Consulta: creatorFacets:"Coelho,Irene da Silva"
Registros recuperados: 5
Data/hora: 11/06/2024 02:30:02

Morphological and molecular characterization of Pisolithus in soil under eucalyptus plantations in Brazil
Provedor de dados: 90
Autores: Kasuya,Maria Catarina Megumi; Coelho,Irene da Silva; Campos,Daniela Tiago da Silva; Araújo,Elza Fernandes de; Tamai,Yutaka; Miyamoto,Toshizumi.
Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained by phylogenetic relationship based on internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of these isolates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ectomycorrhizal fungus; Phylogenetic analysis; Internal transcribed spacer (ITS); Nuclear ribosomal DNA; Taxonomy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000600013

Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.
Provedor de dados: 52
Autores: Coelho,Irene da Silva; Queiroz,Marisa Vieira de; Costa,Maurício Dutra; Kasuya,Maria Catarina Megumi; Araújo,Elza Fernandes de.
The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regeneration was achieved (8.5 %); 80.0 % of protoplasts were nucleated, and 20.0 % anucleated. For Ceratorhiza sp., the largest number of protoplasts (4.0 × 10(7) protoplasts/mL) was achieved in 0.6 M NaCl, when 15 mg/mL of Lysing Enzymes and 15mg/mL of Glucanex, with 2-day-old fungal mycelium were used. The highest frequency of regeneration was 6.7 % using 0.5 M sucrose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ceratorhiza; Epulorhiza; Molecular biology; Protoplast; Regeneration.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100019

Impairments of mecA gene detection in bovine Staphylococcus spp.
Provedor de dados: 58
Autores: Melo,Dayanne Araújo de; Coelho,Irene da Silva; Motta,Cássia Couto da; Rojas,Anna Carolina Coelho Marín; Dubenczuk,Felipe Carlos; Coelho,Shana de Mattos de Oliveira; Souza,Miliane Moreira Soares de.
Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the conserved regions from distinct host origins and also investigated the presence of homologue mecA LGA251 in bovine strains. A total of 43 Staphylococcus spp. were included in this study, comprising 38 bovine S. aureus, two human and three equine coagulase-negative staphylococci (CNS). Phenotypical methicillin-resistance detection was performed through oxacillin agar-screening and cefoxitin disk-diffusion test. None isolate tested positive for mecA LGA251 gene. For mecA gene PCR, new primers were designed based on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial resistance; Bovine mastitis; Staphylococccus; MecA gene.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300041

Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis
Provedor de dados: 58
Autores: Marques,Viviane Figueira; Motta,Cássia Couto da; Soares,Bianca da Silva; Melo,Dayanne Araújo de; Coelho,Shana de Mattos de Oliveira; Coelho,Irene da Silva; Barbosa,Helene Santos; Souza,Miliane Moreira Soares de.
Abstract Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Agr types; Antimicrobial resistance; Mastitis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118

The Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) identification versus biochemical tests: a study with enterobacteria from a dairy cattle environment
Provedor de dados: 58
Autores: Rodrigues,Naiara Miranda Bento; Bronzato,Greiciane França; Santiago,Gabrielli Stefaninni; Botelho,Larissa Alvarenga Batista; Moreira,Beatriz Meurer; Coelho,Irene da Silva; Souza,Miliane Moreira Soares de; Coelho,Shana de Mattos de Oliveira.
Abstract Mastitis adversely affects milk production and in general cows do not regain their full production levels post recovery, leading to considerable economic losses. Moreover the percentage decrease in milk production depends on the specific pathogen that caused the infection and enterobacteria are responsible for this greater reduction. Phenotypic tests are among the currently available methods used worldwide to identify enterobacteria; however they tend to misdiagnose the species despite the multiple tests carried out. On the other hand The Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) technique has been attracting attention for its precise identification of several microorganisms at species level. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine mastitis; Enterobacteria identification; Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100132