Consulta: creatorFacets:"Brígido,Luis Fernando de Macedo"
Registros recuperados: 2
Data/hora: 03/06/2024 16:16:12

High degree of concordance between ﬂow cytometry and geno2pheno methods for HIV-1 tropism determination in proviral DNA
Provedor de dados: 55
Autores: Torres,Alex José Leite; Brígido,Luis Fernando de Macedo; Abrahão,Marcos Herculano Nunes; Angelo,Ana Luiza Dias; Ferreira,Gilcivaldo de Jesus; Coelho,Luana Portes; Ferreira,João Leandro; Jorge,Célia Regina Mayoral Pedroso; Martins Netto,Eduardo; Brites,Carlos.
Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a ﬂow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identiﬁed by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identiﬁcation of X4 and R5 tropic viruses was attempted by geno2pheno...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Tropism; Flow cytometry; Geno2pheno.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200163

Association of X4 tropism with disease progression in antiretroviral-treated children and adolescents living with HIV/AIDS in São Paulo, Brazil
Provedor de dados: 55
Autores: Almeida,Flávia Jacqueline; Zaparoli,Mayra Simioni; Moreira,Denise Helena; Cavalcanti,Jaqueline de Souza; Rodrigues,Rosangela; Berezin,Eitan Naaman; Ferreira,João Leandro de Paula; Sáfadi,Marco Aurélio Palazzi; Brígido,Luis Fernando de Macedo.
Management of children with HIV/AIDS is specially challenging. Age-related issues do not allow for direct transposition of adult observations to this population. CXCR4 tropism has been associated with disease progression in adults. The geno2pheno web-base is a friendly tool to predict viral tropism on envelope V3 sequences, generating a false positive rate for a CXCR4 prediction. We evaluated the association of HIV-1 tropism prediction with clinical and laboratory outcome of 73 children with HIV/AIDS in São Paulo, Brazil. The CXCR4 tropism was strongly associated with a lower (nadir) CD4 documented during follow-up (p &lt; 0.0001) and with disease severity (clinical event and/or CD4 below 200 cells/mm3) at the last observation, using commonly applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Children; Viral tropism; Disease progression.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300300