Consulta: creatorFacets:"Campos-Takaki,Galba Maria de"
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Data/hora: 04/06/2024 04:48:57

Growth of Cunninghamella elegans UCP 542 and production of chitin and chitosan using yam bean medium
Provedor de dados: 69
Autores: Stamford,Thayza Christina Montenegro; Stamford,Tânia Lucia Montenegro; Stamford,Newton Pereira; Barros Neto,Benicio de; Campos-Takaki,Galba Maria de.
Microbiological processes were used for chitin and chitosan productions by Cunninghamella elegans (UCP 542) grown in a new economic culture medium. The assay was carried out to evaluate the growth of C. elegans using yam bean (Pachyrhizus erosus L. Urban) medium, in different times of growth (24, 48, 72 and 96 hrs), incubated at 28ºC in an orbital shaker at 150 rpm. The lyophilized biomass was determined by gravimetry. The polysaccharides were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, titration and viscosity. C. elegans grown in the yam bean medium and produced higher yields of biomass (24.3 g/ mL) in 96 hrs. The high level was chitosan (66 mg/g), and chitin (440 mg/g) were produced at 48 and 72 hrs of growth,...
Tipo: Journal article
Palavras-chave: Biopolymers; Chitin; Chitosan.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100006

A new biosurfactant produced by Candida glabrata UCP 1002: characteristics of stability and application in oil recovery
Provedor de dados: 52
Autores: Luna,Juliana Moura de; Sarubbo,Leonie; Campos-Takaki,Galba Maria de.
The production of a new biosurfactant by Candida glabrata UCP1002 was studied to evaluate the influence of the concentration of the cotton seed oil, glucose and yeast extract. The dynamics of the growth and surfactant production were showed for all the cultivation conditions studied. The best emulsification of the n-hexadecane, quantified by the emulsifying index was observed in the medium containing 7.5% cotton seed oil, 5% glucose and 0.3% yeast extract. The isolated biosurfactant showed a CMC of 2.5% and the surface tension at that point showed to be 31mN/m. The potential application of the biosurfactant in oil recovery from the sand, in acid and alkaline environments and over exposure to high salinity and different temperatures was demonstrated by the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosurfactant; Candida glabrata; Surface tension; Stability; Cotton seed oil; Oil removal.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400001

Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)
Provedor de dados: 52
Autores: Sarubbo,Leonie Asfora; Oliveira,Luciana Alves de; Porto,Ana Lúcia Figueiredo; Campos-Takaki,Galba Maria de; Tambourgi,Elias Basile.
The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aqueous two-phase systems; Ine serum albumin; Cashew-nut tree gum; Protein partition.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500003

New bioemulsifiers produced by Candida lipolytica using D-glucose and babassu oil as carbon sources
Provedor de dados: 58
Autores: Vance-Harrop,Mabel H.; Gusmão,Norma B. de; Campos-Takaki,Galba Maria de.
Candida lipolytica IA 1055 produced extracellular biosurfactants with emulsification activity by fermentation using babassu oil and D-glucose as carbon sources. Natural seawater diluted at 50% supplemented with urea, ammonium sulfate, and phosphate was used as economic basal medium. The best results were achieved with the YSW-B2 medium, which contained urea, ammonium sulfate, and babassu oil and with YSW-B3 medium, which contained urea, ammonium sulfate, phosphate, and babassu oil, kept under fed batch fermentation for 60 hours with 5% of babassu oil. For the two media, the maximum specific growth rates were 0.02 h-1 and 0.04 h-1; the generation times were 34.6 h-1 and 17.3 h-1, and the emulsification activities were 0.666 and 0.158 units, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioemulsifier; Candida lipolytica; Seawater; Babassu oil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200006

Faster chitosan production by mucoralean strains in submerged culture
Provedor de dados: 58
Autores: Amorim,Rosa Valéria da Silva; Souza,Wanderley de; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de.
Production of chitosan was conducted using two Mucoralean strains, Mucor racemosus and Cunninghamella elegans. Chitosan was extracted from mycelia of M. racemosus and C. elegans at different growth phases on YPD medium. In both fungi, chitosan was rapidly produced, while highest yield of extractable chitosan was found in 24h of cultivation in submerged culture. The yield of chitosan isolated from dry mycelia of M. racemosus was about 40% higher than from C. elegans. The degree of N-acetylation of chitosan was 49% in M. racemosus and 20% in C. elegans, and the D-glucosamine contents were about 48% and 90%, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitosan; Submerged culture; Mucor racemosus; Cunninghamella elegans.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100005

Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production
Provedor de dados: 58
Autores: Carneiro-da-Cunha,Maria das Graças; Lima Filho,José Luiz de; Campos-Takaki,Galba Maria de.
Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/mL), in an osmotic solution for 90 min at 28ºC. The frequency of regenerated protoplasts in the lag phase was 1.7x10³ CFU/mL (28.97%), in the beginning of the exponential phase 0.4x10² CFU/mL (31.67%), in the exponential growth phase 2.5x10³ CFU/mL (46.30%) and 1.0x10(5) CFU/mL in stationary phase (48.45%). Antibiotic production and activity of regenerated protoplasts were observed in all phases, except in the lag phase. The protoplast formation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomyces clavuligerus; Protoplast; Clavulanic acid.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400014

Production of extracellular proteases by Mucor circinelloides using D-glucose as carbon source / substrate
Provedor de dados: 58
Autores: Andrade,Vânia Sousa; Sarubbo,Leonie Asfora; Fukushima,Kasutaka; Miyaji,Makoto; Nishimura,Kazuko; Campos-Takaki,Galba Maria de.
Recently, some Mucorales species have been reported as protease producers. The production of extracellular proteases by Mucor circinelloides using glucose as substrate was studied. Experiments were carried out with different D-glucose concentrations (40, 60 and 80 g/L). Biomass, pH and protease activity were determined. Although biomass production had reached best yields for the medium containing D-glucose in a concentration of 80 g/L, the enzymatic production was higher when the substrate concentration was reduced to 40 g/L. The yield factor for product on cell growth and the yield factor for product on carbon substrate were higher when the microorganism grew in medium containing 40 g/L glucose. The kinetics parameters suggest that this strain seems to be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular proteases; D-glucose; Substrate; Mucor circinelloides.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200002

Heavy metal biosorption by chitin and chitosan isolated from Cunninghamella elegans (IFM 46109)
Provedor de dados: 58
Autores: Franco,Luciana de Oliveira; Maia,Rita de Cássia C.; Porto,Ana Lúcia F.; Messias,Arminda Sacconi; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de.
Chitin and chitosan were extracted from mycelial biomass of Cunninghamella elegans and the performance for copper, lead and iron biosorption in aqueous solution was evaluated. The growth curve of C. elegans was accomplished by determination of biomass, pH, glucose and nitrogen consumption. Chitin and chitosan were extracted by alkali-acid treatment and the yields were 23.8 and 7.8%, respectively. For the adsorption analysis, the process of heavy uptake metal sorption was evaluated using polysaccharides solutions (1% w/v). The rate of metallic biosorption was dependent upon the concentration and pH of metal solutions, and the best results were observed with pH 4.0. Chitosan showed the highest affinity for copper and chitin for iron adsorption. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cunninghamella elegans; Chitin; Chitosan; Bioremediation; Biosorption.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200013

Polyphosphate in Zygomycetes: a cytochemical study
Provedor de dados: 58
Autores: Shari'a,Allana Elesbão do Nascimento; Nascimento,Aline Elesbão do; Lima,Marcos Antonio Barbosa de; Campos-Takaki,Galba Maria de; Souza,Wanderley de.
The inorganic phosphorus content, and distribution, structure and localization of polyphosphate in mycelia of zygomycetous fungi was evaluated. Ultrastructural cytochemistry was successfully used to identifying the localization and distribution of polyphosphate in Absidia cylindrospora, Gongronella butleri and Mucor javanicus. The results revealed differences in the cytochemical staining pattern in all species studied and a uniform labeling on the cellular surface (cell wall and citoplasmic membrane). Reaction products were observed in intracellular structures and cellular membrane. Intracellular staining was observed in trabecullar, vacuolar and vesicular structures, in dense bodies and in the cytoplasm. However, the cytochemical staining intensity varied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrastructural cytochemistry; Polyphosphate; Zygomycetes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200004

Effects of phosphorus on polyphosphate accumulation by Cunninghamella elegans
Provedor de dados: 58
Autores: Lima,Marcos Antonio Barbosa de; Nascimento,Aline Elesbão do; Souza,Wanderley de; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de.
The content of inorganic polyphosphate and the polymeric degree of these compounds were evaluated during the growth of Cunninghamella elegans in medium containing varying orthophosphate (Pi) concentrations. For this purpose, a combination of chemical methods for polyphosphate extraction and ultrastructural cytochemistry were used. The orthophosphate and glucose consumption was also determined during the fungal cultivation. At Pi concentrations of 0.5, 2.5 and 0.0 g/L, the maximum amounts of biomass were 3.18, 3.29 and 0.24 g/L, respectively. During growth the cells accumulated Pi from the medium. At three days of growth the biomass consumed up to 100 and 95% of Pi from the media at initial concentrations of 0.5 and 2.5 g/L, respectively. Polyphosphate was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyphosphate; Accumulation; Ultrastructural cytochemistry; Cunninghamella elegans; Ortophosfate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400016