Consulta: creatorFacets:"Cahu, Chantal"
Registros recuperados: 59
Data/hora: 03/06/2024 18:27:20

Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): Comparison with salmonid species and analysis of CpG methylation
Provedor de dados: 5
Autores: Geay, Florian; Zambonino, Jose-luis; Reinhardt, Richard; Kuhl, Heiner; Santigosa, Ester; Cahu, Chantal; Mazurais, David.
Marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (HUFA) in comparison to strict freshwater and anadromous species. It is admitted that the Delta(6) desaturase (FADS2) is a key enzyme in the HUFA biosynthetic pathway. We investigated by quantitative PCR the relative amounts of FADS2 mRNA in European sea bass (Dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (Oncorhynchus mykiss L.). The analysis of the expression data was performed regarding the difference of the characteristics of a critical fragment of the fads2 gene promoter between sea bass and Atlantic salmon. The lower level of fads2 gene expression observed in sea bass suggested that fads2 gene putative promoter, which exhibited an...
Tipo: Text
Palavras-chave: FADS2; Sea bass; Gene expression; Promoter; Methylation.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00065/17621/15310.pdf

Compte-rendu de mission en Tunisie (13-17 Juin 2005). Atelier de travail sur les nouvelles perspectives de la recherche scientifique en aquaculture
Provedor de dados: 5
Autores: Thouard, Emmanuel; Buchet, Vincent; Cahu, Chantal; Coeurdacier, Jean-luc; Elie, Pierre; Gerard, André; Hussenot, Jerome; Legendre, Marc; Prou, Jean; Robert, Rene.
Les missions réalisées fin 2004 et début 2005 ont permis de faire le point sur les coopérations potentielles entre l'INSTM et l'IFREMER. Un certain nombre de domaines de coopération et de sujets de collaboration ont été identifiés et se sont traduits (ou se traduiront) par des actions de coopération (missions, stages, formations) soutenues financièrement par le SCAC de l'Ambassade de France à Tunis. La coopération entre l'IFREMER et l'INSTM dans le domaine de l'aquaculture a été très active dans le cadre du programme« Aquaculture 2001 » (1997-2001). Depuis cette période les relations se sont limitées à la préparation du programme DORAD qui était la suite d' «Aquaculture 2001 » . En l'absence du financement FSP sollicité pour DORAD, les deux organismes ont...
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00305/41648/40857.pdf

Intake of high levels of vitamin A and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in European sea bass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Villeneuve, Laure; Gisbert, Enric; Moriceau, Jacques; Cahu, Chantal; Zambonino, Jose-luis.
The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13 d post-hatching (dph); period B: from 13 to 18 dph; period C: from 18 to 23 dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13 dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades,...
Tipo: Text
Palavras-chave: Retinoid pathway; Morphogenesis; Vitamin A; PUFA; Sea bass larvae.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1215.pdf

Dietary probiotic live yeast modulates antioxidant enzyme activities and gene expression of sea bass (Dicentrarchus labrax) larvae
Provedor de dados: 5
Autores: Tovar-ramirez, D.; Mazurais, David; Gatesoupe, J. F.; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, J. L.
The main goal of this work was to determine the effect of dietary live yeast Debaryomyces hansenii on the enzymatic antioxidative status of sea bass Dicentrarchus labrax larvae. Growth, activity and expression of the main antioxidative enzymes: catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD), and heat shock protein (HSP70) were measured in sea bass larvae at 23 and 48 days after hatching. Larvae were fed on two microdiets: group one, fed microdiet containing live yeast and the control group fed microdiet without yeast. Heat shock protein 70 showed the same expression levels in both fish larvae fed yeast and the control diet. The group fed D. hansenii showed highest growth and lower activity and expression levels of GPX and SOD...
Tipo: Text
Palavras-chave: Antioxidant enzymes; Probiotics; Debaryomyces hansenii; Dicentrarchus labrax.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11299/7881.pdf

Dietary lipid level affects fatty acid composition and hydrolase activities of intestinal brush border membrane in seabass
Provedor de dados: 5
Autores: Cahu, Chantal; Zambonino, Jose-luis; Corraze, Genevieve; Coves, Denis.
Triplicate groups of juvenile seabass (initial weight of 241 g) were fed during 13 weeks three isonitrogenous experimental diets containing different lipid levels, 12% (LL group), 21% (ML group) and 30% (HL group). At the end of the experiment, fish weight gain was similar among the 3 dietary groups. Intestinal brush border membranes were purified for each dietary group; one part of the brush border fraction was dedicated to enzyme assays, the remaining fraction being used for lipid extraction followed by fatty acid analysis. The fatty acid composition of the brush border membrane differed among the 3 groups, although the 3 experimental diets had the same fatty acid composition. The increase in dietary lipid level resulted in a lowering in (n-3)...
Tipo: Text
Palavras-chave: Membrane lipid; Intestine; Fish; Fatty acids; Dietary lipids; Brush border hydrolases.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-901.pdf

Digestive enzyme activity and food ingesta in juvenile shrimp Litopenaeus vannamei (Boone, 1931) as a function of body weight
Provedor de dados: 5
Autores: Gamboa Delgado, Julián; Molina Poveda, César; Cahu, Chantal.
A study was conducted to evaluate variations of digestive enzyme activities in Litopenaeus vannamei (Boone) reared in commercial ponds under semi-intensive conditions. Shrimp were collected at each body weight increase of 2 g. As the shrimp grew (2-12 g), significant increases in the activities of lipase and chymotrypsin were observed. The total protease activity decreased from 6 g onwards. Trypsin activity showed a peak at 6 g and amylase activity increased two-fold after 2 g. Additionally, the stomach contents were analysed microscopically for shrimp between 2 and 10 g. Plant matter contributed above 30% of the total stomach content in 6-, 8- and 10-g shrimp. Detritus represented 58% and 62% of the total stomach content in 2- and 4-g shrimp,...
Tipo: Text
Palavras-chave: Semi intensive culture; Stomach content; Digestive enzymes; Litopenaeus vannamei; Shrimp.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-902.pdf

Probiotic treatment of live food organisms for Atlantic bluefin tuna larvae: microbiological &amp; immunological criteria
Provedor de dados: 5
Autores: Coves, Denis; De Vogue, Benoist; Desbruyeres, Elisabeth; Dhormes, Benoit; Fievet, Julie; Huelvan, Christine; Lallement, Stephane; Le Gall, Marie-madeleine; Ruelle, Francois; Vidal, Marie-odile; Castex, M.; Mazurais, David; Cahu, Chantal; Gatesoupe, François-joel.
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00051/16189/13702.pdf

Domestication and nutrition in fish
Provedor de dados: 5
Autores: Cahu, Chantal.
The aim of this paper is to evaluate to what extent, the study of feeding regimes of fish could be valuable to indicate the possibilities and the constraints of their domestication. Though the natural feeding regime of fish seems to be disadvantageous for their domestication, as protein requirement is high, different species could be considered as domesticated. These species are herbivorous, detritivorous, omnivorous as well as carnivorous. In the same way, there are large differences in gut anatomy, but domestication concerns species with or without stomach, and species with short or very long intestine. Nutritional requirements do not allow predicting species that could be domesticated. Some species seem to be able to use vegetable protein in...
Tipo: Text
Palavras-chave: TROUT ONCORHYNCHUS MYKISS; TURBOT PSETTA MAXIMA; RAINBOW TROUT; TOTAL REPLACEMENT; FLESH QUALITY; LIVE FOOD; DIET; PROTEIN; GROWTH; LARVAE.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00000/11021/7571.pdf

Expression and activities of pancreatic enzymes in developing sea bass larvae (Dicentrarchus labrax) in relation to intact and hydrolyzed dietary protein; involvement of cholecystokinin
Provedor de dados: 5
Autores: Cahu, Chantal; Ronnestad, I; Grangier, V; Zambonino, Jose-luis.
In order to assess the influence of dietary protein on digestive enzyme expression and cholecystokinin (CCK) content in sea bass larvae, four groups of larvae were fed experimental diets from mouth opening until day 42: three isonitrogenous diets with increasing protein hydrolysate levels (0%, 14% and 46% of crude matter) and one diet incorporating starch. The groups fed high starch or high protein hydrolysate level exhibited the lowest growth. The final weight in these groups was 9.5 and 5.6 mg, respectively, whereas it reached approximately 20.0 mg in the groups fed 0% or 14% protein hydrolysate level. The highest levels of trypsin secretion were observed in larvae fed the lowest protein hydrolysate level. Paradoxically, the groups fed diet containing...
Tipo: Text
Palavras-chave: Starch; Sea bass larvae; Protein hydrolysate; Pancreatic enzyme secretion; Cholecystokinin.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-442.pdf

Cloning, Tissue Expression Analysis, and Functional Characterization of Two Δ6-Desaturase Variants of Sea Bass (Dicentrarchus labrax L.)
Provedor de dados: 5
Autores: Santigosa, Ester; Geay, Florian; Tonon, Thierry; Le Delliou, Herve; Kuhl, Heiner; Reinhardt, Richard; Corcos, Laurent; Cahu, Chantal; Zambonino, Jose-luis; Mazurais, David.
Fish are the main source of the n-3 highly unsaturated fatty acids, which are crucial for human health. Their synthesis from C18 precursors is mediated by desaturases and elongases, but the activity of these enzymes has not been conclusively established in marine fish species. This study reports the cloning, tissue expression, and functional characterization of a sea bass (Dicentrarchus labrax L.) Δ6-desaturase and one of its splicing variants. Two cDNAs with open reading frames of 1,346 and 1,354 bp were cloned and named D6D and D6D-V, respectively. Both deduced protein sequences (445 and 387 amino acids, respectively) contained two transmembrane regions and the N-terminal cytochrome b5 domain with the HPGG motif characteristic of microsomal desaturases....
Tipo: Text
Palavras-chave: Sea bass (Dicentrarchus labrax); Desaturase; HUFA biosynthesis; Fish; EPA.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00023/13470/10477.pdf

The Digestive Function of Pseudoplatystoma punctifer Early Juveniles Is Differentially Modulated by Dietary Protein, Lipid and Carbohydrate Content and Their Ratios
Provedor de dados: 5
Autores: Castro-ruiz, Diana; Andree, Karl B.; Solovyev, Mikhail M.; Fernández-méndez, Christian; García-dávila, Carmen; Cahu, Chantal; Gisbert, Enric; Darias, Maria J.
Pseudoplatystoma punctifer is an Amazonian catfish highly appreciated for its high flesh quality, size, and commercial value. Its aquaculture is pursued to satisfy the demands of an increasing population in the region. However, knowledge of the nutritional needs during the early life stages is necessary for improving growth and reducing the incidence of cannibalism, factors that limit the success of its commercial farming. This study aimed at evaluating the influence of four diets containing different protein and lipid levels (30:15, 30:10, 45:15, or 45:10 in %) in the digestive physiology and performance of early juveniles. The results showed that the dietary protein:lipid as well as carbohydrate levels and ratios influenced differently the whole-body...
Tipo: Text
Palavras-chave: Neotropical fish; Aquaculture; Digestive enzymes; Gene expression; Nutrition; Juveniles; Histology; Development; Macronutrients; Diet.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00678/79019/81420.pdf

Dietary modulation of some digestive enzymes and Metabolic processes in developing marine fish: Applications to diet formulation
Provedor de dados: 5
Autores: Zambonino, Jose-luis; Cahu, Chantal.
During these last 20 years, many studies have focussed on the development of the digestive tract in marine fish larvae. Most of the studies aimed at acquiring knowledge on the optimal form of dietary supply for different nutrients, in order to formulate a compound diet able to totally replace live preys in the fish larvae feeding sequence. Consequently, most of the studies aimed at describing the effect of dietary adaptation on digestive enzymes profile, the morphology of the main organs, while others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport...) were poorly investigated.   This review reports the more recent data on dietary modulation of digestive enzymes with a particular emphasis, when possible, on the...
Tipo: Text
Palavras-chave: Nuclear receptors; Digestive enzymes; Fatty acids; Vitamins; Dietary proteins and lipids; Fish larvae.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3045.pdf

Influence of dietary phospholipids on early ontogenesis of fish
Provedor de dados: 5
Autores: Cahu, Chantal; Gisbert, Enric; Villeneuve, Laure A. N.; Morais, Sofia; Hamza, Neila; Wold, Per-avid; Zambonino, Jose-luis.
The aim of this paper is to provide explanations of how dietary phospholipid (PL) globally improves fish larval development, including growth and survival, digestive functions and skeletal development, and to propose optimal PL levels and sources in fish larval diets. Dietary incorporation of 8-12% PL related to dry matter (d.m.) promotes growth and enhanced survival in various species. Marine source PL, incorporating highly unsaturated fatty acids, was most efficient than soybean lecithin. This beneficial effect was explained by an enhancement in digestive functions, assessed by digestive enzyme activities and histomorphology. Nevertheless, 1.5-2.5% highly unsaturated fatty acids related to diet d.m. supplied by PL improved growth, survival and skeletal...
Tipo: Text
Palavras-chave: Gene regulation; Skeletal development; Digestive functions; Fish larvae; Phospholipid.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6460.pdf

