Consulta: creatorFacets:"Cestari,Marta Margarete"
Registros recuperados: 11
Data/hora: 05/06/2024 18:16:41

Karyotypic characterization of Hydromedusa tectifera (Testudines, Pleurodira) from the upper Iguaçu River in the Brazilian state of Paraná
Provedor de dados: 74
Autores: Noleto,Rafael Bueno; Kantek,Daniel Luis Zanella; Swarça,Ana Cláudia; Dias,Ana Lúcia; Fenocchio,Alberto Sergio; Cestari,Marta Margarete.
We present the karyotypic characterization of 26 specimens of the side-necked turtle Hydromedusa tectifera collected in the upper Iguaçu River, Paraná state, Brazil. The turtles were cytogenetically analyzed using Giemsa staining and other banding techniques (C, G, Ag-NOR and CMA3) as well as fluorescence in situ hybridization (FISH) with a rDNA 18S probe. All the specimens showed a diploid number of 58 composed of 22 macro and 36 microchromosomes. The Ag-NOR, CMA3 and FISH techniques permitted the identification and characterization of the chromosome pairs bearing nucleolus organizer regions (NORs), while G-banding facilitated a better recognition and pairing of macrochromosomes. These data agree with some information available in the literature and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomes; FISH; G-banding; Pleurodira; Turtle.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200011

Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests
Provedor de dados: 74
Autores: Ferraro,Marcos Vinícius M.; Fenocchio,Alberto Sérgio; Mantovani,Mario Sérgio; Ribeiro,Ciro de Oliveira; Cestari,Marta Margarete.
Genotoxicity studies on toxic metals and their organic compounds are very important, especially so in the investigation of the effects of these compounds on the aquatic environments where they tend to accumulate. The use of endemic aquatic organisms as biological sentinels has proved useful to environmental monitoring. We assessed the mutagenic potential of tributyltin (TBT) and inorganic lead (PbII) using samples of the fish Hoplias malabaricus (commonly called traíra) using the comet assay and the piscine micronucleus and chromosome aberration tests. Eighteen H. malabaricus were acclimatized in three individual aquariums, each containing six fish, six fish being exposed to 0.3 mg/g of body weight (bw) of TBT, six to 21 mg/g bw of PbII and six being used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus; Chromosome aberrations; Tributyltin; Lead; Fish.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100017

Population analysis of a chromosome polymorphism in Astyanax (Teleostei, Characiformes) species endemic to the Iguaçu River
Provedor de dados: 74
Autores: Kantek,Daniel Luis Zanella; Cipriano,Roger Raupp; Noleto,Rafael Bueno; Fenocchio,Alberto Sergio; Artoni,Roberto Ferreira; Cestari,Marta Margarete.
Chromosomal analyses were performed in the fish Astyanax sp.D collected from three different points: two streams from the right bank and one from the left bank of the Upper Iguaçu River, Paraná State, Brazil. The individuals from all localities possess 2n = 50 chromosomes and a FN = 84 (4m+24sm+6st+16a). The C-banding pattern was similar in all populations. However, within each population, an interindividual variation concerning the number and localization of heterochromatic bands was observed. Some of these variations were quantified in each population, and the results indicate that the samples were not different when studying the variable frequencies. Considering that Astyanax sp.D is typical in the headwaters of the Iguaçu River, these results were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Population cytogenetics; Chromosome variations; Astyanax fish.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200013

Genotoxicity of titanium dioxide nanoparticles and triggering of defense mechanisms in Allium cepa
Provedor de dados: 74
Autores: Santos Filho,Ronaldo dos; Vicari,Taynah; Santos,Samuel A.; Felisbino,Karoline; Mattoso,Ney; Sant’Anna-Santos,Bruno Francisco; Cestari,Marta Margarete; Leme,Daniela Morais.
Abstract Titanium dioxide nanoparticles (TiO2NPs) are widely used and may impact the environment. Thus, this study used a high concentration of TiO2NP (1000 mg/L) to verify the defense mechanisms triggered by a plant system – an indicator of toxicity. Furthermore, this study aimed at completely characterizing TiO2NP suspensions to elucidate their toxic behavior. TiO2NPs were taken up by meristematic cells of Allium cepa, leading to slight inhibition of seed germination and root growth. However, severe cellular and DNA damages were observed in a concentration-dependent manner (10, 100, and 1000 mg/L). For this reason, we used the highest tested concentration (1000 mg/L) to verify if the plant cells developed defense mechanisms against the TiO2NPs and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Micronuclei; Nucleolar alterations; Cellular alterations; Nanoparticle phase-change.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300425

