Consulta: creatorFacets:"Berthelin, C. Heude"
Registros recuperados: 2
Data/hora: 29/05/2024 05:36:48

Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Akcha, Farida; Barranger, Audrey; Bachere, Evelyne; Berthelin, C. Heude; Piquemal, D.; Alonso, P.; Sallan, R. Rondon; Dimastrogiovanni, G.; Porte, C.; Menard, Dominique; Szczybelski, A.; Benabdelmouna, Abdellah; Auffret, M.; Rouxel, Julien; Burgeot, Thierry.
Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation...
Tipo: Text
Palavras-chave: Oyster; Pesticide; Reproduction; Histology; Gene expression; RT-qPCR; Steroid; Immunity.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00309/41983/83232.pdf

Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve,     Pecten maximus     (Linnaeus, 1758)
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Boonmee, A.; Berthelin, C. Heude; Kingtong, S.; Pauletto, M.; Bernay, B.; Adeline, B.; Suquet, Marc; Sourdaine, P.; Kellner, K.
In order to investigate the mechanisms of final maturation and capacitation of spermatozoa in Pecten maximus, we used a 2D proteomic approach coupled with MALDI-TOF/TOF mass spectrometry (MS) and bioinformatics search against the Pecten database, to set up a reference map of the proteome of spawned spermatozoa, and identified 133 proteins on the basis of the EST database. These proteins are mainly involved in energy production, ion and electron transport (44%), cell movement (22%) and developmental processes (10%). Comparison between proteomes of spermatozoa collected before and after transit through the genital ducts of P. maximus led to the identification of differentially expressed proteins. Most of them are associated with energy metabolism (aconitate...
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00353/46386/47178.pdf