Comparison of growth, digestive system maturation and skeletal development in sea bass larvae reared in an intensive or a mesocosm system
Provedor de dados: 5
Autores: Zouiten, Dora; Ben Khemis, Ines; Masmoudi, Ahmed Slaheddin; Huelvan, Christine; Cahu, Chantal.
The quality of development in intensive or mesocosm hatchery-reared Dicentrarchus labrax larvae was investigated using physiological indicators assessing ontogeny. Larvae were reared in intensive (120 L tanks) and in mesocosm systems (20 m(3) enclosures) with the same feeding sequence, excluding the wild zooplankton naturally available in mesocosms. Faster growth was recorded since early development [16 day after hatching (DAH)] in the mesocosm. Maturation of the digestive system also occurred earlier as indicated by the higher amylase secretion ratios, the intestinal maturation index (alkaline phosphatase/leucine-alanine peptidase and aminopeptidase-N/leucine-alanine peptidase ratios) and the more developed intestinal epithelium at 23 DAH. Nevertheless,...
Tipo: Text
Palavras-chave: Fish larvae; Hatchery; Mesocosm; Ontogenesis; Enzyme; Skeletogenesis.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00051/16225/14064.pdf

Ontogeny of the digestive enzyme activity of the Amazonian pimelodid catfish Pseudoplatystoma punctifer (Castelnau, 1855)
Provedor de dados: 5
Autores: Castro-ruiz, Diana; Mozanzadeh, Mansour Torfi; Fernández-méndez, Christian; Andree, Karl B.; García-dávila, Carmen; Cahu, Chantal; Gisbert, Enric; Darias, Maria J.
The aim of the study was to evaluate the functional ontogeny of the digestive system of Pseudoplatystoma punctifer through the analysis of the activity of the main intestinal (alkaline phosphatase, aminopeptidase N, maltase and leucine-alanine peptidase), pancreatic (trypsin, chymotrypsin, total alkaline proteases, bile-salt activated lipase and amylase) and gastric (pepsin) enzymes. Larvae were raised in triplicate in a recirculation system from 4 to 27 days post fertilization (dpf) at an initial density of 90 larvae L−1, 27.8 ± 0.7 °C and 0 L: 24D photoperiod. Larvae were fed from 4 to 17 dpf with Artemia nauplii and weaned onto an experimentally formulated feed (crude protein content ~ 45%; crude fat content ~ 10%; crude carbohydrate ~ 8%) within...
Tipo: Text
Palavras-chave: Pseudoplatystoma punctifer; Ontogeny; Enzymatic activity; Digestive system; Brush border; Pancreatic enzymes; Gastric enzymes.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00479/59082/61723.pdf

Activities of selected digestive enzymes during larval development of large yellow croaker (Pseudosciaena crocea)
Provedor de dados: 5
Autores: Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen.
Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,...
Tipo: Text
Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf

Dietary neutral lipid level and source in marine fish larvae: Effects on digestive physiology and food intake
Provedor de dados: 5
Autores: Morais, Sofia; Conceicao, L; Ronnestad, I; Koven, W; Cahu, Chantal; Zambonino, Jose-luis; Dinis, M.
A growth depressing effect of high dietary neutral lipid levels in marine fish larvae has been reported. This may be a result of a decrease in the efficiency or activity of digestive enzymes, a reduction in absorption efficiency and/or a decrease in food intake. The present work reviews recent studies carried out on commercially valuable species (Atlantic herring, Senegalese sole, European seabass and gilthead seabream) that investigated the effects of neutral lipid level and lipid source (fatty acid composition) on some of these key factors influencing larval growth. The results seem to collectively indicate that lipid transport from the enterocytes into the body may be more problematic in larval stages dealing with high neutral lipid diets than lipolytic...
Tipo: Text
Palavras-chave: Ingestion; Lipid droplets; Digestion; Absorption; Fatty acid composition; Lipid quantity.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3029.pdf

Ontogeny and physiology of the digestive system of marine fish larvae
Provedor de dados: 5
Autores: Zambonino, Jose-luis; Gisbert, E; Sarasquete, C; Navarro, I; Gutiérrez, J; Cahu, Chantal.
The ontogeny of the digestive tract of marine fish larvae has been the subject of many studies these last twenty years. Beyond the scientific interest for such animal species, particularly for developmental aspects, most of these studies aimed to come up with the expectations of commercial hatcheries by reducing the bottlenecks in larvae culture and weaning processes (switch from live preys to compound diet feeding sequence). Consequently, the profile and dietary adaptation of digestive enzymes, the description and histology of the main organs have been extensively studied, when others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport…) were poorly investigated and this imbalanced knowledge is showed through this...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00086/19684/17307.pdf

Reduced lipid intake leads to changes in digestive enzymes in the intestine but has minor effect on key enzymes of hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S.
For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced...
Tipo: Text
Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf

Double staining protocol for developing European sea bass (Dicentrarchus labrax) larvae
Provedor de dados: 5
Autores: Darias, M. J.; Wing, O. Lan Chow; Cahu, Chantal; Zambonino, Jose-luis; Mazurais, David.
P&gt;The alcian blue-alizarin red technique was successfully adjusted to stain developing European sea bass (Dicentrarchus labrax) larvae. For an optimal staining protocol design both larval size and their morphological characteristics at each developmental stage were considered, since such parameters notably influence the staining of tissues. The incubation times of the different solutions were adjusted to allow the stain penetration for revealing the integrity of cartilaginous and bony tissues without significant tissue degradation. Three developmental windows were determined for an optimal staining procedure: (i) 4.5-6.4 mm, (ii) 6.7-8.7 mm, and (iii) 12.8-15.5 mm total length (TL). In order to validate the continuity of staining along the larval...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11371/8364.pdf

Do protein hydrolysates improve survival and growth of newly-hatched spotted, wolffish (Anarhichas minor), a non-metamorphic aquaculture fish species?
Provedor de dados: 5
Autores: Savoie, A; Le Francois, Nathalie; Cahu, Chantal; Blier, P; Andreassen, I.
Despite larval robustness characterized by the absence of metamorphosis and readiness for exogenous feeding based on commercial feed, the spotted wolffish (Anarhichas minor) displays highly variable survival at first-feeding. In this study, we investigated the use of three dietary concentrations of protein hydrolysates (PH, pre-digested proteins) (0, 10 and 20%) when newly hatched juvenile wolffish were held at three different rearing temperatures namely, 5, 8 and 12 degrees C to determine whether digestion of protein was a limiting factor for fish growth and survival. Final weights for fish at 5, 8,and 12 degrees C at day 60 were respectively 0.35, 1.19 and 2.02 g. Mean specific growth rates were 1.97, 4.01 and 4.88%/day and survival rates were 49.8, 53.4...
Tipo: Text
Palavras-chave: Survival; Growth; First feeding; Protein hydrolysates; Spotted wolffish; Anarhichas minor.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2141.pdf