DNA damage in the kidney tissue cells of the fish Rhamdia quelen after trophic contamination with aluminum sulfate
Provedor de dados: 74
Autores: Klingelfus,Tatiane; Costa,Paula Moiana da; Scherer,Marcos; Cestari,Marta Margarete.
Abstract Even though aluminum is the third most common element present in the earth's crust, information regarding its toxicity remains scarce. It is known that in certain cases, aluminum is neurotoxic, but its effect in other tissues is unknown. The aim of this work was to analyze the genotoxic potential of aluminum sulfate in kidney tissue of the fish Rhamdia quelen after trophic contamination for 60 days. Sixty four fish were subdivided into the following groups: negative control, 5 mg, 50 mg and 500 mg of aluminum sulfate per kg of fish. Samples of the posterior kidney were taken and prepared to obtain mitotic metaphase, as well as the comet assay. The three types of chromosomal abnormalities (CA) found were categorized as chromatid breaks,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Metal contamination; Chromosomal abnormalities; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400499

Cytogenetics and DNA barcode reveal an undescribed Apareiodon species (Characiformes: Parodontidae)
Provedor de dados: 74
Autores: Santos,Emanoel Oliveira dos; Deon,Geize Aparecida; Almeida,Rafael Bonfim de; Oliveira,Ezequiel Aguiar de; Nogaroto,Viviane; Silva,Hugmar Pains da; Pavanelli,Carla Simone; Cestari,Marta Margarete; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo.
Abstract Parodontidae is a small group of fish and some species are particularly difficult to identify due to the lack of sufficiently consistent morphological traits. Cytogenetically, the species possess 2n = 54 chromosomes and are either sex-homomorphic or sex-heteromorphic (regarding its chromosomes). We evaluated data on color, tooth morphology, cytogenetics, and mitochondrial markers (COI) in Apareiodon specimens from the Aripuanã River (Amazon basin) and the results were compared to other congeneric taxa. Morphological results show an overlap of body color and tooth morphology to other known Apareiodon. The cytogenetics data showed that the 2n = 54 chromosomes, 50 m/sm + 4 st and, a ZZ/ZW sex chromosome system in Apareiodon sp. are common to other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal differentiation; Hidden diversity; Repetitive DNA; Sex chromosomes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300365

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations
Provedor de dados: 74
Autores: Cestari,Marta Margarete; Lemos,Priscilla Maria M.; Ribeiro,Ciro Alberto de Oliveira; Costa,João Ricardo M. Alves; Pelletier,Emilien; Ferraro,Marcos V.M.; Mantovani,Mário Sérgio; Fenocchio,Alberto Sergio.
The effects of clastogenic or mutagenic agents have rarely been studied in neotropical fish species exposed to contaminated water. In this study, the genetic damage caused by lead in the widely distributed South American fish, Hoplias malabaricus, was assessed using the comet (SCGE) assay and by testing for chromosomal aberrations. Eighteen specimens were acclimatized to laboratory conditions and then chronically exposed to contaminated food by feeding prey (Cyprinus sp.) injected intraperitoneally with doses of inorganic lead adjusted to give a contamination level of 21 mg of Pb2+.g-1 net weight of H. malabaricus. Three fish were sampled for chromosomal analysis after four doses (18 days) and another three after eight doses (41 days) of lead and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Comet assay; Genotoxicity; Hoplias malabaricus; Lead; Mutagenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200023

