Consulta: creatorFacets:"Birien, Tiphaine"
Registros recuperados: 2
Data/hora: 09/06/2024 05:37:31

Desulfurobacterium indicum sp nov., a thermophilic sulfur-reducing bacterium from the Indian Ocean
Provedor de dados: 5
Autores: Cao, Junwei; Birien, Tiphaine; Gayet, Nicolas; Huang, Zhaobin; Shao, Zongze; Jebbar, Mohamed; Alain, Karine.
A novel sulfur-reducing bacterium, strain K6013(T), was isolated from a sulfide sample collected at a depth of 2771 m from a high-temperature hydrothermal vent in the Indian Ocean. Cells were Gram-stain-negative, anaerobic, motile rods (0.92.2 x 0.4-0.6 mu m). The strain grew at NaCl concentrations ranging from 1 to 4.5% (w/v) (optimum 2.5 %), at pH 5 to 8 (optimum pH 6), and at temperatures between 40 and 75 degrees C (optimum 65 degrees C). K6013(T) was an obligate chemolithoautotroph, using thiosulfate, sulfur and nitrate as terminal electron acceptors in the presence of H-2 but not sulfate, sulfite nor nitrite. The major cellular fatty acids were C-16 : 0 (17.4 %), C-18 : 1 omega 7c/C-18 : 1 omega 6c (ummed feature 8, 37.91 %), C-18 : 0 (18.29 %) and...
Tipo: Text
Palavras-chave: Desulfurobacterium; Thermophilic; Hydrothermal vent; Chemolithoautotroph; Sulfur-reducer.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00395/50637/53724.pdf

Development of an Effective 6-Methylpurine Counterselection Marker for Genetic Manipulation in Thermococcus barophilus
Provedor de dados: 5
Autores: Birien, Tiphaine; Thiel, Axel; Henneke, Ghislaine; Flament, Didier; Moalic, Yann; Jebbar, Mohamed.
A gene disruption system for Thermococcus barophilus was developed using simvastatin (HMG-CoA reductase encoding gene) for positive selection and 5-Fluoroorotic acid (5-FOA), a pyrF gene for negative selection. Multiple gene mutants were constructed with this system, which offers the possibility of complementation in trans, but produces many false positives (&lt;80%). To significantly reduce the rate of false positives, we used another counterselective marker, 6-methylpurine (6-MP), a toxic analog of adenine developed in Thermococcus kodakarensis, consistently correlated with the TK0664 gene (encoding a hypoxanthine-guanine phosphoribosyl-transferase). We thus replaced pyrF by TK0664 on our suicide vector and tested T. barophilus strain sensitivity to 6-MP...
Tipo: Text
Palavras-chave: Archaea; Piezophiles; Hyperthermophiles; Genetics; Gene deletion; Deep sea; Hydrothermal vents.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00425/53679/54523.pdf