Biofloc contribution to antioxidant defence status, lipid nutrition and reproductive performance of broodstock of the shrimp Litopenaeus stylirostris: Consequences for the quality of eggs and larvae
Provedor de dados: 5
Autores: Cardona, Emilie; Lorgeoux, Benedicte; Chim, Liet; Goguenheim, Jean; Le Delliou, Herve; Cahu, Chantal.
The aim of this study was to determine biofloc contributions to the antioxidant status and lipid nutrition of broodstock of Litopenaeus stylirostris in relationship with their reproductive performance and the health of larvae produced. Shrimp broodstock reared with Biofloc technology (BFT) compared to Clear water (CW) exhibited a higher health status with (i) a better final survival rate during the reproduction period (52.6% in CW against 79.8% in BFT); (ii) higher glutathione level (GSH) and total antioxidant status (TAS), reduced oxidized/reduced glutathione ratio and a higher spawning rate and frequency as well as higher gonado-somatic index and number of spawned eggs. Finally, larvae from broodstock from BFT exhibited higher survival rates at the Zoe 2...
Tipo: Text
Palavras-chave: Biofloc technology; Shrimp broodstock culture; Litopenaeus stylirostris; Reproductive performance; Antioxidant defences; Fatty acids; Larval survival.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00275/38618/37148.pdf

A histological study on the development of the digestive system of Pseudosciaena crocea larvae and juveniles
Provedor de dados: 5
Autores: Mai, Kangsen; Yu, Hairui; Ma, Hongming; Duan, Qingyuan; Gisbert, Enric; Zambonino, Jose-luis; Cahu, Chantal.
The ontogenetic development of the gut and accessory organs in large yellow croaker Pseudosciaena crocea was investigated using light microscopy from hatching up to the juvenile stage (40 days post hatch, dph). At 3 dph (mean +/- S.D., 4.1 +/- 0.1 mm total length, L-T), coinciding with the buccopharynx opening, larvae started to feed exogenously, and the gut consisted of a well-developed buccopharynx, a partially-differentiated oesophagus and an intestine divided in three regions (anterior intestine, intermediate intestine and rectum). Yolk reserves were not completely depleted at the onset of exogenous feeding, and a period of mixed nutrition was observed up to 6 dph (4.3 +/- 0.1 mm L-T) when yolk was definitively exhausted. Important morphological...
Tipo: Text
Palavras-chave: Pseudosciaena crocea; Ontogenetic development; Large yellow croaker; Histology; Digestive system.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-930.pdf

Substitution of live food by formulated diets in marine fish larvae
Provedor de dados: 5
Autores: Cahu, Chantal; Zambonino, Jose-luis.
Until recently, it was considered impossible to feed newly hatched marine fish species with a compound diet. Substituting a compound diet for live prey was performed several weeks after hatching, depending on the species. Compound diets were well ingested at the early stage but larvae died with a gut full of food, suggesting that larvae were unable to digest the compound diet. The hypothesis was that younger larvae have insufficient digestive enzymes to thrive on compound diets, and that exogenous enzymes, provided from live prey, are necessary for early stages.The organogenesis of marine fish larvae is not completely achieved at hatching and histological studies have revealed that the anatomy of the digestive tract undergoes developmental changes over...
Tipo: Text
Palavras-chave: Protein; Phospholipid; Pancreatic enzymes; Microparticulate diet; Intestinal enzymes; Protein hydrolysate; Gut maturation; Fish larvae.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-447.pdf

Effects of the total replacement of fish-based diet with plant-based diet on the hepatic transcriptome of two European sea bass (Dicentrarchus labrax) half-sibfamilies showing different growth rates with the plant-based diet
Provedor de dados: 5
Autores: Geay, Florian; Ferraresso, Serena; Zambonino, Jose-luis; Bargelloni, Luca; Quentel, Claire; Vandeputte, Marc; Kaushik, Sachi; Cahu, Chantal; Mazurais, David.
Background: Efforts towards utilisation of diets without fish meal (FM) or fish oil (FO) in finfish aquaculture have been being made for more than two decades. Metabolic responses to substitution of fishery products have been shown to impact growth performance and immune system of fish as well as their subsequent nutritional value, particularly in marine fish species, which exhibit low capacity for biosynthesis of long-chain poly-unsaturated fatty acids (LC-PUFA). The main objective of the present study was to analyse the effects of a plant-based diet on the hepatic transcriptome of European sea bass (Dicentrarchus labrax). Results: We report the first results obtained using a transcriptomic approach on the liver of two half-sibfamilies of the European sea...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00056/16703/14167.pdf

Phospholipids vs. neutral lipids: Effects on digestive enzymes in Atlantic cod (Gadus morhua) larvae
Provedor de dados: 5
Autores: Wold, P; Hoehne Reitan, K; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J; Kjorsvik, E.
The aim of this study was to evaluate the effect of dietary lipid classes (phospholipid vs. neutral lipid) and level of n-3 highly unsaturated fatty acids (HUFAs) on growth, survival and digestive enzymatic activity in Atlantic cod (Gadus morhua) larvae. Larvae were fed enriched rotifers from mouth opening and were weaned to formulated diets during a co-feeding period lasting from 17 days post hatch (dph) to 24 dph. Larvae were fed exclusively compound diets from 24 dph until the end of the experiment. Three isoproteic and isolipidic compound diets with gradually decreasing levels of DHA and EPA in the dietary phospholipid fraction (diet PL3 &gt;PL1 &gt;NL1) and increasing levels of total dietary neutral lipids (PL3 &lt;PL1 &lt;NL1) were used. Larvae fed...
Tipo: Text
Palavras-chave: Gut maturation; Digestive enzymes; Phospholipids; Early weaning; Atlantic cod larvae.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3801.pdf

Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae
Provedor de dados: 5
Autores: Kotzamanis, Y. P.; Gisbert, E.; Gatesoupe, Joel; Zambonino, Jose-luis; Cahu, Chantal.
Two Fish Protein Hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular weight of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae...
Tipo: Text
Palavras-chave: Pseudoalteromonas Bacillus Vibrio; Marinomonas Bacterial challenge microbiota Fish hydrolysate Digestive enzymes Development Fish larvae.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2006/publication-2145.pdf

Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development
Provedor de dados: 5
Autores: Tovar Ramirez, Dariel; Zambonino-infante, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R.
Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA...
Tipo: Text
Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-831.pdf

