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Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
Provedor de dados: 124
Autores: Castro,Ana Hortência Fonsêca; Braga,Karina de Queiroz; Sousa,Fernanda Marinez de; Coimbra,Mairon César; Chagas,Rafael César Russo.
ABSTRACT This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 µM) and BAP (0; 4.44; 8.88; 17.75 µM ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Medicinal plant; In vitro culture; Secondary metabolites.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000100143