Consulta: creatorFacets:"Charrier, Gregory"
Registros recuperados: 16
Data/hora: 01/06/2024 01:35:00

RAD sequencing sheds new light on the genetic structure and local adaptation of European scallops and resolves their demographic histories
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Vendrami, David L. J.; De Noia, Michele; Telesca, Luca; Handal, William; Charrier, Gregory; Boudry, Pierre; Eberhart-phillips, Luke; Hoffman, Joseph I.
Recent developments in genomics are advancing our understanding of the processes shaping population structure in wild organisms. In particular, reduced representation sequencing has facilitated the generation of dense genetic marker datasets that provide greater power for resolving population structure, investigating the role of selection and reconstructing demographic histories. We therefore used RAD sequencing to study the great scallop Pecten maximus and its sister species P. jacobeus along a latitudinal cline in Europe. Analysis of 219 samples genotyped at 82,439 single nucleotide polymorphisms clearly resolved an Atlantic and a Norwegian group within P. maximus as well as P. jacobeus, in support of previous studies. Fine-scale structure was also...
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00497/60901/64240.pdf

The Story of a Hitchhiker: Population Genetic Patterns in the Invasive Barnacle Balanus (Amphibalanus) improvisus Darwin 1854
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Wrange, Anna-lisa; Charrier, Gregory; Thonig, Anne; Rosenblad, Magnus Alm; Blomberg, Anders; Havenhand, Jonathan N.; Jonsson, Per R.; Andre, Carl.
Understanding the ecological and evolutionary forces that determine the genetic structure and spread of invasive species is a key component of invasion biology. The bay barnacle, Balanus improvisus (= Amphibalanus improvisus), is one of the most successful aquatic invaders worldwide, and is characterised by broad environmental tolerance. Although the species can spread through natural larval dispersal, human-mediated transport through (primarily) shipping has almost certainly contributed to the current global distribution of this species. Despite its worldwide distribution, little is known about the phylogeography of this species. Here, we characterize the population genetic structure and model dispersal dynamics of the barnacle B. improvisus, and describe...
Tipo: Text
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00616/72857/72859.pdf

AMP-deaminase in elasmobranch fish: A comparative histochemical and enzymatic study
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Thebault, Marie-t; Izem, Lahoucine; Leroy, Jean Paul; Gobin, Eric; Charrier, Gregory; Raffin, Jean-paul.
AMP-deaminase activity was measured in white muscle from a wide range of fish, including one cyclostome, 13 chondrosteans, and one teleost to elucidate the pattern of the AMP-deaminase activity in white muscle of fish. Compared to a mammalian (rat) muscle extract, low enzyme activities are found in the cyclostome and two elasmobranchs from two families (Scyliorhinidae, Hexanchidae). In contrast, higher AMP-deaminase activities, similar to mammals, are expressed in Squalidae, all families of skates, Chimaeridae and in the teleostean fish. We then compared AMP-deaminase activities in red and white muscles from two representative elasmobranch fish, the dogfish (Scyliorhinus canicula) and the thornback ray (Raja clavata). The fibre type composition and...
Tipo: Text
Palavras-chave: White fibres; Red fibres; Muscle; Histology; Fish; Evolution; Elasmobranch; AMP deaminase.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-635.pdf

Origin and route of establishment of the invasive Pacific oyster Crassostrea gigas in Scandinavia
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Faust, Ellika; Andre, Carl; Meurling, Sara; Kochmann, Judith; Christiansen, Henrik; Jensen, Lasse Fast; Charrier, Gregory; Laugen, Ane T.; Strand, Asa.
Identifying the routes and rates of introductions is fundamental for the understanding of marine invasions. Recurring introductions over the last 50 yr have led to the establishment of feral Pacific oyster Crassostrea gigas populations throughout Europe. In the northern countries, Sweden and Norway, the species first occurred in large numbers in 2006. Here, we investigated the relative importance of introduction via re-laying of cultured oysters imported for consumption from France, Ireland or the Netherlands, and dispersal of oyster larvae by ocean currents from wild oyster populations in Denmark. Using microsatellite DNA markers, we estimated genetic differentiation among Pacific oysters collected at 4 Swedish locations, 3 Norwegian locations and 9...
Tipo: Text
Palavras-chave: Population genetics; Microsatellites; Range expansion; Non-native species; Aquaculture; Connectivity; Scandinavia; Skagerrak.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00395/50627/72574.pdf

