Consulta: creatorFacets:"Bouchez, Olivier"
Registros recuperados: 4
Data/hora: 02/06/2024 05:09:20

Transcriptomic analysis reveals specific metabolic pathways of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents
Provedor de dados: 5
Autores: Segura, Audrey; Bertoni, Marine; Auffret, Pauline; Klopp, Christophe; Bouchez, Olivier; Genthon, Clemence; Durand, Alexandra; Bertin, Yolande; Forano, Evelyne.
Background The cattle gastrointestinal tract (GIT) is the main enterohemorrhagic Escherichia coli (EHEC) reservoir. In order to identify nutrients required for the survival or multiplication of EHEC in the bovine GIT, we compared the transcriptomes of the EHEC O157:H7 reference strain EDL933 cultured in vitro in bovine digestive contents (DCs) (rumen, small intestine and rectum) using RNA-sequencing. Results Gene expression profiles showed that EHEC EDL933 activated common but also specific metabolic pathways to survive in the different bovine DCs. Mucus-derived carbohydrates seem important in EHEC nutrition in posterior DCs (small intestine and rectum) but not in rumen content. Additional carbohydrates (xylose, ribose, mannitol, galactitol) as well as...
Tipo: Text
Palavras-chave: EHEC O157:H7; Bovine gastrointestinal tract; RNA-seq; Metabolism; Carbon sources; Mucus-derived carbohydrates; Ethanolamine; Respiration.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00464/57563/59734.pdf

The contribution of ancient admixture to reproductive isolation between European sea bass lineages
Provedor de dados: 5
Autores: Duranton, Maud; Allal, Francois; Valiere, Sophie; Bouchez, Olivier; Bonhomme, Francois; Gagnaire, Pierre-alexandre.
Understanding how new species arise through the progressive establishment of reproductive isolation (RI) barriers between diverging populations is a major goal in Evolutionary Biology. An important result of speciation genomics studies is that genomic regions involved in RI frequently harbor anciently diverged haplotypes that predate the reconstructed history of species divergence. The possible origins of these old alleles remain much debated, as they relate to contrasting mechanisms of speciation that are not yet fully understood. In the European sea bass (Dicentrarchus labrax), the genomic regions involved in RI between Atlantic and Mediterranean lineages are enriched for anciently diverged alleles of unknown origin. Here, we used haplotype-resolved...
Tipo: Text
Palavras-chave: Ancient admixture; Introgression; Reproductive isolation; Genomic conflicts; Marine fish.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00636/74768/74920.pdf

Evolutionary processes and cellular functions underlying divergence in Alexandrium minutum
Provedor de dados: 5
Autores: Le Gac, Mickael; Metegnier, Gabriel; Chomerat, Nicolas; Malestroit, Pascale; Quere, Julien; Bouchez, Olivier; Siano, Raffaele; Destombe, Christophe; Guillou, Laure; Chapelle, Annie.
Understanding divergence in the highly dispersive and seemingly homogeneous pelagic environment for organisms living as free drifters in the water column remains a challenge. Here, we analyzed the transcriptome wide mRNA sequences, as well as the morphology of 18 strains of Alexandrium minutum, a dinoflagellate responsible for Harmful Algal Blooms worldwide, to investigate the functional bases of a divergence event. Analysis of the joint site frequency spectrum (JSFS) pointed toward an ancestral divergence in complete isolations followed by a secondary contact resulting in gene flow between the two diverging groups, but heterogeneous across sites. The sites displaying fixed SNPs were associated with a highly restricted gene flow and a strong...
Tipo: Text
Palavras-chave: Dinoflagellates; Harmful algal blooms; Populations Genomics; Pseudocryptic species; Speciation.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00347/45857/45529.pdf

Draft Genome Sequence of Enterohemorrhagic   Escherichia coli   O157:H7 Strain MC2 Isolated from Cattle in France
Provedor de dados: 5
Autores: Auffret, Pauline; Segura, Audrey; Bertin, Yolande; Klopp, Christophe; Bouchez, Olivier; Kérourédan, Monique; Bibbal, Delphine; Brugère, Hubert; Forano, Evelyne.
Enterohemorrhagic Escherichia coli (EHEC) with serotype O157:H7 is a major foodborne pathogen. Here, we report the draft genome sequence of EHEC O157:H7 strain MC2 isolated from cattle in France. The assembly contains 5,400,376 bp that encoded 5,914 predicted genes (5,805 protein-encoding genes and 109 RNA genes).
Tipo: Text
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00404/51538/52129.pdf