Consulta: creatorFacets:"Bernard,E.A."
Registros recuperados: 2
Data/hora: 10/06/2024 05:42:42

Ectonucleotidase activities in Sertoli cells from immature rats
Provedor de dados: 56
Autores: Casali,E.A.; Silva,T.R. da; Gelain,D.P.; Kaiser,G.R.R.F.; Battastini,A.M.O.; Sarkis,J.J.F.; Bernard,E.A.
Sertoli cells have been shown to be targets for extracellular purines such as ATP and adenosine. These purines evoke responses in Sertoli cells through two subtypes of purinoreceptors, P2Y2 and P A1. The signals to purinoreceptors are usually terminated by the action of ectonucleotidases. To demonstrate these enzymatic activities, we cultured rat Sertoli cells for four days and then used them for different assays. ATP, ADP and AMP hydrolysis was estimated by measuring the Pi released using a colorimetric method. Adenosine deaminase activity (EC 3.5.4.4) was determined by HPLC. The cells were not disrupted after 40 min of incubation and the enzymatic activities were considered to be ectocellularly localized. ATP and ADP hydrolysis was markedly increased by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sertoli cells; Purine nucleotides; ATP diphosphohydrolase; Ecto-5'-nucleotidase; Ectoadenosine deaminase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000003

Retinol-induced changes in the phosphorylation levels of histones and high mobility group proteins from Sertoli cells
Provedor de dados: 56
Autores: Moreira,J.C.F.; Dal-Pizzol,F.; Rocha,A.B.; Klamt,F.; Ribeiro,N.C.; Ferreira,C.J.S.; Bernard,E.A.
Chromatin proteins play a role in the organization and functions of DNA. Covalent modifications of nuclear proteins modulate their interactions with DNA sequences and are probably one of the multiple factors involved in the process of switch on/off transcriptionally active regions of DNA. Histones and high mobility group proteins (HMG) are subject to many covalent modifications that may modulate their capacity to bind to DNA. We investigated the changes induced in the phosphorylation pattern of cultured Wistar rat Sertoli cell histones and high mobility group protein subfamilies exposed to 7 µM retinol for up to 48 h. In each experiment, 6 h before the end of the retinol treatment each culture flask received 370 KBq/ml [32P]-phosphate. The histone and HMGs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sertoli cells; Phosphorylation; Histones; High mobility group proteins; Retinol.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300005