Dietary protein hydrolysate and trypsin inhibitor effects on digestive capacities and performances during early-stages of spotted wolffish: Suggested mechanisms
Provedor de dados: 5
Autores: Savoie, A.; Le Francois, N. R.; Lamarre, S. G.; Blier, P. U.; Beaulieu, L.; Cahu, Chantal.
Growth rate is dependent upon adequate provision of amino acids especially in newly-hatched fish which experience very high growth rate. The replacement of a fraction of protein content by partially hydrolyzed (pre-digested) proteins was carried out and the digestive capacities and performances of larval/juvenile spotted wolffish (Anarhichas minor) were measured. The goal of this study was to verify whether the scope for growth is principally dictated by the proteolytic capacity of the digestive system by examining the effect of protein hydrolysates (PH) and trypsin inhibitor dietary inclusion on protein digestion/assimilation capacities, growth and survival. Four experimental diets were examined: C (control) I (supplemented with 750mg/kg soybean trypsin...
Tipo: Text
Palavras-chave: Spotted wolffish; Anarhichas minor; Early-stages; Trypsin inhibitor; Protein hydrolysate; Digestive capacity; SBTI.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14399/12088.pdf

Effect of lipid level in a compound diet on the development of red drum (Sciaenops ocellatus) larvae
Provedor de dados: 5
Autores: Buchet, Vincent; Zambonino, Jose-luis; Cahu, Chantal.
Red drum larvae were fed from day 11 to day 24 post-hatch, with Artemia (LP group) or two isoproteinic-formulated diets with varying lipid levels, 15% (L15 group) and 30% (L30 group). The lipid fraction was composed of a mixture of cod liver oil and soy lecithin. Larvae fed Artemia exhibited the greatest length, L30 diet induced a larvae length significantly greater than that obtained with L15 diet (P &lt; 0,05). No difference was observed in larvae survival among the three dietary groups. The increase in dietary lipid concentration led to an increase in amylase and trypsin secretion levels in 24-day-old larvae. The pancreatic secretion level measured in L30 group was close to that of LP group, suggesting that the maturation of the pancreatic functions was...
Tipo: Text
Palavras-chave: Gut maturation; Digestive enzymes; Compound diet; Lipid; Larvae; Red drum.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-503.pdf

Metabolic and digestive activity profiles of newly hatched spotted wolffish (Anarhichas minor Olafsen): effect of temperature
Provedor de dados: 5
Autores: Savoie, A; Le Francois, N; Cahu, Chantal; Blier, P.
Three groups of newly hatched spotted wolffish (Anarhichas minor) were held at three different temperatures in order to determine relationships between metabolic, digestive and growth response in rapidly developing larvae. Growth rates were successfully modulated by temperature (5, 8 and 12 degrees C). Activity levels of trypsin and lactate dehydrogenase (LDH) were positively linked to specific growth rates at all temperatures. Trypsin showed a positive compensation (higher activity at lower temperature) whereas glycolytic enzymes (pyruvate kinase and Lactate dehydrogenase) and aspartate aminotransferase (AST) showed a negative compensation (lower activity at lower temperature). Citrate synthase was not affected by growth rate, indicating that the level of...
Tipo: Text
Palavras-chave: Anarhichas minor; Temperature; Growth; Energy metabolism; Digestive capacities.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3810.pdf

Allometric growth patterns and development in larvae and juveniles of thick-lipped grey mullet Chelon labrosus reared in mesocosm conditions
Provedor de dados: 5
Autores: Ben Khemis, Ines; Gisbert, Enric; Alcaraz, Carles; Zouiten, Dora; Besbes, Raouf; Zouiten, Amina; Masmoudi, Ahmed Slaheddine; Cahu, Chantal.
Allometric growth and ontogeny were studied in thick-lipped grey mullet Chelon labrosus reared in mesocosms from 1 to 71 day post hatching (dph). Multivariate allometric analysis of morphometric growth distinguished three distinct developmental stanzas separated by two morphometric metamorphosis lengths (L-m1=4.46 +/- 0.06mm; L-m2= 28.56 +/- 1.04mm). Body mass growth also showed three distinct episodes separated by two inflections, correlated with morpho-functional changes. First episode concerned pre-flexion larvae and ended around 4.5mm-L-T (14-dph), coinciding with estimated L-m1. It was distinguished by reduced growth, but intense morphogenesis and differentiation processes. Organogenesis and allometric changes indicated that development priorities...
Tipo: Text
Palavras-chave: Thick-lipped grey mullet; Chelon labrosus; Larval development; Allometric growth; Mesocosm.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00166/27742/26425.pdf

Dietary TAG source and level affect performance and lipase expression in larval sea bass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Morais, Sophia; Cahu, Chantal; Zambonino-infante, Jose-luis; Robin, Jean; Ronnestad, I; Dinis, M; Conceicao, L.
The influence of dietary TAG source (fish oil, triolein, and coconut oil) and level (7.5 and 15% of the diet) on growth, lipase activity, and mRNA level was studied in sea bass larvae, from mouth opening until day 24 and from day 37 to 52. Fish oil and triolein induced better growth in both experiments, this being significant at a higher dietary level. Coconut oil significantly decreased growth at the higher level, possibly as the result of an excessive supply of medium-chain TAG. Growth was not related to lipase specific activity, suggesting a production in excess to dietary needs. Body lipid content was positively related to dietary lipid level and was affected by lipid quality. In addition, larval FA composition generally reflected that of the diet. The...
Tipo: Text
Palavras-chave: Lipase activity; Dietary; Growth; Larvae; Sea bass; Fish culture.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-785.pdf

Cloning of endothelin-1 (ET-1) from European sea bass (Dicentrarchus labrax) and its gene expression analysis in larvae with retinoic acid-induced malformations
Provedor de dados: 5
Autores: Geay, Florian; Darias, Maria J.; Santigosa, E.; Desbruyeres, Elisabeth; Quazuguel, Patrick; Zambonino, Jose-luis; Cahu, Chantal; Mazurais, David.
It is known in vertebrates that endothelin-1 (ET-1) plays a key role ill morphogenesis whose expression level greatly influences the development of craniofacial malformations. In the Present study, the complete cDNA fragment encoding a precursor of endothelin-1, the preproendothelin-1 (PPET1), was cloned by RACE-PCR from European sea bass. The cDNA encoded a 199 amino acid polypeptide that was composed of the "mature" and "big" ET-1. Relative ET-1 expression levels were investigated in European sea bass larvae fed microparticulate diets containing the standard amount (0.08 g retinol/kg diet: group N) or all excess of retinoic acid which induces skeletal malformations (0.5 g retinol/kg diet: group RA). Real-time reverse transcriptor polymerase chain...
Tipo: Text
Palavras-chave: European sea bass; Retinoic acid; Morphogenesis; Gene expression; Endothelin 1.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6172.pdf

