Consulta: creatorFacets:"Chair, M."
Registros recuperados: 11
Data/hora: 14/06/2024 03:35:57

Uptake of &lt;i&gt;Vibrio anguillarum&lt;/i&gt; vaccine by &lt;i&gt;Artemia salina&lt;/i&gt; as a potential oral delivery system to fish fry
Provedor de dados: Open Marine Archive
Autores: Campbell, R.; Adams, A.; Tatner, M.F.; Chair, M.; Sorgeloos, P.
An ELISA to detect &lt;i&gt;Vibrio anguillarum&lt;/i&gt; antigen in &lt;i&gt;Artemia salina&lt;/i&gt; was developed. This assay was then used to determine the time course of antigen uptake by &lt;i&gt;Artemia&lt;/i&gt; during incubations with &lt;i&gt;Vibrio&lt;/i&gt; vaccine with a view to using &lt;i&gt;Artemia&lt;/i&gt; as a vaccine carrier for oral vaccination of fish fry. The rate of uptake of vaccine by &lt;i&gt;Artemia&lt;/i&gt; was shown to be concentration dependent. At a dose of 1.5x10&lt;sup&gt;7&lt;/sup&gt; cfu ml&lt;sup&gt;-1&lt;/sup&gt;, 1 mg wet weight of &lt;i&gt;Artemia&lt;/i&gt; took up 1x10&lt;sup&gt;5&lt;/sup&gt; cfu within 30min and reached maximum of 6x10&lt;sup&gt;6&lt;/sup&gt; cfu after 60min. Thus each &lt;i&gt;Artemia&lt;/i&gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia salina; Vibrio anguillarum.
Ano: 1993
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2668

Vaccination of European sea bass fry through bioencapsulation of &lt;i&gt;Artemia&lt;/i&gt; nauplii
Provedor de dados: Open Marine Archive
Autores: Chair, M.; Gapasin, R.S.J.; Dehasque, M.; Sorgeloos, P.
European sea bass (&lt;i&gt;Dicentrarchus labrax&lt;/i&gt;) fry vaccinated orally by bioencapsulation in &lt;i&gt;Artemia nauplii&lt;/i&gt; or by bath method exhibited better performance than control fish in terms of growth, food conversion and resistance to stress. The comparable survival between vaccinated and non-vaccinated animals suggests that vaccination methods are stressful. The present study shows that oral vaccination can be used to enhance growth in fish fry.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia.
Ano: 1994
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2554

Live-food mediated drug delivery as a tool for disease treatment in larviculture: the enrichment of therapeutics in rotifers and &lt;i&gt;Artemia&lt;/i&gt; nauplii
Provedor de dados: Open Marine Archive
Autores: Verpraet, R.; Chair, M.; Léger, P.; Nelis, H.; Sorgeloos, P.; De Leenheer, A.P.
The live food enrichment technique using emulsions for nutritional deficiencies in rotifers and &lt;i&gt;Artemia&lt;/i&gt; was further investigated as a tool for transferring therapeutics through the food chain. &lt;i&gt;Artemia&lt;/i&gt; nauplii and the rotifer &lt;i&gt;Brachionus plicatilis&lt;/i&gt; were enriched with a self-emulsifying concentrate in which the therapeutic mixture trimetoprim: sulfamethoxazole was incorporated. An analytical method and protocol for the quantification of trimethoprim and sulfamethoxazole in a heterogeneous biological matrix, such as &lt;i&gt;Artemia&lt;/i&gt; , was developed using High Performance Liquid Chromatography. When applying a 24h enrichment period, the accumulation of both drugs in &lt;i&gt;Artemia&lt;/i&gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture systems; Disease control; Drugs; Food chains; Therapy; Artemia.
Ano: 1992
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2894

