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Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
Provedor de dados: Biological Research
Autores: CÓRDOVA,JOSÉ LUIS; ASTORGA,JOSEFA; SILVA,WALLY; RIQUELME,CARLOS.
Between November 1997 and April 1998, several human gastroenteritis cases were reported in Antofagasta, a city in the north of Chile. This outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as Vibrio parahaemolyticus. This was the first report of this bacterium causing an epidemic in Chile. V. parahaemolyticus was the only pathogenic bacterium isolated from patient stools and from shellfish samples. These isolates were analyzed by polymerase chain reaction (PCR) amplification of the pR72H gene, a species-specific sequence. Based on the pR72H gene amplification pattern, at least three different isolates of V. parahaemolyticus were found. Two isolates (named amplicons A and C) generated PCR products...
Tipo: Journal article
Palavras-chave: Bacterial bloom; Bacterial geographical dispersion; PCR amplification; Molecular typification.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300017