Nutritional components affecting skeletal development in fish larvae
Provedor de dados: 5
Autores: Cahu, Chantal; Zambonino, Jose-luis; Takeuchi, Toshio.
Marine fish larvae undergo major functional and morphological changes during the developmental stages and several factors can interfere with the normal development of larvae and affect fry quality. Skeletal malformations, such as spinal malformation-scoliosis, lordosis, coiled vertebral column-, missing or additional fin rays, bending opercle or jaw malformations, are frequently observed in hatchery-reared larvae. This paper reviews the effects of some nutritional components on skeletal development in larvae of a number of fish species. In the dietary lipid fraction, for instance, it was proven that the phospholipid concentration affected the spinal malformation rate in sea bass fed a compound diet from mouth opening onwards. Phosphatidylinositol, in...
Tipo: Text
Palavras-chave: Retinoic acid; Ascorbic acid; Amino acid; Phospholipid; Peptides; Skeletal development; Quality; Fish larvae.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-443.pdf

Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet
Provedor de dados: 5
Autores: Geay, Florian; Culi, E. Santigosa I.; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y.; Corcos, L.; Bodin, Nathalie; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol...
Tipo: Text
Palavras-chave: Sea bass (Dicentrarchus labrax); FADS2 expression and activity; HUFA biosynthesis.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00008/11929/8635.pdf

Comparison of dietary phospholipids and neutral lipids on skeletal development and fatty acid composition in Atlantic cod (Gadus morhua)
Provedor de dados: 5
Autores: Kjorsvik, Elin; Olsen, Cecilia; Wold, Per-arvid; Hoehne-reitan, Katja; Cahu, Chantal; Rainuzzo, Jose; Olsen, Atle Ivar; Oie, Gunvor; Olsen, Yngvar.
Dietary essential fatty acids may affect larval skeletal formation. The aim of this study was to compare effects on growth and osteological development of dietary docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) being incorporated in the phospholipid (diets PL1 and PL3) or in the neutral lipid (diet NL1) fraction of the larval diet for Atlantic cod (Gadus morhua). The diets were labelled according to the estimated percentage of total n-3 fatty acids contained in the dietary neutral lipid (NL1-1.3% of dietary dry matter) or in the phospholipid fraction (PL1-1.1% and PL3-2.3%). Larvae were weaned to the isoenergetic and isolipiclic microdiets from 17 days post hatching (dph). They were co-fed enriched rotifers until 24 dph and received thereafter...
Tipo: Text
Palavras-chave: DHA; EPA; Fatty acids; Phospholipids; Dietary requirements; Skeletal deformities; Osteological development; Fish larvae; Cod; Gadus morhua.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7417.pdf

Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids
Provedor de dados: 5
Autores: Hamza, Neila; Silvestre, Frederic; Mhetli, Mohamed; Ben Khemis, Ines; Dieu, Marc; Raes, Martine; Cahu, Chantal; Kestemont, Patrick.
A comparative proteomic approach was used to assess the protein expression profile in the liver of 34 days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9.5% (PL9). Using 2D-DIGE minimal labelling of liver extracts, we were able to show 56 protein spots with a differential intensity (p&lt;0.05) depending on the dietary PL content. Among these spots, 11 proteins were un-ambiguously identified using nanoLC-MS/MS tandem mass spectrometry. In the PL9 larvae, our results indicate that the glycolytic pathway could be down-regulated due to the under-expression of the fructose biphosphate aldolase B and the...
Tipo: Text
Palavras-chave: 2D-DIGE; Metabolism; Phospholipid; Pikeperch; Proteomics.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00007/11837/8575.pdf

Nutrition, digestion et développement des larves de crevettes et poissons marins
Provedor de dados: 5
Autores: Cahu, Chantal.
This report is presented by Chantal Cahu for obtaining accreditation to supervise research. Since 1981, her research activity has addressed the study of reproducers' nutrition and the young stages of development of marine organisms belonging to two different branch lines, crustaceans and fish. This work, performed in a base-lined research body, IFREMER, is placed within a more general programme of aquaculture development. Indeed, whatever species are at issue, the development of aquaculture in France, as at the global level, is based on the production of juveniles in hatcheries. My work has therefore more precisely the goal of acquiring knowledge about the nutritional needs, mechanisms of digestion and the development of these animals in order to improve...
Tipo: Text
Palavras-chave: Skeleton development; Vitamins; Nutriment; Fish larvae; Egg quality; Reproduction; Nutrition; Shrimp larvae; Peneids.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-2086.pdf

FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Tipo: Text
Ano: 2010

Dietary vitamin D-3 affects digestive system ontogenesis and ossification in European sea bass (Dicentrachus labrax, Linnaeus, 1758)
Provedor de dados: 5
Autores: Darias, Maria J.; Mazurais, David; Koumoundouros, Giorgos; Glynatsi, Nomiki; Christodoulopoulou, Stavroula; Huelvan, Christine; Desbruyeres, Elisabeth; Le Gall, Marie-madeleine; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, Jose L.
The influence of dietary vitamin D-3 (VD3) on survival, growth and morphogenesis during the larval development of European sea bass (Dicentrarchus labrax) was evaluated until 45 days post hatching. Diets contained 4% of the standard vitamin mix (VM) recommended by the National Research Council (NRC) and incorporated 0, 19.2, 38.4, or 140 IU of VD3 per gram of diet to give VD-0, VD-1, VD-2 and VD-3 dietary treatments, respectively. The present study revealed for the first time an impact of dietary VD3 on the sea bass digestive system ontogenesis that consequently conditioned the ossification process and morphogenesis. All dietary VD3 levels were in the "adequate range" based on larval survival. Nevertheless, growth, intestinal maturation and ossification at...
Tipo: Text
Palavras-chave: Fish larvae; Ossification; Malformations; Vitamin D; Digestive system; Gene expression.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00000/11147/7849.pdf

Ontogeny of the gastrointestinal tract of marine fish larvae
Provedor de dados: 5
Autores: Zambonino, Jose-luis; Cahu, Chantal.
Marine fish larvae undergo major morphological and cellular changes during the first month of life. The ontogeny of the gastrointestinal tract combines these two aspects of the larval development and is very interesting in that the timing of functional changes appears genetically hard-wired. The goal of this paper is to give an overview of the gastrointestinal development process in marine fish larvae, with particular attention to three species: sea bass; red drum; and sole, since the description of gut maturation in fish larvae was initiated during the last decade with these species. During the early stages, marine fish larvae exhibit particular digestive features. Concerning the exocrine pancreas, amylase expression decreases with age from the third week...
Tipo: Text
Palavras-chave: Proteins; Pancreatic enzymes; Lipids; Larvae feeds; Intestinal enzymes; Glucides; Fish larvae; Enzyme expression; Digestion; Development; Aquaculture.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-650.pdf

