Consulta: creatorFacets:"Caimi,Karina C."
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LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
Provedor de dados: Brazilian Journal of Microbiology
Autores: Trangoni,Marcos D.; Gioffré,Andrea K.; Cerón Cucchi,María E.; Caimi,Karina C.; Ruybal,Paula; Zumárraga,Martín J.; Cravero,Silvio L.
In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loop-mediated isothermal amplification; Molecular typing; Brucellosis; Paratuberculosis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619