Consulta: creatorFacets:"Brentano,L."
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Data/hora: 09/06/2024 05:59:21

A VP3/VP1 gene polymerase chain reaction assay for detection of chicken anemia virus in broiler samples
Provedor de dados: 45
Autores: Nogueira,E.O.; Brentano,L.; Ferreira,A.J.P.
A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region of the VP1 gene, for the diagnosis of CAV in organ samples of broiler flocks suspected of chicken infectious anemia. A comparison of the VP3/VP1 PCR with in vivo virus isolation revealed 100% agreement of the results, with 13 positive and 3 negative samples in both assays, indicating that the VP3/VP1 PCR is a specific diagnostic method. Tissues from additional 24 broiler chicken flocks, with CAV-like lesions and clinical history were then tested only by the VP3/VP1 PCR and a reference PCR with published primers for the VP1 gene. Nineteen samples resulted positive and one negative in both PCR, while another 4 samples were positive only in the VP3/VP1 PCR. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Chicken anemia virus; CAV; PCR; Diagnostic test.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800001