Ontogenèse des fonctions digestives et besoins nutritionnels chez les larves de poissons marins
Provedor de dados: 5
Autores: Cahu, Chantal; Zambonino, Jose-luis.
The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first...
Tipo: Text
Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf

Evaluation of microdiets versus live feeds on growth, survival and fatty acid composition of larval haddock (Melanogrammus aeglefinus)
Provedor de dados: 5
Autores: Blair, Tammy; Castell, Fisheries And Oceans Canada; Neil, Steven; D'Abramo, Louis; Cahu, Chantal; Harmon, Paul; Ogunmoye, Kehinde.
Until recently, it was considered impossible to feed newly hatched marine fish species with a compound diet. Substituting a compound diet for live prey was performed several weeks after hatching, depending on the species. Compound diets were well ingested at the early stage but larvae died with a gut full of food, suggesting that larvae were unable to digest the compound diet. The hypothesis was that younger larvae have insufficient digestive enzymes to thrive on compound diets, and that exogenous enzymes, provided from live prey, are necessary for early stages.  The organogenesis of marine fish larvae is not completely achieved at hatching and histological studies have revealed that the anatomy of the digestive tract undergoes developmental changes over...
Tipo: Text
Palavras-chave: Highly unsaturated fatty acids; Essential fatty acids; Survival; Growth; Artemia; Brachionus plicatilis; Microdiet; Haddock larvae.
Ano: 2003
URL: https://archimer.ifremer.fr/doc/2003/publication-445.pdf

Ontogeny of the digestive tract of thick lipped grey mullet (Chelon labrosus) larvae reared in "mesocosms"
Provedor de dados: 5
Autores: Zouiten, Dora; Ben Khemis, I; Besbes, R; Cahu, Chantal.
This work describes the ontogeny of the digestive tract in thick lipped grey mullet (Chelon labrosus) larvae reared until day 36 post-hatching with the semi-extensive technology in mesocosms. Diet was constituted by live preys, rotifers, Arternia and wild zooplankton, then compound diet was added from day 20 (p. h.). Linear growth, weight growth and digestive enzymes specific activities were studied during larval ontogeny. Pancreatic enzymes (trypsin and amylase) and intestinal enzymes (leucine-alanine peptidase "Leu-ala", aminopeptidase N "AN" and alkaline phosphatase "AP") were assayed in larvae sampled throughout the rearing trial to evaluate gastrointestinal maturation along the development.   The trypsin specific activities were very high during the...
Tipo: Text
Palavras-chave: Pancreatic enzymes; Intestinal enzymes; Fish larvae; Mesocosm; Chelan labrosus.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4585.pdf

Optimal levels of dietary vitamin A for reduced deformity incidence during development of European sea bass larvae (Dicentrarchus labrax) depend on malformation type
Provedor de dados: 5
Autores: Mazurais, David; Glynatsi, Nomiki; Darias, Maria J.; Christodoulopoulou, Stavroula; Cahu, Chantal; Zambonino, Jose-luis; Koumoundouros, Giorgos.
The purpose of this study was to examine the impact of graded levels of dietary vitamin A on sea bass larval performances and to determine optimal retinol levels at different larval stages to avoid specific skeletal malformations. Retinol was incorporated into larval feeds at 0, 5,10,15, 25, 35 and 70 mg kg(-1) dry matter (giving rise to RET0, RET5, RET10, RET15, RET25, RET35, RET70 groups, respectively). Analysis of the several types of deformities affecting the skull, vertebral column or fins of the fish were observed depending on experimental groups. On one hand, the incidence of skull malformations affecting the maxillary and premaxillary bones. dentaries, operculum, branchiostegal rays and glossohyal was lower for the RET0 and RET5 groups. On the...
Tipo: Text
Palavras-chave: Hoxd 9; Vitamin A; Skeletal development; Sea bass larvae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6779.pdf

Gene expression pattern of digestive and antioxidant enzymes during the larval development of reared Atlantic bluefin tuna (ABFT), Thunnus thynnus L.
Provedor de dados: 5
Autores: Mazurais, David; Coves, Denis; Papandroulakis, Nikos; Ortega, Aurelio; Desbruyeres, Elisabeth; Huelvan, Christine; Le Gall, Marie-madeleine; De La Gandara, Fernando; Cahu, Chantal.
The aim of this study was to determine whether mortality observed during the larval development of reared bluefin tuna (Thunnus thynnus) could be related to improper expression profiles of key genes involved in digestive or antioxidant response capabilities. Tuna larvae were sampled at hatching, 2, 5, 10, 15 and 20 dph (days post hatching) for the relative quantification of transcripts encoded by genes involved in digestive [trypsinogen 1 (TRYP1), alpha-amylase (AMY), aminopeptidase N (ANPEP)] and antioxidant [catalase (CAT)] functions. The levels of expression of ANPEP related to the development and maturation of intestinal function increased from 5 to 20 dph. Furthermore, AMY and TRYP1 genes, which are pancreatic enzymes implicated in carbohydrate and...
Tipo: Text
Palavras-chave: Tuna; Gene; Expression; Development; Digestion; Antioxidant.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00171/28264/26521.pdf

Gene expression patterns during the larval development of European sea bass (Dicentrarchus labrax) by microarray analysis
Provedor de dados: 5
Autores: Darias, Maria J.; Zambonino, Jose-luis; Hugot, K; Cahu, Chantal; Mazurais, David.
During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were...
Tipo: Text
Palavras-chave: Sea bass; Gene expression; Microarray; Development; Fish larvae.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4344.pdf

Effet du ratio lipides/protéines de l'aliment sur la croissance de l'ombrine ocellée (Sciaenops ocellatus) et sur les rejets biologiques
Provedor de dados: 5
Autores: Faure, Leslie; Regina, Françoise; Dutto, Gilbert; Falguiere, Jean-claude; Coves, Denis; Cahu, Chantal.
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00098/20947/18566.pdf

Comparison of dietary phospholipids and neutral lipids: effects on gut, liver and pancreas histology in Atlantic cod (Gadus morha L.) larvae
Provedor de dados: 5
Autores: Wold, P. -a.; Hoehne-reitan, K.; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J.; Kjorsvik, E.
The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly...
Tipo: Text
Palavras-chave: Stereology; Phospholipids; Nutrition; Lipid class; Larvae; Histology; Diet; Cod.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6777.pdf