Genotoxic effect of Phenanthrene on Chironomus sancticaroli (Diptera: Chironomidae)
Provedor de dados: 103
Autores: Morais,Gisele dos Santos; Pesenti,Emanuele Cristina; Cestari,Marta Margarete; Navarro-Silva,Mário Antônio.
Phenanthrene, a Polycyclic Aromatic Hydrocarbon, remains adsorbed to sedimentary particles in aquatic environments. It affects mainly benthic organisms, and is considered potentially genotoxic. In ecotoxicology, species of Chironomus Meigen, 1803 are widely known as bioindicators of the effects of chemicals on aquatic organisms. This study investigates the effects of phenanthrene on the size of the head capsule of Chironomus sancticaroli Strixino &amp;amp; Strixino, 1981 larvae after chronic (eight days) exposure, and DNA damage after acute (96 hours) and chronic exposure (eight days), under laboratory conditions. DNA damage, evaluated using the alkaline comet assay, detected effects for both exposure periods, indicating that phenanthrene is toxic for C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioassay; Bioindicador; Development; DNA damage; PHAs.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702014000400003

Chromosomal studies of five species of the marine fishes from the Paranaguá Bay and the karyotypic diversity in the marine teleostei of the Brazilian coast
Provedor de dados: 52
Autores: Cipriano,Roger Raupp; Fenocchio,Alberto Sérgio; Artoni,Roberto Ferreira; Molina,Wagner; Noleto,Rafael Bueno; Kantek,Daniel Luis Zanella; Cestari,Marta Margarete.
In this study, five species of marine fishes from the Paranaguá Bay in the Brazilian coast were evaluated. Eucinostomus argenteus and Diapterus rhombeus (Gerreidae) presented 48 chromosomes, all of which more acrocentric (FN = 48); Strongylura timucu and S. marina (Belonidae) also presented 48 chromosomes, but with a higher karyotypic complexity than the Gerreidae, 10M+2SM+36A (FN = 60) and 4M+44A (FN = 52), respectively. The fifth species, Mugil curema (Mugilidae), different than the others, presented only 28 chromosomes 20M+4ST+4A (FN = 48). The species presented diversity in the karyotypic macro-structure, which should be relevant for the cytotaxonomy and the evolution of this group of the vertebrate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine fishes; Karyotype; Cytotaxonomy; Karyotypic evolution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200010

Cytotaxonomic and evolutionary considerations about karyotipic data of fishes from the Iguaçu River Basin in South of Brazil
Provedor de dados: 52
Autores: Kantek,Daniel Luis Zanella; Cipriano,Roger Raupp; Abilhoa,Vinícius; Artoni,Roberto Ferreira; Cestari,Marta Margarete.
The cytogenetic data available in the literature about the ichthyofauna of the Iguaçu River basin were analyzed in this review. The ichthyofauna was characterized by the high level of endemism and by the low diversity of species. Twenty-four of the eighty-one species were already karyotyped; six Characiformes, fourteen Siluriformes and four Perciformes. The chromosomal data showed the taxonomic and systematic complexity of the groups. Hypothesis related to the evolution of some Characiformes and Siluriformes groups from the Iguaçu River are proposed, as well as the utilization of karyotypic data for cytotaxonomy.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neotropical region; Fish cytogenetics; Chromosome formulae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500007

Cytotaxonomy, heterochromatic polymorphism and natural triploidy of a species of Astyanax (Pisces, Characidae) endemic to the Iguaçu river basin
Provedor de dados: 52
Autores: Kantek,Daniel Luis Zanella; Noleto,Rafael Bueno; Fenocchio,Alberto Sérgio; Cestari,Marta Margarete.
Cytogenetic analysis with Astyanax sp. D revealed a karyotype of 2n=50 with 2M+26SM+6ST+16A, besides a triploid specimen showing 2n=75 chromosomes (3M+39SM+9ST+24A). C-banding strongly stained the terminal regions of several SM-ST-A chromossomes. Two pairs of acrocentric chromosomes presented interstitial heterochromatin, this state being polymorphic and occuring due to possible paracentric inversions. The results obtained with the AluI restriction enzyme and A3 chromomycin were similar to the C-banding. Relationships were proposed between Astyanax sp. D and A. scabripinnis, as well as considerations for a possible origin of the triploid specimen (2n=3x=75). When comparing the present results with cytogenetic features of other endemic Astyanax species in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Triploidy; Paracentric inversion.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100008