Variation in Size and Growth of the Great Scallop Pecten maximus along a Latitudinal Gradient
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Chauvaud, Laurent; Patry, Yann; Jolivet, Aurelie; Cam, Emmanuelle; Le Goff, Clement; Strand, Oivind; Charrier, Gregory; Thebault, Julien; Lazure, Pascal; Gotthard, Karl; Clavier, Jacques.
Understanding the relationship between growth and temperature will aid in the evaluation of thermal stress and threats to ectotherms in the context of anticipated climate changes. Most Pecten maximus scallops living at high latitudes in the northern hemisphere have a larger maximum body size than individuals further south, a common pattern among many ectotherms. We investigated differences in daily shell growth among scallop populations along the Northeast Atlantic coast from Spain to Norway. This study design allowed us to address precisely whether the asymptotic size observed along a latitudinal gradient, mainly defined by a temperature gradient, results from differences in annual or daily growth rates, or a difference in the length of the growing...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19861/17506.pdf

Genetic structure of a commercially exploited bivalve, the great scallop Pecten maximus, along the European coasts
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Morvezen, Romain; Charrier, Gregory; Boudry, Pierre; Chauvaud, Laurent; Breton, Florian; Strand, Oivind; Laroche, Jean.
The great scallop Pecten maximus is harvested in several European countries and fisheries targeting this species are severely regulated by fishing quotas. In addition, hatchery-based population enhancement has been developed in some countries to provide alternative or complementary production. The genetic structure of wild populations of P. maximus and the potential impact of aquaculture on the genetic diversity of this species remains poorly documented. In this study, we explored the genetic structure of P. maximus using 12 microsatellite markers, considering 14 populations sampled from Galicia (Spain) to the North of Norway, and one population of Pecten jacobaeus (L., 1758) from the Lion Gulf (Mediterranean Sea). Results indicated a clear differentiation...
Tipo: Text
Palavras-chave: Great scallop; Pecten maximus; Microsatellites; Population genetics; Aquaculture.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00311/42251/41692.pdf

Effects of bioactive extracellular compounds and paralytic shellfish toxins produced by Alexandrium minutum on growth and behaviour of juvenile great scallops Pecten maximus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Borcier, Elodie; Morvezen, Romain; Boudry, Pierre; Miner, Philippe; Charrier, Gregory; Laroche, Jean; Hegaret, Helene.
Dinoflagellates of the genus Alexandrium are a major cause of harmful algal blooms (HABs) that have increasingly disrupted coastal ecosystems for the last several decades. Microalgae from the genus Alexandrium are known to produce paralytic shellfish toxins (PST) but also bioactive extracellular compounds (BEC) that can display cytotoxic, allelopathic, ichtyotoxic or haemolytic effects upon marine organisms. The objective of this experimental study was to assess the effects of PST and BEC produced by A. minutum upon juvenile great scallops Pecten maximus. Scallops were exposed for one week to two different strains of A. minutum, the first producing both PST and BEC and the second producing only BEC. Escape response to starfish, daily shell growth,...
Tipo: Text
Palavras-chave: Harmful Algal Bloom (HAB); Alexandrium minutum; Pecten maximus; Paralytic Shellfish Toxins (PST); Extracellular compounds; Behaviour; Histology.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00368/47878/47893.pdf