Accumulation of trimethoprim, sulfamethoxazole, and &lt;i&gt;N&lt;/i&gt;-acetylsulfamethoxazole in fish and shrimp fed medicated &lt;i&gt;Artemia franciscana&lt;/i&gt;
Provedor de dados: Open Marine Archive
Autores: Chair, M.; Nelis, H.J.; Leger, P.; Sorgeloos, P.; De Leenheer, A.P.
In a previous paper (H.J. Nelis, P. Léger, P. Sorgeloos, and A.P. De Leenheer, Antimicrob. Agents Chemother. 35:2486-2489, 1991) it was reported that two selected antibacterial agents, i.e. trimethoprim and sulfamethoxazole, can be efficiently bioencapsulated in nauplii of the brine shrimp &lt;i&gt;&lt;i&gt;Artemia&lt;/i&gt; franciscana&lt;/i&gt; for administration to fish. This follow-up study showed that larvae of the sea bass and the turbot as well as postlarvae of the white shrimp accumulate the therapeutic agents in high quantities when fed medicated &lt;i&gt;A. franciscana&lt;/i&gt;. To monitor their levels as a function of time, the liquid chromatographic method originally developed for the analysis of &lt;i&gt;A. franciscana&lt;/i&gt; was modified...
Tipo: Info:eu-repo/semantics/article
Ano: 1996
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2923

Drug assimilation in the tissue of European sea bass (&lt;i&gt;Dicentrarchus labrax&lt;/i&gt;) fry delivered orally through bioencapsulation
Provedor de dados: Open Marine Archive
Autores: Gapasin, R.S.J.; Nelis, H.; Chair, M.; Sorgeloos, P.
Assimilation levels of the antibacterials trimethoprim (TMP) and sulfamethoxazole (SMX) in sea bass (&lt;i&gt;Dicentrarchus labrax&lt;/i&gt;) fry tissue administered orally were investigated. A 1:5 TMP and SMX combination incorporated in oil emulsion (Selco) at 20% and 40% concentrations (w/w) were bioencapsulated in &lt;i&gt;Artemia&lt;/i&gt; (Instar II) nauplii. Chemotherapeutics-loaded ('medicated') nauplii were fed to the sea bass fry and drug concentration in the tissue were analysed by high-performance liquid chromatography (HPLC). Fish fed 40% 'medicated' &lt;i&gt;Artemia&lt;/i&gt; assimilated significantly higher levels of chemotherapeutics compared with those fed 20% 'medicated' &lt;i&gt;Artemia&lt;/i&gt; . Chemotherapeutics given at 40% reached...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dicentrarchus labrax (Linnaeus; 1758) [Bass].
Ano: 1996
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2964

Mode of infection and spread of &lt;i&gt;Vibrio anguillarum&lt;/i&gt; in turbot &lt;i&gt;Scophthalmus maximus&lt;/i&gt; larvae after oral challenge through life feed
Provedor de dados: Open Marine Archive
Autores: Grisez, L.; Chair, M.; Sorgeloos, P.; Ollevier, F.P.
The infection route of the marine fish pathogen &lt;i&gt;Vibrio anguillarum&lt;/i&gt; was studied after oral challenge of the juvenile turbot &lt;i&gt;Scophthalmus maximus&lt;/i&gt; L. through a life feed. &lt;i&gt;Artemia&lt;/i&gt; nauplii were incubated in a suspension of &lt;i&gt;V. anguillarum&lt;/i&gt; cells, and subsequently fed twice to the fish. All challenged fish died within 4d after the first challenge, while no mortality occurred in the non-challenged controls. The results of an immunohistochemical examination of the sectioned fish samples clearly demonstrated that &lt;i&gt;V. anguillarum&lt;/i&gt; cells were ingested by the &lt;i&gt;Artemia&lt;/i&gt; and that the latter were ingested by the fish. Bacteria were released from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scophthalmus maximus (Linneaus; 1758) Vibrio anguillarum.
Ano: 1996
URL: http://www.vliz.be/imisdocs/publications/299074.pdf

Bioencapsulation of the antibacterial drug sarafloxacin in nauplii of the brine shrimp &lt;i&gt;Artemia franciscana&lt;/i&gt;
Provedor de dados: Open Marine Archive
Autores: Dixon, B.A.; Van Poucke, S.; Chair, M.; Dehasque, M.; Nelis, H.J.; Sorgeloos, P.; De Leenheer, A.P.
Nauplii of the brine shrimp &lt;i&gt;Artemia franciscana&lt;/i&gt; were enriched with the antibacterial drug sarafloxacin hydrochloride to determine if levels effective against four pathogenetic strains of &lt;i&gt;Vibrio&lt;/i&gt; spp. would accumulate in brine shrimp. Three vibrio strains were &lt;i&gt;Vibrio anguillarum&lt;/i&gt;; the fourth was &lt;i&gt;V. vulnificus&lt;/i&gt;. Concentrations of sarafloxacin ranging from 1 to 40% (weight: volume) were incorporated into the oil phase of Super Selco brine shrimp diets. Enriched nauplii and controls were sampled at 2h interval for 24h. Antibiotic sensitivity testing was evaluated with a modification of the Kirby-Bauer disk diffusion method. A microbiological assay with &lt;i&gt;Escherichia coli&lt;/i&gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia franciscana.
Ano: 1995
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2560

