated macromolecules; Chemical analysis; APTT test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007

Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta) on gastrointestinal smooth muscle contractility
Provedor de dados: 52
Autores: Graça,José Ronaldo Vasconcelos; Bezerra,Mirna Marques; Lima,Vilma; Rodrigues,José Ariévilo Gurgel; Monteiro,Diego Levi Silveira; Quinderé,Ana Luíza Gomes; Amorim,Rodrigo César das Neves; Paula,Regina Célia Monteiro de; Benevides,Norma Maria Barros.
The aim of this work was to study the effect of Halymenia floresia (Hf) on duodenum contractility, and on experimental protocols of gastric compliance (GC) in rats. Fraction Hf2s exhibited a concentration-dependent myocontractile effect (EC50 12.48 µg/ml), and an inhibitory effect after consecutive washing. The contractile response promoted by Hf2s in the duodenum strips was completely inhibited by verapamil, and the effects were prevented in the presence of Ca2+-free medium. The pretreatment with atropine prevented the Hf2s myocontractile effect. Hf2s was also capable to decrease the GC (from 3.8±0.06 to 3.4±0.13 ml, P&lt;0.05), which did not return to basal levels after more 50 min of observation. These results indicated that the algal polysaccharide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Halymenia floresia; Sulfated polysaccharides; Gut motility.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500008

Antimicrobial effect of a crude sulfated polysaccharide from the red seaweed Gracilaria ornata
Provedor de dados: 52
Autores: Amorim,Rodrigo das Neves dos Santos; Rodrigues,José Ariévilo Gurgel; Holanda,Márjory Lima; Quinderé,Ana Luíza Gomes; Paula,Regina Célia Monteiro de; Melo,Vânia Maria Maciel; Benevides,Norma Maria Barros.
The aim of this study was to determine the yield, chemical composition, specific rotation (SR), infrared (IR) spectroscopy and the effect on bacterial growth of a crude sulfated polysaccharide (SP) from the red marine alga G. ornata (Go). Go-1 (25°C), Go-2 (80°C), and Go-3 (80°C) were sequentially extracted and yielded 9.2%. The contents of sulfate (5.88-10.3%) and proteins (0.1-3.7%) were small. The values of SR were [µ]D20°f -19.0, -51.0, and -56.5, respectively. IR spectrums showed the presence of galactose-4 sulfate and absence of 3,6-anydrogalactose-2 sulfate, galactose-6 sulfate and galactose-2 sulfate. SR and IR techniques confirmed SPs. Go-3 was tested on the growth of bacteria (Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogens,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhodophyta; Sulfated macromolecules; Chemical analysis; Antimicrobial.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200001

Isolamento, fracionamento e atividade anticoagulante de iota-carragenanas da Solieria filiformis
Provedor de dados: 65
Autores: Rodrigues,José Ariévilo Gurgel; Araújo,Ianna Wivianne Fernandes de; Paula,Gabriela Almeida de; Bessa,Érika Façanha; Lima,Ticiana de Brito; Benevides,Norma Maria Barros.
Este estudo teve como objetivo isolar, fracionar e avaliar o potencial anticoagulante de iota-carragenanas (i-CARs) da rodofícea Solieria filiformis, quando obtidas por dois métodos de extração (M I e M II). As i-CARs foram isoladas com papaína bruta em tampão acetato de sódio 0,1M (pH 5,0), contendo cisteína 5mM e EDTA 5mM (M I) ou água (80°C) (M II) e, em seguida, determinada sua composição química de carboidratos totais, sulfato livre (SL) e proteínas contaminantes. As i-CARs foram submetidas à cromatografia de troca iônica (DEAE-celulose) usando um gradiente de cloreto de sódio, sendo avaliado o tempo de tromboplastina parcial ativada (TTPA) e tempo de protrombina das frações obtidas e comparadas à heparina (193UI mg-1). Uma fração anticoagulante...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rodofícea; Polissacarídeos sulfatados; TTPA; Métodos de extração.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100010

Pesquisadores avaliam características químicas da alga marinha vermelha Hypnea musciformis
Provedor de dados: 65
Autores: Benevides,Norma Maria Barros.
Tipo: Info:eu-repo/semantics/other
Ano: 2024
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011010700002

An antithrombin-dependent sulfated polysaccharide isolated from the green alga Caulerpa cupressoides has in vivo anti- and prothrombotic effects
Provedor de dados: 65
Autores: Rodrigues,José Ariévilo Gurgel; Queiroz,Ismael Nilo Lino de; Quinderé,Ana Luíza Gomes; Vairo,Bruno Cunha; Mourão,Paulo Antônio de Souza; Benevides,Norma Maria Barros.
Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of antithrombotic activity regarding green algae SPs has been reported. Caulerpa cupressoides (Chlorophyta) has three different SPs fractions (SP1, SP2 and SP3). We investigated the effects of SP2 on thrombin activity by antithrombin and in an experimental model of venous thrombosis in rats. The inhibition of thrombin assay was evaluated using antithrombin (AT) in the presence of SP2 and the antithrombotic activity was investigated in rats with thromboplastin as the thrombogenic stimulus. The anticoagulant effects of SP2 are suggested be due to the potentiation of thrombin inhibition by antithrombin (IC50 ~ 10.0µg mL-1) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Green alga; Sulfated polymers; Biological events; Coagulation system; Thrombosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400014

Isolamento, fracionamento e avaliação toxicológica in vivo de polissacarídeos sulfatados de Hypnea musciformis
Provedor de dados: 65
Autores: Rodrigues,José Ariévilo Gurgel; Araújo,Ianna Wivianne Fernandes de; Paula,Gabriela Almeida de; Vanderlei,Edfranck de Sousa Oliveira; Queiroz,Ismael Nilo Lino de; Quinderé,Ana Luíza Gomes; Coura,Chistiane Oliveira; Bessa,Érika Façanha; Lima,Ticiana de Brito; Benevides,Norma Maria Barros.
Objetivou-se isolar, fracionar e avaliar a toxicidade in vivo dos polissacarídeos sulfatados (PSs) da rodofícea Hypnea musciformis, quando obtidos por três métodos de extração (M I; M II e M III). Os PSs foram extraídos com papaína em tampão acetato de sódio 100mM (pH 5,0), contendo cisteína e EDTA (5mM) (M I) ou água (25-80°C (M II); 80°C (M III)) e, em seguida, determinados sua composição química de carboidratos totais, sulfato livre (SL) e proteínas contaminantes (PCs). Os PSs foram submetidos à cromatografia de troca iônica (DEAE-celulose) usando um gradiente de cloreto de sódio, sendo avaliado o grau de homogeneidade e densidade de carga por eletroforese em gel de agarose das frações obtidas e comparadas à heparina. O ensaio in vivo foi realizado em...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rodofícea; Polímeros sulfatados; Métodos de extração; Avaliação sistêmica.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000700017