Effect of dietary phospholipid levels on performance, enzyme activities and fatty acid composition of pikeperch (Sander lucioperca) larvae
Provedor de dados: 5
Autores: Hamza, N; Mhetli, M; Ben Khemis, I; Cahu, Chantal; Kestemont, P.
This study was carried out to evaluate the effects of dietary phospholipid on the development and rearing performance of pikeperch (Sander lucioperca) larvae. From day 10 post-hatching, fish larvae were weaned onto three isoproteic and isolipidic formulated diets with different phospholipid (PL) levels: 1.4 (PL1), 4.7 (PL5) and 9.5% (PL9) of dry matter, as soybean lecithin. Neutral lipid (NL) with inversed gradient was incorporated in diets. Survival, growth and deformities were monitored until day 34 post-hatching, as well as intestinal enzyme activities, leucine alanine peptidase (leu-ala), aminopeptidase N (AN) and alkaline phosphatase (AP), which were used as indicators of digestive tract maturation. This study showed that PL supplementation...
Tipo: Text
Palavras-chave: Pikeperch; Phospholipids; Larval development; Fatty acid; Digestive enzymes.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4149.pdf

L’ aquaculture : quelle réponse à la demande croissante de nourriture dans le monde ?
Provedor de dados: 5
Autores: Cahu, Chantal.
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59893/63073.pdf

Dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in European sea bass Dicentrarchus labrax larval development
Provedor de dados: 5
Autores: Gisbert, Enric; Villeneuve, Laure; Zambonino, Jose-luis; Quazuguel, Patrick; Cahu, Chantal.
We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets with different levels of EPA and DHA. Diet names indicated the percentage of EPA and DHA contained in the phospholipids (PL) and neutral lipids (NL), as follows: PL5, PL3, PL1, NL1, and NL3. Histological observations showed different patterns of lipid absorption and accumulation in the intestinal mucosa depending on the level and nature of the dietary lipid fraction. Fish fed high...
Tipo: Text
Palavras-chave: Histology; Digestive system; Phospholipid; Larvae; Dicentrarchus labrax; Dietary.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-929.pdf

Effect of nature of dietary lipids on European sea bass morphogenesis: implication of retinoid receptors
Provedor de dados: 5
Autores: Villeneuve, Laure; Gisbert, Enric; Zambonino, Jose-luis; Quazuguel, Patrick; Cahu, Chantal.
The effect of the nature and form of supply of dietary lipids on larval development was investigated in European sea bass larvae, by considering the expression of several genes involved in morphogenesis. Fish were fed from 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets incorporating different levels of EPA and DHA provided by phospholipid or neutral lipid. Phospholipid fraction containing 1 center dot 1 % (PL1 diet) to 2 center dot 3 % (PL3 diet) of EPA and DHA sustained good larval growth and survival, with low vertebral and cephalic deformities. Similar levels of EPA and DHA provided by the neutral lipid fraction were teratogenic and lethal. Nevertheless, dietary phospholipids containing high levels of DHA and EPA (PL5 diet)...
Tipo: Text
Palavras-chave: Retinoic acid receptors; Skeletal malformations; Dicentrarchus labrax larvae; Neutral lipids; Phospholipids.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-880.pdf

Effect of nutrition on marine fish development and quality
Provedor de dados: 5
Autores: Zambonino, Jose-luis; Cahu, Chantal.
Fish larval stage represents a transitional period in which both ontogeny and growth cause substantial changes in structure, physiology, size and morphology. These last twenty years, most of the studies on marine fish larvae development have dealt with the ontogeny and functioning of the digestive tract in order to overcome bottlenecks in larvae culture and weaning processes (switch from live preys to compound diet feeding sequence). These studies clearly demonstrated that fish larvae are not faced to physiological or digestive deficiencies (Zambonino Infante and Cahu, 2001) although they hatch with very immature organs compared to juveniles.  More recently, the possible impact of nutritional events occurred during early larvae development stages were...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00086/19683/17306.pdf

Transcriptomics for understanding marine fish larval development
Provedor de dados: 5
Autores: Mazurais, David; Darias, M.; Zambonino, Jose-luis; Cahu, Chantal.
The larval phase is a crucial period in the life of marine fish. During this phase, the organism will acquire the phenotype of an adult fish through the development of tissues and organs and the maturation of some of the principal physiological functions. Many biological processes (differentiation, cellular proliferation, growth, etc.) are regulated during this period. These regulations take place at different biological levels and particularly concern the expression of genes involved in larval ontogenesis processes. The development of bioinformatic resources (DNA or cDNA sequences) and molecular tools enabling high throughput gene expression analysis (microarrays) have allowed the transcriptome of marine fish species to be studied. In the present review,...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00044/15488/12962.pdf

Biofloc technology applied to rear shrimp Litopenaeus Stylirostris broodstock: An integrated and development research project in New Caledonia and French Polynesia
Provedor de dados: 5
Autores: Chim, Liet; Cardona, Emilie; Lorgeoux, Benedicte; Gueguen, Yannick; Saulnier, Denis; Goguenheim, Jean; Wantiez, Laurent; Cahu, Chantal.
Shrimp farming in New-Caledonia faces up to difficulties. A fluctuating quality of broodstock prevents sustainable production of larvae in quantity and quality. The traditional extensive rearing method of broodstock in outdoor earthen ponds used in New Caledonia raises several issues: poor water quality control and biosecurity. Therefore it is fundamental to modify the broodstock culture strategy in order to achieve an easier water quality management and maximal biosecurity. Biofloc technology (BFT) offers easier water quality management, higher natural productivity, higher level of biosecurity, and could be a good alternative. Since 2011 a R&amp;D program started under the supervision of IFREMER in New Caledonia and French Polynesia in order to study and...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00250/36084/34635.pdf

An early-life hypoxia event has a long-term impact on protein digestion and growth in European sea bass juvenile.
Provedor de dados: 5
Autores: Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy.
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes.  Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group.  We examined...
Tipo: Text
Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf

Ontogenèse, Développement et Physiologie chez les larves de poissons
Provedor de dados: 5
Autores: Gatesoupe, Joel; Zambonino-infante, Jose-luis; Cahu, Chantal; Bergot, P.
Chez les poissons, le terme de larve désigne les plus jeunes individus entre le moment où ils sont capables d'ingérer de la nourriture exogène et celui où ils prennent la forme de l'adulte. Le stade larvaire suit le stade embryonnaire et précède le stade juvénile. II correspond à une période de transformation morphologique et physiologique continue de l'animal. La larve partage avec l'embryon certaines caractéristiques comme une très petite taille, un taux de croissance spécifique très élevé et une différenciation incomplète de certains organes. Cependant, elle est déjà capable de capturer et digérer des proies.
Tipo: Text
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00086/19713/17354.pdf