Live-food mediated drug delivery as a tool for disease treatment in larviculture: 2. A case study with European seabass
Provedor de dados: Open Marine Archive
Autores: Chair, M.; Romdhane, M.S.; Dehasque, M.; Nelis, H.; De Leenheer, A.P.; Sorgeloos, P.
The purpose of this study is to investigate the possibility of oral delivery of the antibiotics Trimethoprim (TMP) and Sulfamethoxazole (SMX) to marine fish larvae through incorporation of these antibiotics into the live food organism &lt;i&gt;Artemia&lt;/i&gt;. SMX and TMP were selected for their wide range action against gram negative pathogenic bacteria and because of their limited solubility in seawater. Ormetoprim-Sulfadimethoxine (brand name Romet-30) is a new combination of a similar antimicrobial drug used in aquaculture. Diseased penaeid larvae fed Romet-30 through the food chain exhibited greater survival than the untreated ones. In an earlier study Verpraet have described a technique for bioencapsulating high doses of more than 500 mu g TMP +...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotics; Aquaculture techniques; Disease control; Fish culture; Fish larvae; Food additives; Food organisms; Dicentrarchus labrax [Bass].
Ano: 1991
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2805

Live food mediated drug delivery as a tool for disease treatment in larviculture: a case study with turbot &lt;i&gt;Scophthalmus maximus&lt;/i&gt;
Provedor de dados: Open Marine Archive
Autores: Chair, M.; Dehasque, M.; Sorgeloos, P.; Nelis, H.; De Leenheer, A.P.
With the advent of intensive culture practices, occurrences of mass mortality caused by bacterial infections are commonplace. High doses of antibiotics or antibacterials are used in aquaculture by adding them to the water. Problems of palatability and leaching occur when the chemotherapeutic agents are incorporated in feeds. To circumvent these problems &lt;i&gt;Artemia&lt;/i&gt; can be used as a carrier. In this study we assess the efficacy of cotrimoxazole in the oral treatment of turbot larvae challenged with a pathogenic strain of Vibrio anguillarum encapsulated in &lt;i&gt;Artemia&lt;/i&gt;.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scophthalmus maximus (Linneaus; 1758).
Ano: 1995
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2536

An oral challenge for turbot larvae with &lt;i&gt;Vibrio anguillarum&lt;/i&gt;
Provedor de dados: Open Marine Archive
Autores: Chair, M.; Dehasque, M.; Van Poucke, S.; Nelis, H.; Sorgeloos, P.; De Leenheer, A.P.
There is a need for a standard in vitro procedure to infect larval fish with bacteria. Such a procedure could be used as a tool to evaluate fish quality, verify fish sensitivity to a certain bacterial strain or evaluate the pathogenicity of a bacterium towards a given fish species. The availability of a challenge test is a prerequisite in studies dealing with the effect of different medicinal treatments on diseased fish. An oral challenge test would offer the possibility to investigate the routes of infection via the digestive tract, i.e. after feeding contaminated feeds. The present study aims to verify this hypothesis and proposes a standard challenge procedure for turbot (&lt;i&gt;Scophthalmus maximus&lt;/i&gt;) larvae with a pathogenic &lt;i&gt;Vibrio...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial diseases; Digestive system; Disease control; Fish culture; Fish diseases; Fish larvae; Marine fish; Pathogenic bacteria; Vibrio anguillarum.
Ano: 1994
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2552

Bioencapsulation of the antibacterial drugs in nauplii and adult brine shrimp &lt;i&gt;Artemia franciscana&lt;/i&gt;
Provedor de dados: Open Marine Archive
Autores: Dixon, B.A.; Van Poucke, S.; Kawahigashi, D.; Chair, M.; Dehasque, M.; Nelis, H.J.; Sorgeloos, P.; De Leenheer, A.P.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemia franciscana.
Ano: 1995
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2562