The intra-annual variability of soft-bottom macrobenthos abundance patterns in the North Channel of the Seine estuary
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Desroy, Nicolas; Janson, Anne-laure; Denis, Lionel; Charrier, Gregory; Lesourd, Sandric; Dauvin, Jean-claude.
Temporal and spatial variability of the Abra alba–Pectinaria koreni and Macoma balthica communities was examined in the northern part of the Seine estuary (North Channel) over different space and time scales in order to assess the role that the hydrologic regime and/or anthropogenic influences play in defining benthic communities over time. Sediment in the North Channel displayed strong spatial and temporal variability, sustained by intense sediment transport episodes. Total macrobenthic abundances ranged widely on the course of the year and there was no evidence of a seasonal signal for the density fluctuations, whatever the spatial scale considered. The bio-sedimentary dynamics can be divided into two periods: the first corresponds to the high flow rate...
Tipo: Text
Palavras-chave: Port 2000; Anthropogenic impacts; Abra alba community; Sediment; Bentho pelagic coupling; Temporal variability.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2613.pdf

Population Genetic Structure is Unrelated to Shell Shape, Thickness and Organic Content in European Populations of the Soft-Shell Clam Mya Arenaria
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: De Noia, Michele; Telesca, Luca; Vendrami, David L. J.; Gokalp, Hatice K.; Charrier, Gregory; Harper, Elizabeth M.; Hoffman, Joseph I.
The soft-shell clam Mya arenaria is one of the most ancient invaders of European coasts and is present in many coastal ecosystems, yet little is known about its genetic structure in Europe. We collected 266 samples spanning a latitudinal cline from the Mediterranean to the North Sea and genotyped them at 12 microsatellite loci. In parallel, geometric morphometric analysis of shell outlines was used to test for associations between shell shape, latitude and genotype, and for a selection of shells we measured the thickness and organic content of the granular prismatic (PR), the crossed-lamellar (CL) and the complex crossed-lamellar (CCL) layers. Strong population structure was detected, with Bayesian cluster analysis identifying four groups located in the...
Tipo: Text
Palavras-chave: Mya arenaria; Soft-shell clam; Microsatellite; Population genetic structure; Phenotypic plasticity; Shell morphometrics.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00614/72600/71593.pdf

Insights from genetic and demographic connectivity for the management of rays and skates
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Marandel, Florianne; Lorance, Pascal; Andrello, Marco; Charrier, Gregory; Le Cam, Sabrina; Lehuta, Sigrid; Trenkel, Verena.
Studying demographic and genetic connectivity can help assessing marine meta-population structure. Rays and skates have no larval phase hence population connectivity can only result from active  movement of individuals. Using thornback ray (Raja clavata) in European waters as a case study, demographic  and genetic connectivity were studied for 11 putative populations for unequal population abundances and two  hypotheses of dispersal rates. Genetic simulation results highlighted three large meta-populations: in the  Mediterranean, around the Azores and on the Northeast Atlantic shelf. Demographic results highlighted a finer  population structure indicating that several pairs of putative populations might be demographically linked.  Results were highly...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00404/51535/52125.pdf

Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Borcier, Elodie; Artigaud, Sebastien; Gaillard, Jean-charles; Armengaud, Jean; Charrier, Gregory; Couteau, Jérôme; Receveur, Justine; Ouddane, Baghdad; Diop, Mamadou; Amara, Rachid; Laroche, Jean; Pichereau, Vianney.
Estuaries are important areas highly vulnerable to anthropogenic pollutions. Therefore, the assessment of estuarine water quality is a major ecological issue. In this study, we sampled juveniles of the European flounder in the “pristine” Canche estuary, and caged them in Canche and in two polluted sites of the Seine estuary, Rouen and Fosse Nord. After one month, the metal and organic pollutants in these sites were assessed, and we evaluated several phenotypic indicators (condition index, RNA/DNA ratios and genotoxicity), and extracted the proteins in fish livers for analysis using a shotgun proteomics approach. The results showed strong modifications in the fish caged in both sites of the Seine estuary, as compared to those caged in Canche. In particular,...
Tipo: Text
Palavras-chave: Estuary water quality; Ecotoxicology; Caging; Suess; Fish physiology; Shotgun proteomics.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00509/62067/66247.pdf

Estimating effective population size using RADseq: Effects of SNP selection and sample size
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Marandel, Florianne; Charrier, Gregory; Lamy, Jean-baptiste; Le Cam, Sabrina; Lorance, Pascal; Trenkel, Verena.
Effective population size (Ne) is a key parameter of population genetics. However, Ne remains challenging to estimate for natural populations as several factors are likely to bias estimates. These factors include sampling design, sequencing method, and data filtering. One issue inherent to the restriction site‐associated DNA sequencing (RADseq) protocol is missing data and SNP selection criteria (e.g., minimum minor allele frequency, number of SNPs). To evaluate the potential impact of SNP selection criteria on Ne estimates (Linkage Disequilibrium method) we used RADseq data for a nonmodel species, the thornback ray. In this data set, the inbreeding coefficient FIS was positively correlated with the amount of missing data, implying data were missing...
Tipo: Text
Palavras-chave: Effective population size; Linkage disequilibrium; NeEstimator; RADseq; Skates and rays.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00607/71934/70628.pdf

Impacts of ocean acidification and warming stress on juvenile growth and metabolism in two populations of Pecten maximus
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Harney, Ewan; Rastrick, Samuel; Artigaud, Sébastien; Pisapia, Julia; Miner, Philippe; Pichereau, Vianney; Strand, Oivind; Boudry, Pierre; Charrier, Gregory.
Elevated atmospheric CO2 is a major driver of global change in the ocean, causing sea surface temperatures to rise and acidification of marine environments. Together these stressors may have additive, synergistic or unforeseen consequences for many marine organisms, particularly during early life stages. The economically important King Scallop (Pecten maximus) is found along a large latitudinal gradient in the Eastern Atlantic, but it is unclear how this species will respond to changing environmental conditions. Studies of genetic structure suggest that Atlantic populations (from Spain to the UK) are genetically distinct from Norwegian populations, a result that is borne out by phenotypic differences. Yet whether genetic differences arise as a result of...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00404/51520/52114.pdf

Reconciling differences in natural tags to infer demographic and genetic connectivity in marine fish populations
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Reis-santos, Patrick; Tanner, Susanne E.; Aboim, Maria Ana; Vasconcelos, Rita P.; Laroche, Jean; Charrier, Gregory; Perez, Montse; Presa, Pablo; Gillanders, Bronwyn M.; Cabral, Henrique N.
Processes regulating population connectivity are complex, ranging from extrinsic environmental factors to intrinsic individual based features, and are a major force shaping the persistence of fish species and population responses to harvesting and environmental change. Here we developed an integrated assessment of demographic and genetic connectivity of European flounder Platichthys flesus in the northeast Atlantic (from the Norwegian to the Portuguese coast) and Baltic Sea. Specifically, we used a Bayesian infinite mixture model to infer the most likely number of natal sources of individuals based on otolith near core chemical composition. Simultaneously, we characterised genetic connectivity via microsatellite DNA markers, and evaluated how the combined...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00451/56210/72130.pdf

An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Borcier, Elodie; Charrier, Gregory; Couteau, Jérôme; Maillet, Géraldine; Le Grand, Fabienne; Bideau, Antoine; Waeles, Mathieu; Le Floch, Stephane; Amara, Rachid; Pichereau, Vianney; Laroche, Jean.
The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (Platichthys flesus) collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs. Douarnenez fish. The Seine fish displaying a reduced capacity to manage the oxidative stress and a higher energetic metabolism. An increase in the content of muscle membrane phospholipids (sphingomyelin, phosphatidylserine, free sterols) was detected in the Seine vs. Douarnenez fish. The data integration allowed to hypothesize relationships between...
Tipo: Text
Palavras-chave: Platichthys flesus; Estuaries; Biomarkers; Bioenergetics; Polar lipids; Population genetics.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00658/76981/78238.pdf

Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf