Consulta: creatorFacets:"Chollet, Bruno"
Registros recuperados: 97
Data/hora: 04/06/2024 05:47:15

Molecular responses of Ostrea edulis haemocytes to an in vitro infection with Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms.  Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf

One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France
Provedor de dados: 5
Autores: Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence.
Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in...
Tipo: Text
Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf

Bilan 1998 du réseau REPAMO - Réseau National de Surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Chollet, Bruno; Cochennec, Nathalie; Renault, Tristan.
Le réseau REPAMO répond à plusieurs objectifs, définis dans différentes Directives Européennes: prévenir et détecter l'introduction et la propagation d'un agent pathogène émergent, réémergent ou exotique pour les productions de coquillages d'intérêt commercial en France, surveiller l'évolution des maladies à déclaration obligatoire, surveiller les échanges avec nos partenaires commerciaux. Le fonctionnement repose sur la collaboration entre les professionnels, la DPMCM, les laboratoires côtiers d'IFREMER, et trois cellules d'analyses. Les prélèvements et les outils diagnostiques choisis dépendent des différents objectifs. Le suivi est fait sur des populations sauvages et cultivées du littoral français. Le nombre d'analyses progresse régulièrement grâce à...
Tipo: Text
Palavras-chave: Réseau surveillance; Pathologie; Mortalités; Mollusques marins.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/00098/20904/18523.pdf

Culture d'huîtres creuses en eaux profondes dans le Pertuis d'Antioche. Comparaisons avec les cultures sur estran du bassin de Marennes Oléron et de Fouras et les filières d'élevage des Saumonards. Période avril 2001 - février 2002
Provedor de dados: 5
Autores: Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Guilpain, Patrice; Soletchnik, Patrick; Faury, Nicole; Seugnet, Jean-luc; Arnaud, Christophe; Chollet, Bruno; Chabirand, Jean-michel; Fleury, Pierre-gildas; Langlade, Aime; Goulletquer, Philippe; Mille, Dominique.
Le Bassin ostréicole de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1986), par un net recul des rendements de production qui engendrent une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est faite depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en  élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation défavorable pour la majorité des zones charentaises en terme de croissance-production est annuellement confirmée, depuis 1993 au moyen des réseaux de croissance...
Tipo: Text
Palavras-chave: Ostréiculture; Eaux profondes; Crassostrea gigas; Technique élevage; Croissance; Mortalité; Biométrie; Biochimie; Marennes Oléron.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00072/18369/15940.pdf

Culture d'huîtres creuses en eaux profondes dans le Pertuis d'Antioche - Comparaisons avec le site témoin de Fouras - Rapport préliminaire pour la période juillet 2000 - février 2001
Provedor de dados: 5
Autores: Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Guilpain, Patrice; Soletchnik, Patrick; Faury, Nicole; Seugnet, Jean-luc; Arnaud, Christophe; Chollet, Bruno; Chabirand, Jean-michel; Fleury, Pierre-gildas; Bouget, Jean-francois; Goulletquer, Philippe.
Il est important de rappeler ici les informations concernant le Projet "Culture d'huîtres creuses en eaux profondes dans le Pertuis d'Antioche" (Goulletquer, 2000). Le Bassin de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1985), par un net recul des rendements de production qui engendrent une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est démontrée depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation...
Tipo: Text
Palavras-chave: Ostréiculture; Eaux profondes; Crassostrea gigas; Technique élevage; Croissance; Mortalité; Pertuis Antioche.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00072/18370/15942.pdf

Le Réseau REPAMO : un outil de surveillance de la santé des mollusques marins élevés et sauvages
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Arzul, Isabelle; Robert, Maeva; Omnes, Emmanuelle; Chollet, Bruno; Renault, Tristan.
The Ifremer Repamo network (REseau de PAthologie des MOllusques) is the national surveillance network in charge of monitoring marine mollusc health on French coastlines. It is commissioned to enforce the official regulations and to provide a public service, under the supervision of the competent authority (DGAL Veterinary Directorate) in the Ministry.of Agriculture and Fisheries. Its goals are to prevent the introduction and the dissemination of infectious agents, in particular pathogens involved in reportable diseases, and to monitor the changes in the pathogens already present on the French coasts.
Tipo: Text
Palavras-chave: Network; Surveillance; Mollusks; Pathogens; Regulations.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6946.pdf

Bilan 2004 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Francois, Cyrille; Goubet, Anne; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Costantini, Louis; Masson, Jean-claude; Martin, Anne-genevieve.
Created in 1986, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Its activities are in keeping with two European Directives, 91 /67/EEC and 95/70/EC and are a patt of the institutional tasks ofIfremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to survey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Banc de Granville and zone X). In 2004, no sample couId be analysed to these two areas because of the difficulty to obtain flat oysters (sparse oyster beds,...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00086/19702/17343.pdf

A study of autophagy in hemocytes of the Pacific oyster,   Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf

Culture d'huîtres creuses en eaux profondes dans le Pertuis d'Antioche. Comparaison avec les cultures sur estran du bassin de Marennes Oléron et de Fouras et les filières d'élevage des Saumonards. Période avril 2002 - décembre 2002
Provedor de dados: 5
Autores: Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Guilpain, Patrice; Geairon, Philippe; Arnaud, Christophe; Chollet, Bruno; Faury, Nicole; Soletchnik, Patrick; Seugnet, Jean-luc; Mille, Dominique.
Le Bassin ostréicole de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1986), par un net recul des rendements de production qui engendre une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est démontrée depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation défavorable pour la majorité des zones charentaises en terme de « croissance - production» est annuellement confirmée, depuis 1993 au moyen des réseaux de suivi de...
Tipo: Text
Palavras-chave: Ostréiculture; Elevage en eaux profondes; Suivis expérimentaux; Biométrie; Mortalité; Bassin de Marennes Oléron; Fouras; Saumonards.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00134/24525/22559.pdf

Bilan 2015 du dispositif national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Lupo, Coralie; Osta Amigo, Axel; Fleury, Elodie; Robert, Stephane; Garcia, Celine; Arzul, Isabelle; Baillon, Laury; Bechemin, Christian; Canier, Lydie; Chollet, Bruno; Dechamps, Lucie; Dubreuil, Christine; Faury, Nicole; Francois, Cyrille; Godfrin, Yoann; Lapegue, Sylvie; Morga, Benjamin; Travers, Marie-agnes; Tourbiez, Delphine; Masson, Jean-claude; Verin, Francoise; Cordier, Remy; Gangnery, Aline; Louis, Wilfried; Mary, Charlotte; Normand, Julien; Penot, Julia; Cheve, Julien; Dagault, Francoise; Le Jolivet, Aurore; Le Gal, Dominique; Lebrun, Luc; Bellec, Gwenael; Bouget, Jean-francois; Cochennec-laureau, Nathalie; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Pepin, Jean-francois; Seugnet, Jean-luc; D'Amico, Florence; Maurer, Daniele; Le Gall, Patrik; Mortreux, Serge; Baldi, Yoann; Orsoni, Valerie; Bouchoucha, Marc; Le Roy, Valerian; Pouvreau, Stephane; Queau, Isabelle; Lamoureux, Alice.
Depuis 1992, la surveillance de la santé des mollusques marins du littoral français est assurée par le réseau de Pathologie des Mollusques (Repamo). Ses activités s’inscrivent dans le cadre de la Directive Européenne 2006/88/CE. Depuis son évaluation par la plateforme nationale d’épidémiosurveillance en santé animale en 2012, l’objectif de surveillance est la détection précoce des infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage. L’année 2015 est la première année de transition pour laquelle un début d’évolution des modalités de surveillance de la santé des mollusques marins animées par l’Ifremer a été amorcé. Un dispositif hybride de surveillance a été mis en place, s’appuyant sur...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Mortalité; Maladie.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00324/43486/42945.pdf

Réseau de Pathologie des Mollusques - Secteurs Nord-Loire - Année 1999. Bilan des travaux de la cellule zoosanitaire de La Trinité-sur-Mer
Provedor de dados: 5
Autores: Martin, Anne-genevieve; Tige, Gilbert; Hirata, Toshio; Le Coguic, Yvette; Chollet, Bruno; Robert, Maeva; Thebault, Anne.
Within the context of the mollusc pathology monitoring network, this report assesses the follow-up and work done by the zoosanitary unit of Trinité-sur-Mer on the whole of the French farming and bed areas of the North Loire for the year 1999.
Tipo: Text
Palavras-chave: Herpes virus; Pathology; Mortality; Britanny (France); Normandy; Clam; Abalone; Cockle; Mussel; Pacific cupped oyster; European flat oyster; Herpès virus; Pathologie; Mortalité; Bretagne; Normandie; Naissain; Palourde; Ormeau; Coque; Moule; Huître creuse; Huître plate.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/rapport-1921.pdf

Can Bonamia ostreae infect larvae of flat oysters Ostrea edulis ?
Provedor de dados: 5
Autores: Arzul, Isabelle; Robert, Maeva; Garcia, Celine; Chollet, Bruno; Langlade, Aime.
Tipo: Text
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00448/55999/57503.pdf

La Bonamiose : études des mécanismes cellulaires de défense de l'huître plate, Ostrea edulis, vis à vis du parasite Bonamia ostreae
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Chollet, Bruno.
Bonamiosis—a disease caused by a protozoan parasite, Bonamia ostreae—was detected for the first time in Brittany in 1979 in conjunction with a massive mortality event. Since then, the disease has spread to all French oyster farming areas, and then throughout Europe.
Tipo: Text
Palavras-chave: Pathologie; Ostrea edulis; Bonamia ostreae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3241.pdf

Comparative analysis of Vibrio splendidus-related strains isolated during Crassostrea gigas mortality events
Provedor de dados: 5
Autores: Le Roux, Frédérique; Gay, Melanie; Lambert, Christian; Waechter, Magali; Poubalanne, Saravanne; Chollet, Bruno; Nicolas, Jean-louis; Berthe, Franck.
French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar H strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast...
Tipo: Text
Palavras-chave: Vibrio splendidus; Virulence; Epidemiology; PCR RFLP; Oyster mortality.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-565.pdf

Le réseau Repamo : un outil de surveillance de la santé des mollusques marins élevés et sauvages (acte)
Provedor de dados: 5
Autores: Francois, Cyrille; Miossec, Laurence; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Robert, Maeva; Ferrand, Sylvie; Chollet, Bruno; Renault, Tristan.
Created in 1992, the Repamo network (REseau de PAthologie des MOllusques – Mollusc Pathology Network) is in charge of the surveillance and monitoring of the marine molluscs health status along the French coast. It has a regulatory mission (European legislation 91/67 and 95/70) as well as a public service activity under the supervision of the Ministry of Agriculture and Fisheries through the DPMA. It aims at preventing the introduction and propagation of pathogenic agents, in particular the noticeable agents, and monitoring the evolution of those already present on the national territory. These activities are part of the institutional missions of the Ifremer. The missions of the network are the following: 1) surveillance of the evolution of noticeable...
Tipo: Text
Palavras-chave: Mortalités; Maladies; Pathologie; Mollusques; Réseau surveillance; REPAMO.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3715.pdf

Avis sur la détection de parasites du genre Marteilia proche de l’espèce Marteilia cochillia chez les coques
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Serpin, Delphine; Noyer, Mathilde; Canier, Lydie; Bechemin, Christian.
Tipo: Text
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00602/71383/81205.pdf

Study of interactions between flat oyster, Ostrea edulis, and the parasite Bonamia ostreae using flow cytometry and suppression subtractrive hybridization (SSH)
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Gagnaire, Beatrice; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. Control of bonamiosis requires a good knowledge of the parasite life cycle including host-parasite interactions. In this study, we investigated interactions between parasites and haemocytes by flow cytometry and Suppression Subtractive Hybridization (SSH). In both approaches, analyses were performed after 2 hours of contact between haemocytes and parasites purified from highly infected flat oysters. Flow cytometry analyses consisted in testing haemocyte activities including esterase activities, reactive oxygen species (ROS) production and phagocytosis after contact with live parasites and parasites inactivated by...
Tipo: Text
Palavras-chave: SSH; Suppression subtractive hybridization; Cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3367.pdf

Suivi des gisements naturels de mollusques dans le cadre du réseau Repamo
Provedor de dados: 5
Autores: Garcia, Celine; Francois, Cyrille; Arzul, Isabelle; Miossec, Laurence; Joly, Jean-pierre; Robert, Maeva; Chollet, Bruno; Ferrand, Sylvie.
A monitoring of the natural and high density populations of commercial molluscs is performed on an annual basis within the scope of the Repamo mollusc pathology monitoring network The aim of this surveillance is (1) to monitor the health status of the molluscs along the French coast and to produce a reference picture, and (2) to study the evolution of the noticeable pathogenic agents existing on the national territory. Different sampling strategies have been introduced for the past 15 years; each strategy is based on an active monitoring including the systematic study of natural mollusc deposits. Indeed, the natural and high density mollusc populations are interrelated and pathogenic agents can be and have been exchanged between these populations with...
Tipo: Text
Palavras-chave: Haliotis tuberculata; Ostrea edulis; Population sauvage; Elevage; Mollusques; REPAMO; Réseau surveillance.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-3720.pdf

Summer mortality outbreaks of French Pacific oysters Crassostrea gigas in 2008 and 2009
Provedor de dados: 5
Autores: Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie.
Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August....
Tipo: Text
Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf

Bonamia ostreae-induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf

Rapport régional du REPAMO 1999: résultats de la cellule de veille zoosanitaire de La Tremblade dans les régions du sud de la Loire
Provedor de dados: 5
Autores: Thebault, Anne; Chollet, Bruno; Robert, Maeva.
La cellule de veille zoosanitaire de La Tremblade s'occupe de trois zones situées au sud de la Loire: la Baie de Bourgneuf, la région de Marennes-Oléron-Charentes, et le bassin d'Arcachon. Ces régions produisent principalement des huîtres creuses. Le naissain d'huîtres creuses produit à Arcachon et en Charente représente près de 80% de la production d'huître creuse, qui sera élevée dans toutes les régions ostréicoles françaises. Un effort particulier avec des prélèvements systématiques a donc été mis en place sur Crassostrea gigas dans ces trois trois zones, notamment sur les larves et le naissain d'Arcachon et de Charente. Compte tenu des transferts fréquents, des animaux sauvages sont prélevés pour caractériser les sites, tandis que la production en...
Tipo: Text
Palavras-chave: Réseau surveillance; REPAMO; Analyses zoosanitaires; Huître creuse; Crassostrea gigas; Mortalités.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00134/24488/22513.pdf

Haemocytes from Crassostrea gigas and OsHV-1: A promising in vitro system to study host/virus interactions
Provedor de dados: 5
Autores: Morga, Benjamin; Faury, Nicole; Guesdon, Stephane; Chollet, Bruno; Renault, Tristan.
Since 2008, mass mortality outbreaks associated with the detection of particular variants of OsHV-1 have been reported in Crassostrea gigas spat and juveniles in several countries. Recent studies have reported information on viral replication during experimental infection. Viral DNA and RNA were also detected in the haemolymph and haemocytes suggesting that the virus could circulate through the circulatory system. However, it is unknown if the virus is free in the haemolymph, passively associated at the surface of haemocytes, or able to infect and replicate inside these cells inducing (or not) virion production. In the present study, we collected haemocytes from the haemolymphatic sinus of the adductor muscle of healthy C. gigas spat and exposed them in...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00399/50997/51780.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 96 RPC R70 - Programme Pathologie - Rapport Scientifique année 1996
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Lipart, Cecile; Thebault, Anne.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00043/15452/12827.pdf

Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text
Palavras-chave: Hémocytes; Ostrea edulis; Bonamia ostreae; Parasite; Huîtres.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3368.pdf

Molecular characterization of parasites of the genus Perkinsus present in clams from French producing areas
Provedor de dados: 5
Autores: Arzul, Isabelle; Michel, Justine; Chollet, Bruno; Robert, Maeva; Miossec, Laurence; Garcia, Celine; Francois, Cyrille.
Production of clams is the third most important bivalve production in France and was estimated at 3400 metric tons in 2001 among which 60% came from aquaculture and 40% from fisheries. Surveillance of clam diseases mainly relies on histology and thioglycolate culture, two techniques allowing detection of parasites of the genus Perkinsus but not specific enough to determine the parasite species. Based on a previous study aiming at estimating distribution and prevalence of perkinsosis in France, we selected 34 infected clams from 4 different producing areas in order to characterize the parasite species. Molecular characterization was done by PCR-RFLP targeting the ITS region as described by Abollo et al. (2006). 16 PCR products were cloned and 10 to 20...
Tipo: Text
Palavras-chave: French producing areas; Clams; Perkinsus olseni; Parasite.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/acte-6269.pdf

Haemocytes collected from experimentally infected Pacific oysters, Crassostrea gigas: Detection of ostreid herpesvirus 1 DNA, RNA, and proteins in relation with inhibition of apoptosis
Provedor de dados: 5
Autores: Martenot, Claire; Gervais, Ophelie; Chollet, Bruno; Houssin, Maryline; Renault, Tristan.
Recent transcriptomic approaches focused on anti-viral immunity in molluscs lead to the assumption that the innate immune system, such as apoptosis, plays a crucial role against ostreid herpesvirus type 1 (OsHV-1), infecting Pacific cupped oyster, Crassostrea gigas. Apoptosis constitutes a major mechanism of anti-viral response by limiting viral spread and eliminating infected cells. In this way, an OsHV-1 challenge was performed and oysters were monitored at three times post injection to investigate viral infection and host response: 2h (early after viral injection in the adductor muscle), 24h (intermediate time), and 48h (just before first oyster mortality record). Virus infection, associated with high cumulative mortality rates (74% and 100%), was...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00386/49715/50244.pdf

Acclimatation de nouvelles espèces d'huîtres creuse du genre Crassostrea: hybridation et conservatoire de souches - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Synthèse finale
Provedor de dados: 5
Autores: Boudry, Pierre; Heurtebise, Serge; Huvet, Arnaud; Chollet, Bruno; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Actions menées de 1994 à 1998 au sein du conservatoire de souches : 1. Résultats des importations, acclimatation et hybridation. a) Rappel des conditions d'introduction de nouvelles souches. L'importation de toute espèce est conditionnée par les résultats d'une enquête préliminaire dont l'objet est de rechercher les exigences écologiques de l'espèce et la situation épidémiologique dans son milieu d'origine. Le travail du LGP et de ses partenaires a été partagé en différentes actions: 1. Recherche de contacts dans divers pays étrangers, en vue de l'importation d'huîtres présentant des caractéristiques intéressantes. 2. Importation : L'importation se fait selon les normes du CIEM (Conseil International pour l'Exploration de la Mer) : contrôles pathologiques...
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Marqueurs moléculaire; Marqueurs populations; Crassostrea.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15520/12907.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Rapport année 1996
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée).
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf

Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Concomitant sporadic high mortalities were reported in France in May 1994 among batches of hatchery-reared larval Pacific oysters Crassostrea gigas and European flat oysters Ostrea edulis in 2 hatcheries, and in June and July 1994 among batches of cultured spat of both species in a shellfish nursery. Histological observation showed the presence of cellular abnormalities in moribund animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in infected larvae and spat of both oyster species. This is the first description of a herpes-like virus infection in larval O.. edulis. Viruses observed in diseased larvae and spat of both species are similar with respect to ultrastructure and morphogenesis, They were detected...
Tipo: Text
Palavras-chave: Apoptosis; Virus replication; Concomitant infection; Oyster mortality; Crassostrea gigas; Pacific oyster; Ostrea edulis; European flat oyster; Herpes like virus.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-820.pdf

Bonamia-like parasite found in the Suminoe oyster Crassostea rivularis reared in France
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,...
Tipo: Text
Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf

Infection with the protozoan parasite Bonamia ostreae modifies in vitro haemocyte activities of flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan.
Bonamia ostreae is an intracellular protozoan parasite, infecting haemocytes of the European flat oyster Ostrea edulis. Oyster defence mechanisms mainly rely on haemocytes. In the present study in vitro interactions between parasites and flat oyster haemocytes were investigated using flow cytometry and light microscopy. Haemocyte parameters including: non specific esterase activity, reactive oxygen species (ROS) production and phagocytosis were monitored using flow cytometry after 2 hour cell incubation with live and dead B. ostreae. Two ratios of parasites per haemocyte were tested (5:1 and 10:1), haemocytes alone were used as controls and the experiment was carried out three times. Flow cytometry revealed a decrease of non specific esterase activities...
Tipo: Text
Palavras-chave: Reactive oxygen species (ROS); Esterase; Flow cytometry; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6271.pdf

Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text
Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf

Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water
Provedor de dados: 5
Autores: Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text
Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf

Juvenile oyster disease (JOD) in American oysters, Crassostrea virginica, reared in France. Detection of abnormal calcium deposits in gill and mantle tissues
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared during 1992 and 1993, to test their growth performances. These assays were performed to investigate the possible replacement of Crassostrea gigas with a non indigenous species, Crassostrea virginica. During the summer of 1993, sporadic mass mortalities (80-90%) occurred among young, Crassastrea virginica, oysters reared in the IFREMER nursery in La Tremblade (Charente Maritime).
Tipo: Text
Palavras-chave: Histology; Crassostrea virginica; Mortality; Oysters.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2975.pdf

REPAMO: a surveillance tool for mollusc health
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva.
At the present time losses due to diseases are one of main factors limiting the aquaculture development. Unceasing increase of exchanges between different countries favours the risk of introduction and propagation of new pathogen agents. At the beginning of the 90’s the European Union laid the surveillance of mollusc health inside each Member State in order to protect the safety of exchanges. Indeed few measures are available to protect molluscs from diseases. Only the prophylactic approach is possible; this approach is based on a zoosanitary surveillance of mollusc populations and/or a development of resistant populations against some diseases. Created in 1986, the French network for surveillance and monitoring of mollusc health (REPAMO) ensures the...
Tipo: Text
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00442/55334/56826.pdf

Diagnostic des pathologies rencontrées chez les mollusques bivalves marins
Provedor de dados: 5
Autores: Chollet, Bruno.
L'huître creuse, Crassostrea gigas, est aujourd'hui une des espèces de mollusques bivalves marins la plus élevée dans le monde. Cette espèce a été importée du Japon, dans les années 1960-1970, et a remplacé l'huître portugaise, Crassostrea angulata, espéce décimée à cette époque par deux iridoviroses. Suite à la disparition de l'huître portugaise du littoral français et à l'apparition de protozooses (Marteiliose et Bonamiose) chez l'huître plate, Ostrea edulis, une situation de quasi monoélevage de l'huître japonaise s'est installée en France. De ce fait, les risques sur les plans sanitaire et économique sont loin d'être négligeables pour le milieu ostréicole français. En particulier, l'apparition de maladies chez cette espèce pourrait aboutir à une...
Tipo: Text
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00429/54085/55402.pdf

Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin.
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members...
Tipo: Text
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf

PERLE - Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis - Rapport final des sous-programmes 2 et 3
Provedor de dados: 5
Autores: Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie.
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente...
Tipo: Text
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf

Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia
Provedor de dados: 5
Autores: Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010...
Tipo: Text
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 95 RPC R 62 - Programme Pathologie - Rapport Scientifique année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe; Le Deuff, Rose-marie; Thuillier, Benoit.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00043/15453/12828.pdf

Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia
Provedor de dados: 5
Autores: Lopez Flores, L; Suarez Santiago, V; Longet, D; Saulnier, Denis; Chollet, Bruno; Arzul, Isabelle.
Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and...
Tipo: Text
Palavras-chave: Molecular phylogeny; Actin gene; Ostrea edulis parasite; Bonamia ostreae.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3562.pdf

Bilan 2007 du réseau REPAMO - Réseau national de surveillace de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Ferrand, Sylvie; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier; D'Amico, Florence; Rumebe, Myriam; Pichot, Yves; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Ravel, Christophe.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de deux Directives Européennes, les Directives 91 /67/CEE et 95/70/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae et la Marteiliose à Marteilia refringens chez l'huître...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Mollusques; Coquillages; Santé.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00086/19708/17349.pdf

Contribution of in Vivo Experimental Challenges to Understanding Flat Oyster Ostrea edulis Resistance to Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle.
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf

Bilan 2009 du réseau REPAMO - Réseau national de surveillace de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Cobret, Laetitia; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Breerette, Stephane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Banamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00086/19711/17352.pdf

Etude du virus de type herpes observé chez les huîtres
Provedor de dados: 5
Autores: Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Delsert, Claude; Cochennec, Nathalie; Renault, Tristan.
Since 1991, sporadic, but high mortality in larvae of the Pacific oyster (Crassostrea gigas) has been observed during the summer in various French hatcheries. This mortality was concomitant with the detection of a virus by transmission electron microscopy. This virus, by virtue of its morphological characteristics and developmental cycle, seems to belong to the Herpesviridae family. The pathogenicity of this viral agent has been demonstrated in the laboratory by experimentally induced mortality on healthy larvae. Moreover, concomitant mortality has been reported in larvae of Crassostrea gigas and Ostrea edulis reared in the same tanks, with detection of a herpes-type virus.
Tipo: Text
Palavras-chave: Virus; Herpeviridae; Pathologie; Crassostrea gigas.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/1997/acte-3248.pdf

Descriptions of Mikrocytos veneroïdes n. sp. and Mikrocytos donaxi n. sp. (Ascetosporea: Mikrocytida: Mikrocytiidae), detected during important mortality events of the wedge clam Donax trunculus Linnaeus (Veneroida: Donacidae), in France between 2008 and 2011
Provedor de dados: 5
Autores: Garcia, Celine; Haond, Christophe; Chollet, Bruno; Nerac, Mirella; Omnes, Emmanuelle; Joly, Jean-pierre; Dubreuil, Christine; Serpin, Delphine; Langlade, Aime; Le Gal, Dominique; Terre-terrillon, Aouregan; Courtois, Olivier; Guichard, Benjamin; Arzul, Isabelle.
Background: Microcell parasites are small intracellular protozoans mostly detected in molluscs and can be associated with mortalities. In 2010 and 2011, strong increases in mortality events were reported in different wild beds of the wedge clam Donax trunculus Linnaeus, along the Atlantic coast of France and the presence of potential pathogens, including microcells, was investigated. Methods: Clams collected in different beds showing mortality were examined by histology. Based on histological observations, confirmatory analyses were carried out, including transmission electron microscopy (TEM) and molecular characterization. Results: Histological analyses revealed the presence of small protozoans similar to microcell parasites in different tissues of Donax...
Tipo: Text
Palavras-chave: Mikrocytos veneroides n. sp.; Mikrocytos donaxi n. sp.; Donax trunculus; Mortality; Microcell parasite.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00428/53989/55149.pdf

Note de synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Avril-Mai 1996
Provedor de dados: 5
Autores: Renault, Tristan; Lipart, Cecile; Le Deuff, Rose-marie; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade dans le cadre de l'étude du virus de type herpès et de la mise au point de méthodes de diagnostic fait suite à la précédente note du 16 avril 1996. Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont donnés et commentés en sept points. 1- Analyses Pays de Loire 2 - Bilan des analyses réalisées dans le cadre du protocole IFREMER BOUIN/IFREMER ARGENTON/SODABO 3 - Bilan global des analyses Pays de Loire et Bouin-Argenton-SODABO 4 - Analyses effectuées en PCR pour la recherche de virus de type herpès sur des lots prélevés en 1996, dans le...
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15499/12867.pdf

Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa
Provedor de dados: 5
Autores: Prado-alvarez, Maria; Couraleau, Yann; Chollet, Bruno; Tourbiez, Delphine; Arzul, Isabelle.
Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where...
Tipo: Text
Palavras-chave: Bonamia exitiosa; Bonamia ostreae; Actin; Haplosporidia; Whole-Genome Amplification.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00277/38871/37410.pdf

Bonamia-like parasite found in the Suminoe oyster Crassostrea rivularis reared in France
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production. Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-Like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea....
Tipo: Text
Palavras-chave: Crassostrea rivularis; Suminoe oyster; Bonamiosis; Bonamia-like parasite.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/00446/55795/62652.pdf

Development of a TaqMan PCR assay for the detection of Bonamia species
Provedor de dados: 5
Autores: Corbeil, Serge; Arzul, Isabelle; Diggles, Ben; Heasman, Mike; Chollet, Bruno; Berthe, Franck; Crane, Mark St. J.
The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New...
Tipo: Text
Palavras-chave: Oyster; Real time TaqMan PCR assay; Bonamia spp.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1980.pdf

Bonamia-ostreae induced mortalities in one-year old European flat oysters Ostrea edulis: experimental infection by cohabitation challenge
Provedor de dados: 5
Autores: Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie.
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to...
Tipo: Text
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf

Bilan 2013 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Tourbiez, Delphine; Chollet, Bruno; Faury, Nicole; Haffner, Philippe; Dubreuil, Christine; Serpin, Delphine; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Cheve, Julien; Penot, Julia; Le Gal, Dominique; Lebrun, Luc; Le Gac-abernot, Chantal; Langlade, Aime; Bedier, Edouard; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrick; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Repamo a pour objectif de détecter précocement les infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage.  L’étude des hausses de mortalités a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la collecte d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités d’huîtres creuses (Crassostrea gigas) (44 interventions) ont été recensées dans la majorité des bassins de production de mai à...
Tipo: Text
Palavras-chave: Réseau Surveillance; Pathologie; Mollusques; Coquillages; Santé.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00197/30798/29167.pdf

New evidence for the involvement of Paracartia grani (Copepoda, Calanoida) in the life cycle of Marteilia refringens (Paramyxea)
Provedor de dados: 5
Autores: Boyer, S.; Chollet, Bruno; Bonnet, D.; Arzul, Isabelle.
The dynamics of the protozoan parasite Marteilia refringens was studied in Thau lagoon, an important French shellfish site, for 1 year in three potential hosts: the Mediterranean mussel Mytilus galloprovincialis (Mytiliidae) the grooved carpet shell Ruditapes decussatus (Veneriidae) and the copepod Paracartia grani (Acartiidae). Parasite DNA was detected by PCR in R. decussatus. In situ hybridisation showed necrotic cells of M. refringens in the digestive epithelia of some R. decussatus suggesting the non-involvement of this species in the parasite life cycle. In contrast, the detection of M. refringens in mussels using PCR appeared bimodal with two peaks in spring and autumn. Histological observations of PCR-positive mussels revealed the presence of...
Tipo: Text
Palavras-chave: Marteiliosis; Marteilia refringens; Parasite life cycle; Mytilus galloprovincialis; Ruditapes decussatus; Paracartia grani; PCR; In situ hybridisation.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26795/25532.pdf

Contribution to the understanding of the cycle of the protozoan parasite Marteilia refringens
Provedor de dados: 5
Autores: Arzul, Isabelle; Chollet, Bruno; Boyer, S.; Bonnet, D.; Gaillard, Juliette; Baldi, Yoann; Robert, Maeva; Joly, Jean-pierre; Garcia, Celine; Bouchoucha, Marc.
The paramyxean parasite Marteilia refringens infects several bivalve species including European flat oysters Ostrea edulis and Mediterranean mussels Mytilus galloprovincialis. Sequence polymorphism allowed definition of three parasite types ‘M’, ‘O’ and ‘C’ preferably detected in oysters, mussels and cockles respectively.Transmission of the infection from infected bivalves to copepods Paracartia grani could be experimentally achieved but assays fromcopepods to bivalves failed. In order to contribute to the elucidation of the M. refringens life cycle, the dynamics of the infection was investigated in O. edulis, M. galloprovincialis and zooplankton over one year in Diana lagoon, Corsica (France). Flat oysters appeared non-infected while mussels were infected...
Tipo: Text
Palavras-chave: Bivalves; Marteilia refringens; Copepods; Paracartia latisetosa; Zooplankton; Parasite life cycle.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00165/27635/25768.pdf

Heat shock protein 90 of Bonamia ostreae: characterization and possible correlation with infection of the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Prado Alvarez, Maria; Chollet, Bruno; Couraleau, Yann; Morga, Benjamin; Arzul, Isabelle.
In this study we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis haemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae infected haemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in haemocyte invasion. Our results inform the molecular basis that governs B. ostreae-O. edulis interactions.
Tipo: Text
Palavras-chave: Bivalve haemocytes; Gene; Haplosporidia; Pathogen; Protist; Radicicol.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24799/22878.pdf

Ostrea conchaphila : a natural host of Bonamia ostreae ?
Provedor de dados: 5
Autores: Arzul, Isabelle; Chollet, Bruno; Garcia, Celine; Robert, Maeva; Joly, Jean-pierre; Miossec, Laurence; Berthe, Franck.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00448/55998/57502.pdf

Bilan 2012 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Pepin, Jean-francois; Hatt, Philippe-jacques; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Huchet, Eve; Dubreuil, Christine; Chollet, Bruno; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Annezo, Jean-pierre; Terre-terrillon, Aouregan; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Hittier, Benoist; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude.
Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à réglementés et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font partie des missions institutionnelles de l’IFREMER.   L’étude des hausses de mortalités (protocole II) a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la réalisation d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités...
Tipo: Text
Palavras-chave: Réseau; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00143/25470/23625.pdf

Rapport sur la situation zoosanitaire du cheptel Crassostrea gigas : cas du bassin de Marennes-Oléron
Provedor de dados: 5
Autores: Grizel, Henri; Renault, Tristan; Cochennec, Nathalie; Tige, Gilbert; Audic, Guylaine; Pichot, Yves; Chollet, Bruno; Goulletquer, Philippe; Soletchnik, Patrick; Geairon, Philippe; Prou, Jean.
Le cheptel d'huîtres creuses, Crassostrea gigas, du Bassin de Marennes-Oléron a subi ces dernières années des morta lités (mai 88, mai 91, été 1992 et hiver 92-93). Les examens histopathologiques pratiqués durant celles-ci n'ont pas permis de déceler d'agents pathogènes. Les causes avaient été attribuées essentiellement, pour les premiers cas, à un manque de nourriture corrélé avec des conditions de sécheresse (donc un manque d'apport d'eau douce pour le bassin) et à des surcharges trop importantes des biomasses de fiItreurs, huîtres y compris. L'explication du dernier cas de mortalité survenue au cours de l'hiver serait d'un autre ordre: l'abaissement brutal des salinités, notamment dans les claires où étaient épurées soit des huîtres natives fragilisées,...
Tipo: Text
Palavras-chave: Etude zoosanitaire; Mortalité; Epidémiologie; Pathologie; Marennes Oléron.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00050/16152/13635.pdf

Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov.
Provedor de dados: 5
Autores: Garcia, Celine; Mesnil, Aurelie; Tourbiez, Delphine; Moussa Pouly, Mirna; Dubreuil, Christine; Gonçalves De Sa, Amélie; Chollet, Bruno; Godfrin, Yoann; Dégremont, Lionel; Serpin, Delphine; Travers, Marie-agnes.
Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover,...
Tipo: Text
Palavras-chave: Bacterial pathogen; Bivalvia; Cockle; Mortality; Shellfish; Vibrio aestuarianus.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00677/78893/81195.pdf

Cosmopolitan Distribution of Endozoicomonas-Like Organisms and Other Intracellular Microcolonies of Bacteria Causing Infection in Marine Mollusks
Provedor de dados: 5
Autores: Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W.
Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed....
Tipo: Text
Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf

Programme Pathologie - Rapport scientifique Année 1994 - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Convention 94 RPC R 62
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno; Maffart, Patricia; Haffner, Philippe; Berthe, Franck; Thuillier, Benoit.
Le but de ce programme pathologie est de recherher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassastrea gigas, afin d'identifier d'éventuels agents causant des mortalité, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socioéconomique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la pathologie....
Tipo: Text
Palavras-chave: Pathologie; Agent pathogène; Infection; Virus; Herpèsvirus; Purification; Huître creuse; Crassostrea gigas.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/00044/15502/12874.pdf

Ostreid herpesvirus 1 detection and relationship with Crassostrea gigas spat mortality in France between 1998 and 2006
Provedor de dados: 5
Autores: Garcia, Celine; Thebault, Anne; Degremont, Lionel; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno; Francois, Cyrille; Joly, Jean-pierre; Ferrand, Sylvie; Kerdudou, Nolwenn; Renault, Tristan.
Since its molecular characterisation, Ostreid herpesvirus 1 (OsHV-1) has been regularly detected in Crassostrea gigas in France. Although its pathogenicity was demonstrated on larval stages, its involvement during mortality outbreaks at the juvenile stage was highly suspected but not evidenced. To investigate mortality outbreaks, the French National Network for Surveillance and Monitoring of Mollusc Health (REPAMO) carried out two surveys in juvenile C. gigas. The first survey lasted from 1998 to 2006 and was an epidemiological inquiry occurring when oyster farmers reported mortality outbreaks. The second survey, a longitudinal one, was set up in 1998 to complete the network observations on OsHV-1. Data analysis showed a specific pattern of mortality...
Tipo: Text
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15110/12572.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 98 RPC R 7 - Programme Pathologie - Rapport Final année 1998
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Cochennec, Nathalie; Haffner, Philippe.
Depuis 1991, des mortalités massives sporadiques sont rapportées, en France, de manière régulière chez des larves et des juvéniles de différentes espèces de mollusques bivalves marins. Certains de ces épisodes de mortalité ont pu être associés à la détection de virus. Ces derniers par leur morphologie, leur cycle de réplication et leur localisation ont été apparentés à la famille des Herpesviridae. Cependant, les outils de diagnostic disponibles jusqu'alors (histologie et microscopie électronique à transmission) n'étaient pas adaptés à l'étude de ces virus du fait de leur manque de spécificité et de la lourdeur de leur mise en oeuvre. Par ailleurs, se posait la question de l'implication des virus détectés dans les phénomènes de mortalité observés. En...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00043/15455/12830.pdf

Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae?
Provedor de dados: 5
Autores: Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle.
Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to...
Tipo: Text
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf

Bilan 2008 du réseau REPAMO - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Tourbiez, Delphine; Omnes, Emmanuelle; Cobret, Laetitia; Faury, Nicole; Haffner, Philippe; Saulnier, Denis; Pepin, Jean-francois; Renault, Tristan; Antajan, Elvire; Rauflet, Fabienne; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00086/19709/17351.pdf

REPAMO : a French network for the surveillance of molluscs health
Provedor de dados: 5
Autores: Guichard, Benjamin; Garcia, Celine; Joly, Jean-pierre; Lupo, Coralie; Chollet, Bruno; Robert, Maeva; Omnes, Emmanuelle; Arzul, Isabelle.
The REPAMO is a French Network for the surveillance of Mollusc diseases that notably meets the obligations of the European Directive 2006/088/EC. It is entirely funded by the French State. The coordination of this network entrusted to the Institut Français de Recherche pour l`Exploitation de la Mer since 1986 aims at monitoring the notifiable endemic diseases, detecting the emergence of exotic or new pathogens and ensuring a minimum level of surveillance of the general health condition of molluscs. Beside IFREMER, different partners are involved in the surveillance of mollusc health at the national level: the Departmental direction for territories and sea who are the local representatives of the competent authority and the producers who have the obligation...
Tipo: Text
Palavras-chave: Surveillance; Mollusc; Pathology; Network.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00036/14768/12077.pdf

Bilan 2010 du réseau Repamo - Réseau national de surveillance de la santé des mollusques marins
Provedor de dados: 5
Autores: Guichard, Benjamin; Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Brerette, Stéphane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam; Cantin, Christian.
Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l’IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l’huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Surveillance.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00059/17050/14599.pdf

Marteiliosis in American oysters Crassostrea virginica reared in France
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno.
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared at 3 different locations. A survey was taken of these young C, virginica oysters during 1993. Histological and electron microscopical examination revealed a parasite interpreted as a Marteilia-like protozoan in 1 oyster. The structure and the initial stages of the developmental cycle of this parasite are described for the first time in American oysters. However, no mature spores were detected. Thus, exact identification of the protozoan is difficult. This study indicates that American oysters could be infected with Marteilia spp, during cohabitation with Marteilia-infected native bivalves in France.
Tipo: Text
Palavras-chave: American oyster; Crassostrea virginica; Marteiliosis; Marteilia-like parasite.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00258/36926/36177.pdf

Etude des intéractions entre le parasite Bonamia ostreae et son hôte, huître plate Ostrea edulis in vitro (Diaporama)
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan.
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in...
Tipo: Text
Palavras-chave: Intéraction; Hémocytes; Ostrea edulis; Bonamia ostreae; Parasitologie.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3366.pdf

Herpes-like virus infecting Japanese oyster (Crassostrea gigas) spat
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno.
During the summer of 1993, abnormal sporadic mass mortalities (80-90%) and morbidities occurred among four batches of young Japanese oyster (Crassostrea gigas) spat from the French Atlantic coast. These mortalities were only observed in July. Samples of affected animaIs were examined by electron microscopy. This paper reports the presence and appearance of herpes viruses in these samples.
Tipo: Text
Palavras-chave: Herpes like virus; Mortalities; Crassostrea gigas; Oysters.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-2888.pdf

Compte rendu de la réunion des 14 et 15 Avril 1993 - La Tremblade - Réseau Génétique Mollusques (REGEMO)
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Coatanea, Denis; Martin, Anne-genevieve; Barret, Jean; Claude, Serge; Heurtebise, Serge; Devauchelle, Nicole; Joly, Jean-pierre; Goulletquer, Philippe; Baud, Jean-pierre; Nourry, Max; Bougrier, Serge; Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno; Le Deuff, Rose-marie; Peignon, Jean-marie.
La discussion générale organisée à la fin de la deuxième journée a fait ressortir les points suivants: 1- Les participants se sont déclarés satisfaits de la tenue de la réunion, tant dans son déroulement, que dans les échanges d'informations auxquels elle a donné lieu. La quantité et la qualité des résultats présentés a permis de mettre en évidence la puissance d'analyse que représente le travail en réseau. 2-L'importance de la valorisation des résultats a été soulignée à plusieurs reprises. La diffusion de l'information devrait se faire dans un premier temps par la voie de rapport internes à la DRV, puis par la voie d'articles publiés dans des revues scientifiques. Sur le programme "C. gigas triploïdes", une première diffusion des résultats obtenus en...
Tipo: Text
Palavras-chave: Génétique; Sélection génétique; Huître plate; Ostrea edulis; Génétique population; Croissance.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00049/15981/13423.pdf

Flow cytometry to measure impact of temperature and salinity on the survival of Bonamia ostreae, parasite infecting flat oyster Ostrea edulis, in seawater
Provedor de dados: 5
Autores: Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979&amp;#305; in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely...
Tipo: Text
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf

Bilan 2006 du réseau Repamo. Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Francois, Cyrille; Garcia, Celine; Labrut, Sophie; Miossec, Laurence; Arzul, Isabelle; Joly, Jean-pierre; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie.
Created in 1992, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Its activities are in keeping with two European Directives, 91/67/EEC and 95/70/EC and are a part of the institutional tasks of Ifremer. The aims of the network are to prevent the introduction and spread of exotic  pathogens and to survey the evolution ofnotifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (zone X and Granville bed in zone IX). In 2006, only samples of the Granville bed could be analysed due to the difficulty to obtain flat oysters (sparse oyster...
Tipo: Text
Palavras-chave: Health status; Shellfish; Molluscs; Pathology; Surveillance; Network; État de santé; Coquillages; Mollusques; Pathologie; Surveillance; Réseau.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/rapport-3812.pdf

Bilan 2005 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier; D'Amico, Florence; Rumebe, Myriam; Pichot, Yves; Le Gall, Patrik.
Created in 1992, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Ils activities are in keeping with two European Directives, 91167/EEC and 95170/EC and are a part of the institutional tasks of Ifremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to sm'vey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Granville bed and zone X). In 2005, only samples of the Granville bed could be analysed due to the difflculty to obtain flat oysters (sparse oyster beds, beds...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00086/19704/17346.pdf

Synthèse des résultats obtenus concernant l'étude du virus de type herpès et la mise au point de méthodes de diagnostic de l'infection - Juin-Septembre 1996
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Cette note de synthèse des travaux réalisés au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type . herpès et de la mise au point des méthodes de diagnostic fait suite à la précédente note (fin mai 1996). Les résultats obtenus concernant les analyses protocolaires et hors protocoles, ainsi que les résultats expérimentaux déjà disponibles sont données et commentés en quatre points. Cette étude est soutenue par la Région Poitou-Charente et la Région Pays de Loire.
Tipo: Text
Palavras-chave: Pathologie; Virus; Herpès virus; Infection; Diagnostic; Huître creuse; Crassostrea gigas.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00044/15503/12875.pdf

Compte-rendu des résultats d'analyses effectuées en histologie - Année 1995
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno.
Dans le cadre du Réseau zoosanitaires CEE (huître plate) et suivi d'autres espèces, 890 individus provenant des zones 5, 6 et 7 ont été examinés sur l'ensemble de l'année 1995 par différentes techniques : 472 analyses sous coupes histologiques, 328 analyses sur appositions d'organes, 90 analyses sur milieu au thioglycolate.
Tipo: Text
Palavras-chave: Pathologie; Analyse; Histologie; Technique; Bivalves; Ostrea edulis; Huître plate; Mytilus edulis; Ruditapes philippinarum; Ruditapes decussatus; Cerastoderma edule; Mortalité.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15504/12877.pdf

Repamo network : a surveillance tool for mollusc health
Provedor de dados: 5
Autores: Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Francois, Cyrille; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva.
At the present time losses due to diseases are one of main factors limiting the aquaculture development. Unceasing increase of exchanges between different countries favours the risk of introduction and propagation of new pathogen agents. At the beginning of the 90's the European Union laid the surveillance of mollusc health inside each Member State in order to protect the safety of exchanges. Indeed few measures are available to protect molluscs from diseases. Only the prophylactic approach is possible; this approach is based on a zoosanitary surveillance of mollusc populations and/or a development of resistant populations against some diseases. Created in 1986, the French network for surveillance and monitoring of mollusc health (REPAMO) ensures the...
Tipo: Text
Palavras-chave: Marteiliosis; Bonamiosis; Organisation; Mission; REPAMO; Surveillance; Pathogenic agents; Mollusc health.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3315.pdf

Molecular detection and quantification of the protozoan Bonamia ostreae in the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle.
Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of &lt;1% and a minimum detection limit of 50 gene copies per reaction. Using...
Tipo: Text
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf

Contrat de Plan Etat Région Poitou-Charentes 1994-1998. Convention 97 RPC R 58 - Programme Pathologie - Rapport Scientifique année 1997
Provedor de dados: 5
Autores: Renault, Tristan; Berthe, Franck; Thebault, Anne; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe; Cochennec, Nathalie.
Le but de ce programme pathologie est de rechercher et d'étudier les agents pathogènes connus et inconnus chez les mollusques bivalves marins, plus particulièrement chez l'huître creuse, Crassostrea gigas, afin d'identifier d'éventuels agents causant des mortalités, de proposer des mesures prophylactiques et de définir des conditions zootechniques permettant aux professionnels de limiter l'impact des maladies sur leur cheptel. La présence de maladies, chez les mollusques bivalves marins dont l'impact socio économique peut être catastrophique pour les productions conchylicoles, et l'accroissement des échanges de produits d'aquaculture entre les pays de la CEE et avec les pays tiers nécessitent le renforcement des recherches dans les domaines de la...
Tipo: Text
Palavras-chave: Pathologie; Pathogènes; Virus; Herpès virus; Crassostrea gigas; Diagnostic; Herpesviridae.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00043/15454/12829.pdf

Programme Herpèsvirus. Synthèse des travaux réalisés au Laboratoire IFREMER de La Tremblade - Mars 1996 - Février 1997
Provedor de dados: 5
Autores: Renault, Tristan; Le Deuff, Rose-marie; Lipart, Cecile; Chollet, Bruno; Haffner, Philippe.
Ce rapport représente la synthèse des travaux réalisés de mars 1996 à février 1997 au laboratoire de Génétique, Aquaculture et Pathologie de la station IFREMER de La Tremblade, dans le cadre de l'étude du virus de type herpès observé chez les huîtres et de la mise au point de méthodes de diagnostic. La majorité des travaux rapportés ici de façon complète, a déjà fait l'objet de comptes rendus intermédiaires.
Tipo: Text
Palavras-chave: Pathologie; Herpèsvirus; Herpesviridae; Huître creuse; Crassostrea gigas; Huître plate; Ostrea edulis; Virus; Détection; ¨Protocole; ADN; PCR.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00165/27663/25838.pdf

Etude des agents pathogènes et épidémiologie
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Robert, Maeva; Chollet, Bruno; Gerard, Andre.
Le programme 4 portant sur la pathologie des coquillages dans le cadre d'une gestion durable des productions ostréicoles, comporte 2 actions principales réparties en une étude d'épidémiologie analytique et descriptive des agents pathogènes (Action 1), et l'étude des relations hôtes/agents pathogènes (Action 2). Les résultats 2001 présentés ici sont relatifs à ces 2 actions, avec notamment des informations sur les infections à virus de type herpès, et de recherche de bactéries pathogènes chez Crassostrea gigas lors des épisodes de mortalités estivales. Par ailleurs, l'émergence d'un programme national, intitulé MOREST (pour Mortalités Estivales) sur l' huître creuse C. gigas va permettre de rappeler le contexte scientifique des relations hôtes/pathogènes.
Tipo: Text
Palavras-chave: Pathologie; Agents pathogènes; Epidémiologie; Huître creuse; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00199/31011/29414.pdf

Bilan 2000 du réseau REPAMO - Réseau National de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Thebault, A; Robert, M.; Chollet, Bruno; Dumais, Muriel; Joly, Jean-pierre; Garcia, C.; Comtet, Thierry; Le Coguic, Matthieu; Martin, Anne-genevieve; Pichot, Yves.
Tipo: Text
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00080/19111/16706.pdf

Bilan 2003 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins
Provedor de dados: 5
Autores: Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Kopp, Joel; Mouillard, Gilbert; Gerla, Daniel; Le Saux, Jean-claude; Raguenes, Pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Fillon, Alain.
The REPAMO network (network ofmollusc pathology), created in 1986, ensures the survey of shellfish health status along French coasts according? to European Directives 91/67/EEC and 95/70/EEC. The aims of the network are 1 - to prevent the introduction and spread of exotic pathogens, 2-to study the means to decrease the impact of pathogens already present and to survey their evolution. In 2003, the REPAMO network focused on the survey oflisted diseases (bonamiosis and marteiliosis) in two areas under agreement process (Banc de Granville and zone X) and on the study of abnormal morlalities. Number of reported abnormal morlalities has increased in 2003. This rise can be partly explained by the recent network restructuring, but also by the summer heatwave and...
Tipo: Text
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00086/19699/17340.pdf

Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridations et conservatoire de souches. Contrat de Plan Etat Région Poitou- Charentes 1994-1998 - Convention 95/RPC-R-57 "Génétique" - Rapport année 1995
Provedor de dados: 5
Autores: Boudry, Pierre; Naciri, Yamama; Heurtebise, Serge; Delsert, Claude; Ledu, Christophe; Phelipot, Pascal; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous les membres du Réseau Génétique Mollusques (REGEMO), a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou-Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses.
Tipo: Text
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea virgnica.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00044/15515/12902.pdf

Marteilia in American oysters Crassostrea virginica reared in France
Provedor de dados: 5
Autores: Renault, Tristan; Cochennec, Nathalie; Chollet, Bruno.
Progeny of Crassostrea virginica oysters, introduced into France in 1992, were reared at 3 different locations. A survey was taken of these young C. virginica oysters during 1993. Histological and electron microscopical examination revealed a parasite interpreted as a Martelia-like protozoan in 1 oyster. The structure and the initial stages of the developmental cycle of this parasite are described for the first time in American oysters. However, no mature spores were detected. Thus, exact identification of the protozoan is difficult. This study indicates that American oysters could be infected with Marteilia spp. during cohabitation with Marteilia-infected native bivalves in France.
Tipo: Text
Palavras-chave: Marteilia like parasite; Marteiliosis; Crassostrea virginica; American oyster.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/publication-2897.pdf

Viral gametocytic hypertrophy of Crassostrea gigas in France: from occasional records to disease emergence?
Provedor de dados: 5
Autores: Garcia, Celine; Robert, Maeva; Arzul, Isabelle; Chollet, Bruno; Joly, Jean-pierre; Miossec, Laurence; Comtet, Thierry; Berthe, Franck.
Viral gametocytic hypertrophy was reported for the first time in 2001 in Pacific oyster Crassostrea gigas in France. Since this date, the number of reported cases and the distribution area have increased every year; however, the cases are not associated with macroscopic signs or increased mortality rates. Both male and female gametes were hypertrophied and basophilic inclusions were observed in gamete nuclei, Transmission electron microscopy revealed the presence of viral particles in these intranuclear basophilic inclusions. These particles had characteristics similar to those of the Papillomaviridae and Polyomaviridae families: they were small, non-enveloped, icosahedral, and 44 to 56 nm in diameter, The viral particles were found in male, female and...
Tipo: Text
Palavras-chave: Polyomaviridae; Papillomaviridae; Gonad; Viral garnetocytic hypertrophy; Pacific oyster; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-3288.pdf

Comparison of Heamograms from resistant and susceptible European flat oysters, Ostrea edulis, exposed to the parasite, Bonamia ostreae
Provedor de dados: 5
Autores: Cochennec, Nathalie; Renault, Tristan; Chollet, Bruno; Maffart, Patricia.
To study the cellular defence mechanisms involved in the resistance of the flat oysters against Bonamia ostreae, a comparaison of haemograms was performed between susceptible and resistant animals
Tipo: Text
Palavras-chave: Pathogen; Bonamia ostreae; Ostrea edulis.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/1995/acte-2906.pdf

Cellular and molecular responses of haemocytes from Ostrea edulis during in vitro infection by the parasite Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Chollet, Bruno; Arzul, Isabelle.
Bonamia ostreae is a protozoan, affiliated to the order Haplosporidia and to the phylum Cercozoa. This parasite is intracellular and infects haemocytes, cells notably involved in oyster defence mechanisms. Bonamiosis due to the parasite B. ostreae is a disease affecting the flat oyster, Ostrea edulis. The strategies used by protozoan parasites to circumvent host defence mechanisms remain largely unknown in marine bivalve molluscs. In the present work, in vitro experiments were carried out in order to study the interactions between haemocytes from O. edulis and purified parasite. B. ostreae. We monitored cellular and molecular responses of oyster haemocytes by light microscopy, flow cytometry and real-time PCR 1, 2, 4 and 8 h p.i. Light microscopy was used...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Haemocytes; Real-time PCR; Flow cytometry; Super oxide dismutase.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14874/18001.pdf

Infection of clams by Perkinsus olseni as an example of the new French Surveillance Programme
Provedor de dados: 5
Autores: Miossec, Laurence; Garcia, Celine; Arzul, Isabelle; Francois, Cyrille; Joly, Jean-pierre; Chollet, Bruno; Robert, Maeva; De Blas, Isabelle.
The French network for the surveillance and monitoring of mollusc health (REPAMO) was officially set up in 1992 to control the shellfish status along the French coasts according to the European directives 91/67/EEC and 95/70/EC. The objectives are 1) the surveillance of notifiable diseases present in France (Bonamiosis and Marteiliosis of flat oysters Ostrea edulis), 2) the surveillance of health status of cultured and wild populations of molluscs, 3) the study of abnormal mortality and 4) the control of exchanges from European and third countries. In this context, a new surveillance programme, called Active Targeted Survey, part of the REPAMO, was applied in 2004 in order to improve the monitoring of the health status of the French mollusc populations...
Tipo: Text
Palavras-chave: Surveillance; Bivalvia; Clams; Perkinsus olseni; Pathogen; Pathology.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-6376.pdf

Can the protozoan parasite Bonamia ostreae infect larvae of flat oysters Ostrea edulis?
Provedor de dados: 5
Autores: Arzul, Isabelle; Langlade, Aime; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie; Omnes, Emmanuelle; Lerond, Sophie; Couraleau, Yann; Joly, Jean-pierre; Francois, Cyrille; Garcia, Celine.
Bonamia ostreae is an intracellular protistan parasite affecting flat oysters Ostrea edulis. It can be detected in juveniles but mortalities mainly affect oysters which are more than 2 years old. The parasite is usually observed inside haemocytes and sometimes free, notably in gill epithelia suggesting a parasite release through this organ. However, the infective form and ways of entry and release remain undetermined. Flat oysters incubate their larvae in their pallial cavity for 8–10 days before releasing them into the water column. Flat oysters in Bay of Quiberon in South Brittany (France) are known to be infected with B. ostreae since 1979 and is the most important area in France for O. edulis spat collection. Flat oysters incubating larvae were sampled...
Tipo: Text
Palavras-chave: Bonamia ostreae; Flat oyster; Ostrea edulis; Transmission; Larvae; Parasite life cycle.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00028/13898/12566.pdf

Réseau de veille zoosanitaire des mollusques REPAMO - Rapport d'activité pour l'année 1997
Provedor de dados: 5
Autores: Berthe, Franck; Pichot, Yves; Chollet, Bruno; Thebault, Anne; Kuntz, Gregoire; Tige, Gilbert; Thebault, Anne; Fleury, Pierre-gildas; Hirata, Toshio; Le Coguic, Yvette; Renault, Tristan; Lipart, Cecile; Grizel, Henri; Joly, Jean-pierre; Mazurie, Joseph; Baud, Jean-pierre; Goulletquer, Philippe; Dreno, Jean-paul; Buestel, Dominique.
Le réseau de veille zoosanitaire des mollusques, REPAMO, a été créé en 1992 afin de protéger les espèces d'intérêt économique des maladies susceptibles de les affecter. En effet, de même que les autres productions animales, la conchyliculture est frappée par diverses maladies dont certaines se comportent comme des facteurs limitants de la qualité ou de la quantité des productions. L' histoire de l'ostréiculture française a connu de tristes expériences en la matière, notamment dans les années 70 pour l' huître creuse portugaise, Crassostrea angulata ou encore pour l'huître plate, Ostrea edulis. En fait, nous ne disposons que de très peu de possibilités de protéger les mollusques bivalves vis à vis des maladies infectieuses. D' une part, les traitements,...
Tipo: Text
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00098/20901/18521.pdf

Bilans depuis 1999 et évolution de la stratégie du REPAMO
Provedor de dados: 5
Autores: Thebault, Anne; Martin, Anne-genevieve; Pichot, Yves; Chollet, Bruno; Robert, Maeva; Le Coguic, Yvette; Tige, Gilbert; Hirata, Toshio; Mazurie, Joseph; Le Saux, Jean-claude; Masson, Nadine; Auby, Isabelle; Maurer, Daniele; Le Roux, Frédérique; Cochennec, Nathalie; Renault, Tristan; Berthe, Franck; Grizel, Henri.
The main objectives of the Mollusc Pathology Network (REPAMO) are to prevent introductions or outbreaks of infectious agents, prevent spread within a farm and especially from one farm to another, to study ways of decreasing the impact of infectious agent and to monitor the development of these pathogens in order to guarantee the health status of oysters exchanged with our commercial partners. On the whole, REPAMO's activities have not changed and include: - monitoring the development of diseases that have to be reported to authorities and carrying out analyses to classify zones: bonamiosis and marteiliosis; - baseline monitoring of farmed and wild populations of bivalve molluscs; - studying cases of abnormal mortality events; - controlling mollusc...
Tipo: Text
Palavras-chave: Mollusques; Réseau surveillance; Pathologie; REPAMO.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-3265.pdf

Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast
Provedor de dados: 5
Autores: Renault, Tristan; Stokes, Nancy; Chollet, Bruno; Cochennec, Nathalie; Berthe, Franck; Gerard, Andre; Burreson, Eugene.
Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A...
Tipo: Text
Palavras-chave: Haplosporidium nelsoni; Haplosporidiosis; Crassostrea gigas; Pacific oyster.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-821.pdf

Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae
Provedor de dados: 5
Autores: Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle.
The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses....
Tipo: Text
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf

Effects of temperature and salinity on the survival of Bonamia ostreae, a parasite infecting flat oysters Ostrea edulis
Provedor de dados: 5
Autores: Arzul, Isabelle; Gagnaire, Beatrice; Bond, Celine; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l–1. Parasites were collected after 12, 24 and 48 h of...
Tipo: Text
Palavras-chave: Salinity tolerance; Temperature tolerance; Cell viability; In vitro assays; Flow cytometry; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6443.pdf

Epidémiologie analytique et descriptive des agents pathogènes, étude de la diversité des virus de type herpès (action 1). Etude des relations hôtes/agents pathogènes, pathogénèse des infections à virus de type herpèsvirus (action 2) - Contrat Région Poitou-Charentes 2000-2006
Provedor de dados: 5
Autores: Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno.
Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés.
Tipo: Text
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf

Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy)
Provedor de dados: 5
Autores: Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G.
The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,...
Tipo: Text
Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf