Consulta: creatorFacets:"Chereguini, Olvido"
Registros recuperados: 2
Data/hora: 04/06/2024 01:14:00

Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm
Provedor de dados: 5
Autores: Chereguini, Olvido; Cal, Rosa Maria; Dreanno, Catherine; Ogier De Baulny, Bénédicte; Suquet, Marc; Maisse, Gérard.
Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 degrees C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.
Tipo: Text
Palavras-chave: Turbot; Sperm; Storage; Cryopreservation.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00176/28724/27173.pdf

Effect of temperature, volume of ova batches, and addition of a diluent, an antibiotic, oxygen and a protein inhibitor on short-term storage capacities of turbot, Psetta maxima, ova
Provedor de dados: 5
Autores: Suquet, Marc; Chereguini, Olvido; Omnes, Marie-helene; Rasines, Inmaculada; Normant, Yvon; Souto, Isabel; Quemener, Loic.
The effect of different parameters on shea-term storage capacity of turbot ova was assessed over a 45-h period after ova collection for fertilization rates and over a 9-h period after ova collection for hatching rates. Increasing the volume of ova sampling from 0.5 to 2.5 mt, as well as adding an antibiotic-antimicotic solution or oxygen did not significantly change the storage capacity of ova. Regarding the hatching rates, a higher storage ability was recorded at 8 and 13 degrees C, compared to 3 degrees C. The mean composition of the ovarian fluid was determined (n = 57 spawns). Use of a diluent mimicking the ovarian fluid significantly decreased the storage ability as assessed by the fertilization rates but did not modify the hatching rates. Diluting...
Tipo: Text
Palavras-chave: Psetta maxima; Turbot; Calcium; Ovarian fluid; Short term storage; Ova; Fish; Psetta maxima; Turbot; Calcium; Liquide ovarien; Conservation à court terme; Ovules; Poisson.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/publication-857.pdf