Consulta: creatorFacets:"Carnieli Jr.,Pedro"
Registros recuperados: 2
Data/hora: 10/06/2024 06:46:44

Short-interfering RNAs as antivirals against rabies
Provedor de dados: 55
Autores: Brandão,Paulo Eduardo; Castilho,Juliana Galera; Fahl,Willian; Carnieli Jr.,Pedro; Oliveira,Rafael de Novaes; Macedo,Carla Isabel; Carrieri,Maria Luiza; Kotait,Ivanete.
This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SiRNA; Rabies; Antiviral.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011

In vitro and in vivo inhibition of rabies virus replication by RNA interference
Provedor de dados: 58
Autores: Ono,Ekaterina A. Durymanova; Iamamoto,Keila; Castilho,Juliana G.; Carnieli Jr.,Pedro; Oliveira,Rafael de Novaes; Achkar,Samira M.; Carrieri,Maria L.; Kotait,Ivanete; Brandão,Paulo E.
Rabies is a zoonotic disease that affects all mammals and leads to more than 55,000 human deaths every year, caused by rabies virus (RABV) (Mononegavirales: Rhabdoviridae: Lyssavirus). Currently, human rabies treatment is based on the Milwaukee Protocol which consists on the induction of coma and massive antiviral therapy. The aim of this study was to assess the decrease in the titer of rabies virus both in vitro and in vivo using short-interfering RNAs. To this end, three siRNAs were used with antisense strands complementary to rabies virus nucleoprotein (N) mRNA. BHK-21 cells monolayers were infected with 1000 to 0.1 TCID50 of PV and after 2 hours the cells were transfected with each of tree RNAs in separate using Lipofectamine-2000. All three siRNAs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; RNA interference; SiRNAs; Antiviral; Treatment.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300034