Consulta: creatorFacets:"Binneck,Eliseu"
Registros recuperados: 9
Data/hora: 18/06/2024 17:21:20

Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems
Provedor de dados: Genetics and Molecular Biology
Autores: Martins,Polyana K.; Jordão,Berenice Q.; Yamanaka,Naoki; Farias,José R.B.; Beneventi,Magda A.; Binneck,Eliseu; Fuganti,Renata; Stolf,Renata; Nepomuceno,Alexandre L.
Drought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water-stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell division; Differential display; Gene expression; Real time PCR; Soybean; Water stress.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300019

An overall evaluation of the resistance (R) and pathogenesis-related (PR) superfamilies in soybean, as compared with Medicago and Arabidopsis
Provedor de dados: Genetics and Molecular Biology
Autores: Wanderley-Nogueira,Ana C.; Belarmino,Luis C.; Soares-Cavalcanti,Nina da M.; Bezerra-Neto,João P.; Kido,Ederson A.; Pandolfi,Valesca; Abdelnoor,Ricardo V.; Binneck,Eliseu; Carazzole,Marcelo F.; Benko-Iseppon,Ana M.
Plants have the ability to recognize and respond to a multitude of pathogens, resulting in a massive reprogramming of the plant to activate defense responses including Resistance (R) and Pathogenesis-Related (PR) genes. Abiotic stresses can also activate PR genes and enhance pathogen resistance, representing valuable genes for breeding purposes. The present work offers an overview of soybean R and PR genes present in the GENOSOJA (Brazilian Soybean Genome Consortium) platform, regarding their structure, abundance, evolution and role in the plantpathogen metabolic pathway, as compared with Medicago and Arabidopsis. Searches revealed 3,065 R candidates (756 in Soybean, 1,142 in Medicago and 1,167 in Arabidopsis), and PR candidates matching to 1,261 sequences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogen response; Biotic stress; Bioinformatics; Glycine max; Medicago truncatula.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200007

Genetic relationships between Chinese, Japanese, and Brazilian soybean gene pools revealed by simple sequence repeat (SSR) markers
Provedor de dados: Genetics and Molecular Biology
Autores: Yamanaka,Naoki; Sato,Hiroyuki; Yang,Zhenyu; Xu,Dong He; Catelli,Lizandra Lucy; Binneck,Eliseu; Arias,Carlos Alberto Arrabal; Abdelnoor,Ricardo Vilela; Nepomuceno,Alexandre Lima.
An understanding of the relationship of geographically different soybean gene pools, based on selectively neutral DNA markers would be useful for the selection of divergent parental cultivars for use in breeding. We assessed the relationships of 194 Chinese, 59 Japanese, and 19 Brazilian soybean cultivars (n = 272) using 12 simple sequence repeat (SSR) markers. Quantification Theory III and clustering analyses showed that the Chinese and Japanese cultivars were genetically quite distant to each other but not independent, while Brazilian cultivars were distantly related to the cultivars from the other two countries and formed a cluster that was distant from the other two gene pool clusters. Our results indicated that the Brazilian soybean gene pool is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA marker; Genetic relationship; Genetic resources; Glycine max.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100016

A web-based bioinformatics interface applied to the GENOSOJA project: databases and pipelines
Provedor de dados: Genetics and Molecular Biology
Autores: Nascimento,Leandro Costa do; Costa,Gustavo Gilson Lacerda; Binneck,Eliseu; Pereira,Gonçalo Amarante Guimarães; Carazzolle,Marcelo Falsarella.
The Genosoja consortium is an initiative to integrate different omics research approaches carried out in Brazil. Basically, the aim of the project is to improve the plant by identifying genes involved in responses against stresses that affect domestic production, like drought stress and Asian Rust fungal disease. To do so, the project generated several types of sequence data using different methodologies, most of them sequenced by next generation sequencers. The initial stage of the project is highly dependent on bioinformatics analysis, providing suitable tools and integrated databases. In this work, we describe the main features of the Genosoja web database, including the pipelines to analyze some kinds of data (ESTs, SuperSAGE, microRNAs, subtractive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Database; Gene expression; Soybean; Genosoja.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200002

Análise de RAPD na identificação de cultivares: uma metodologia útil?
Provedor de dados: Revista Brasileira de Sementes
Autores: Binneck,Eliseu; Nedel,Jorge Luiz; Dellagostin,Odir A.
Caracteres morfológicos têm sido utilizados tradicionalmente como assinaturas da identidade e pureza varietal e genética. Esses descritores se constituem em uma base pobre, por ser uma medida indireta da composição genética do material. Uma vez que caracteres moleculares revelam diferenças genéticas mais rapidamente, com maior precisão e sem o obscurecimento causado pelo efeito ambiental, oferecem vantagens significantes em termos de discriminação, confiabilidade, rapidez e custo reduzido. Um método molecular relativamente novo, DNA polimórfico amplificado ao acaso (RAPD), baseado na reação em cadeia da polimerase (PCR), tem sido adotado por alguns pesquisadores envolvidos no desenvolvimento de métodos de identificação de cultivares principalmente por ser...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA polimórfico amplificado ao acaso (RAPD); Reprodutibilidade; Marcadores moleculares; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100027

Análise numérica dos eletroforegramas de gliadinas de cultivares de triticale
Provedor de dados: Revista Brasileira de Sementes
Autores: Binneck,Eliseu; Nedel,Jorge Luiz; Dellagostin,Odir A; Peske,Silmar T; Barros,A.C.S.A.
Buscando automatizar o processo de identificação de cultivares pelo método de eletroforese de proteínas, o presente trabalho teve por objetivo avaliar a viabilidade da utilização de valores de intensidade das bandas como dados adicionais de polimorfismos na discriminação de cultivares e comparar o comportamento de vários coeficientes de similaridade na análise dos eletroforegramas de gliadinas. Os eletroforegramas de gliadinas de quatro cultivares de triticale estreitamente relacionadas foram comparados pela análise computacional utilizando seis coeficientes de similaridade binários (presença/ausência) (Jaccard, Sorensen-Dice, Nei &amp;amp; Li, Simple Matching, Yule e Baroni-Urbani) e cinco quantitativos (Pearson product moment correlation, Spearman,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coeficientes de similaridade; Identificação de cultivares; Eletroforese.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100044

Padrões eletroforéticos de hordeínas e isoenzimas para identificação de cultivares de cevada
Provedor de dados: Revista Brasileira de Sementes
Autores: Binneck,Eliseu; Nedel,Jorge Luiz; Dellagostin,Odir A; Barros,A.C.S.A; Peske,Silmar Teichert.
O objetivo deste trabalho foi determinar os padrões de eletroforese em gel de poliacrilamida das hordeínas e isoenzimas de cinco cultivares brasileiras de cevada (Hordeum vulgare L.): Embrapa 43, Embrapa 127, Embrapa 128, Embrapa 129 e BR 2, visando a identificação das cultivares. A uniformidade (homogeneidade) desses padrões também foi determinada por meio da análise individual de 90 amostras de sementes de cada cultivar. A maioria das cultivares foi prontamente discriminada pelos eletroforegramas de hordeínas. Três cultivares apresentaram padrões únicos de hordeínas, enquanto as outras duas (Embrapa 128 e BR 2) apresentaram padrões diferentes de esterases (EC 3.1.1.1). As medidas de similaridade calculadas a partir dos dados de hordeínas e esterases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Identificação de cultivares; PAGE; Prolaminas; Esterase; Hordeum vulgare.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100043

Pathogenicity, molecular characterization, and cercosporin content of Brazilian isolates of Cercospora kikuchii
Provedor de dados: Tropical Plant Pathology: fitopatologia brasileira
Autores: Almeida,Álvaro M. R.; Piuga,Fernanda F.; Marin,Silvana R. R.; Binneck,Eliseu; Sartori,Fábio; Costamilan,Leila M.; Teixeira,Maria R. O.; Lopes,Marcelo.
Cercospora kikuchii, involved with the defoliation of soybean (Glycine max) plants, is normally associated with Septoria glycines in late season. Seventy-two isolates from different regions in Brazil, obtained mainly from purple stained seeds, showed phenotypic variation. Cercosporin content and rate of colony growth was higly variable among isolates. A strong correlation between cercosporin content and virulence was identified. Genetic variation among and within populations was evaluated based on 86 RAPD loci. The RAPD analysis clustered all isolates into seven groups. No relationship was observed between RAPD groups and geographic origin or cercosporin content. The sequence of the internal spacer regions (ITS1-5.8S-ITS2) from 13 isolates chosen according...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotypic diversity; PCR-RFLP; RAPD; ITS sequence; Virulence.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000600005

Molecular cloning of αRYR hotspot region 1 from broiler chicken
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Ziober,Iris Lamberti; Paião,Fernanda Gonzales; Marin,Silvana Regina Rockenbach; Marchi,Denis Fabricio; Binneck,Eliseu; Nepomuceno,Alexandre Lima; Coutinho,Luiz Lehmann; Shimokomaki,Massami.
Samples of Pectoralis major m. were collected, and an RT-PCR analysis of the a-Ryanodine receptor (a RYR) from chicken mRNA hotspot region spanning aminoacid residues 386 to 540, numbered according to the turkey sequence, revealed two classes of transcripts. The sequences of the first class were similar to turkey and human with 97% and 74% of identity, respectively, and included all transcripts with substitutions in the nucleotide sequence. The second class was characterized by the deletion of nucleotides, leading to a premature stop codon and coding for a truncated and nonfunctional protein. These results are to date the first report related to the sequencing of the chicken αRYR hotspot region 1, which will possibility serve as a guide for further studies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ΑRYR; PSE; Chicken.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700029